Klebsiella phage vB_Kpn_F48

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Marfavirus; Klebsiella virus F48

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6UFG6|A0A2I6UFG6_9CAUD Uncharacterized protein OS=Klebsiella phage vB_Kpn_F48 OX=2070028 GN=vBKpnF48_31 PE=4 SV=1
MM1 pKa = 6.64TTEE4 pKa = 3.83QKK6 pKa = 11.32AEE8 pKa = 3.97MFRR11 pKa = 11.84LIDD14 pKa = 3.96EE15 pKa = 4.59YY16 pKa = 11.69VGAHH20 pKa = 6.22YY21 pKa = 10.24AYY23 pKa = 9.01TWLADD28 pKa = 3.94GGWDD32 pKa = 4.81CDD34 pKa = 5.45DD35 pKa = 4.95EE36 pKa = 5.93GLDD39 pKa = 5.6HH40 pKa = 7.25ANAWNALKK48 pKa = 10.84DD49 pKa = 4.52FINGLL54 pKa = 3.72

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6UFL0|A0A2I6UFL0_9CAUD Anti-sigma factor OS=Klebsiella phage vB_Kpn_F48 OX=2070028 GN=vBKpnF48_153 PE=4 SV=1
MM1 pKa = 7.54TIDD4 pKa = 3.07TRR6 pKa = 11.84NYY8 pKa = 5.53MTRR11 pKa = 11.84GDD13 pKa = 3.88LTKK16 pKa = 10.87AGAVTVAHH24 pKa = 6.38VKK26 pKa = 10.34NGHH29 pKa = 4.72TVGFVMPEE37 pKa = 3.72VLAQPGFYY45 pKa = 10.62FMVKK49 pKa = 9.97GAGEE53 pKa = 4.1NRR55 pKa = 11.84TVGARR60 pKa = 11.84FFVGNQRR67 pKa = 11.84SAQGFNATLSHH78 pKa = 6.24IRR80 pKa = 11.84QGRR83 pKa = 11.84SQLARR88 pKa = 11.84TMASNNIVYY97 pKa = 9.64EE98 pKa = 4.04VLYY101 pKa = 10.69LPQSKK106 pKa = 9.18MKK108 pKa = 10.23PLTTGFKK115 pKa = 10.26KK116 pKa = 10.76GQLALAFTRR125 pKa = 11.84QHH127 pKa = 5.92SSEE130 pKa = 4.07MQTLSEE136 pKa = 4.67LNRR139 pKa = 11.84ILNDD143 pKa = 3.0NFKK146 pKa = 10.97FILQSYY152 pKa = 8.66

Molecular weight: 16.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

283

0

283

52666

37

1403

186.1

21.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.373 ± 0.19

1.088 ± 0.058

6.264 ± 0.11

7.244 ± 0.156

4.263 ± 0.123

6.298 ± 0.21

1.829 ± 0.083

6.929 ± 0.13

7.305 ± 0.169

7.494 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.076

5.142 ± 0.141

3.465 ± 0.093

3.498 ± 0.094

4.587 ± 0.096

6.255 ± 0.138

5.767 ± 0.201

6.841 ± 0.114

1.42 ± 0.057

4.113 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski