Vicia cryptic virus
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A3R740|A3R740_9VIRU Coat protein OS=Vicia cryptic virus OX=54289 GN=CP PE=4 SV=1
MM1 pKa = 8.35 DD2 pKa = 4.02 YY3 pKa = 10.79 LISAFNRR10 pKa = 11.84 ITHH13 pKa = 6.2 WFITPTNFEE22 pKa = 4.52 YY23 pKa = 10.24 IGNFSLPPGLLRR35 pKa = 11.84 VNDD38 pKa = 3.63 VAIANHH44 pKa = 6.43 KK45 pKa = 8.33 CTLEE49 pKa = 4.19 RR50 pKa = 11.84 SFHH53 pKa = 6.3 TYY55 pKa = 10.89 LFDD58 pKa = 4.72 HH59 pKa = 7.13 EE60 pKa = 4.96 IKK62 pKa = 10.5 RR63 pKa = 11.84 IMIDD67 pKa = 3.03 HH68 pKa = 6.73 RR69 pKa = 11.84 RR70 pKa = 11.84 SDD72 pKa = 3.31 ITEE75 pKa = 4.04 DD76 pKa = 4.04 SILEE80 pKa = 4.1 DD81 pKa = 4.32 FFAGDD86 pKa = 3.55 FPYY89 pKa = 10.81 FEE91 pKa = 4.8 VPFDD95 pKa = 3.47 EE96 pKa = 4.41 HH97 pKa = 8.55 VEE99 pKa = 4.07 YY100 pKa = 10.84 GLQCMADD107 pKa = 3.51 AFRR110 pKa = 11.84 PPRR113 pKa = 11.84 PCRR116 pKa = 11.84 PAHH119 pKa = 6.19 ILDD122 pKa = 3.78 VKK124 pKa = 10.61 HH125 pKa = 6.44 GYY127 pKa = 8.11 PYY129 pKa = 10.43 KK130 pKa = 10.06 WNVNAEE136 pKa = 4.36 PPFSTDD142 pKa = 3.38 EE143 pKa = 4.07 YY144 pKa = 11.14 FLTQRR149 pKa = 11.84 KK150 pKa = 6.66 TFGEE154 pKa = 5.1 FIRR157 pKa = 11.84 MHH159 pKa = 6.57 EE160 pKa = 4.21 YY161 pKa = 10.71 EE162 pKa = 5.95 HH163 pKa = 7.09 IDD165 pKa = 2.9 KK166 pKa = 11.07 DD167 pKa = 3.99 DD168 pKa = 3.55 FFRR171 pKa = 11.84 RR172 pKa = 11.84 HH173 pKa = 6.25 PNLEE177 pKa = 3.7 SHH179 pKa = 7.24 DD180 pKa = 4.02 FLRR183 pKa = 11.84 TIVPPKK189 pKa = 10.3 FGYY192 pKa = 10.37 LKK194 pKa = 9.81 STIFSWTRR202 pKa = 11.84 RR203 pKa = 11.84 WHH205 pKa = 6.57 HH206 pKa = 6.35 IIKK209 pKa = 10.58 LGFTDD214 pKa = 4.06 TTGLDD219 pKa = 3.64 NNGYY223 pKa = 10.02 LYY225 pKa = 11.06 NRR227 pKa = 11.84 FIFPMLLHH235 pKa = 6.66 TKK237 pKa = 8.25 TAIVKK242 pKa = 10.22 KK243 pKa = 10.36 DD244 pKa = 3.95 DD245 pKa = 4.07 PNKK248 pKa = 9.93 MRR250 pKa = 11.84 TIWGASKK257 pKa = 9.86 PWIIADD263 pKa = 3.44 TMFYY267 pKa = 10.22 WEE269 pKa = 3.73 YY270 pKa = 10.07 QAWVKK275 pKa = 10.49 HH276 pKa = 5.59 NPGSTPMLWGFEE288 pKa = 4.25 TFTGGWFRR296 pKa = 11.84 LNQLLFCGLIRR307 pKa = 11.84 RR308 pKa = 11.84 SFITLDD314 pKa = 2.77 WSRR317 pKa = 11.84 FDD319 pKa = 3.15 KK320 pKa = 10.78 RR321 pKa = 11.84 AYY323 pKa = 10.21 FPLLRR328 pKa = 11.84 KK329 pKa = 9.74 IMYY332 pKa = 7.15 TVKK335 pKa = 10.63 SFLTFEE341 pKa = 4.03 EE342 pKa = 6.21 GYY344 pKa = 10.49 VPTHH348 pKa = 6.91 AAPNHH353 pKa = 5.48 PQWNQDD359 pKa = 3.0 KK360 pKa = 9.59 TDD362 pKa = 3.37 KK363 pKa = 11.32 LEE365 pKa = 4.89 RR366 pKa = 11.84 LWLWTLEE373 pKa = 4.18 NLFEE377 pKa = 4.94 APIILPDD384 pKa = 2.89 GRR386 pKa = 11.84 MYY388 pKa = 10.65 RR389 pKa = 11.84 RR390 pKa = 11.84 HH391 pKa = 5.78 FAGIPSGLFITQLLDD406 pKa = 2.7 SWYY409 pKa = 9.85 NYY411 pKa = 9.3 TMLATILHH419 pKa = 6.57 ALGFNPSNCIIKK431 pKa = 10.11 VQGDD435 pKa = 3.54 DD436 pKa = 3.82 SIIRR440 pKa = 11.84 LNVLVPSEE448 pKa = 4.1 RR449 pKa = 11.84 HH450 pKa = 5.0 DD451 pKa = 3.66 HH452 pKa = 5.31 LMSRR456 pKa = 11.84 IVEE459 pKa = 3.9 LAEE462 pKa = 4.18 YY463 pKa = 9.65 YY464 pKa = 10.69 FNSIVNVEE472 pKa = 3.75 KK473 pKa = 11.17 SEE475 pKa = 3.71 IRR477 pKa = 11.84 NRR479 pKa = 11.84 LNGCEE484 pKa = 3.81 VLSYY488 pKa = 10.62 RR489 pKa = 11.84 NHH491 pKa = 6.44 NGLPFRR497 pKa = 11.84 DD498 pKa = 4.47 EE499 pKa = 3.98 IAMLAQFYY507 pKa = 7.91 HH508 pKa = 6.12 TKK510 pKa = 10.61 ARR512 pKa = 11.84 DD513 pKa = 3.58 PTPEE517 pKa = 3.33 ITMAQAIGFAYY528 pKa = 10.18 ASCATHH534 pKa = 6.83 TRR536 pKa = 11.84 VLWVLEE542 pKa = 4.51 DD543 pKa = 3.15 IYY545 pKa = 11.41 NYY547 pKa = 10.59 YY548 pKa = 9.92 RR549 pKa = 11.84 DD550 pKa = 3.28 QGYY553 pKa = 7.49 TPNRR557 pKa = 11.84 AGLTLTFGDD566 pKa = 4.55 SPDD569 pKa = 3.29 LTMPEE574 pKa = 4.14 MPLDD578 pKa = 3.84 HH579 pKa = 7.26 FPTKK583 pKa = 10.63 SEE585 pKa = 3.49 IVRR588 pKa = 11.84 YY589 pKa = 7.53 LTCTNYY595 pKa = 10.3 RR596 pKa = 11.84 NEE598 pKa = 4.12 AQNARR603 pKa = 11.84 TWPRR607 pKa = 11.84 TLFINAPAEE616 pKa = 3.97
Molecular weight: 72.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.304
IPC2_protein 6.249
IPC_protein 6.376
Toseland 6.737
ProMoST 6.707
Dawson 6.737
Bjellqvist 6.707
Wikipedia 6.751
Rodwell 6.722
Grimsley 6.912
Solomon 6.737
Lehninger 6.737
Nozaki 7.015
DTASelect 7.19
Thurlkill 7.22
EMBOSS 7.19
Sillero 7.117
Patrickios 4.024
IPC_peptide 6.751
IPC2_peptide 6.985
IPC2.peptide.svr19 6.904
Protein with the highest isoelectric point:
>tr|A3R740|A3R740_9VIRU Coat protein OS=Vicia cryptic virus OX=54289 GN=CP PE=4 SV=1
MM1 pKa = 7.36 EE2 pKa = 4.96 AHH4 pKa = 6.24 TPAADD9 pKa = 3.52 VNGPNIPSGAVSQPEE24 pKa = 4.09 IAPHH28 pKa = 5.56 NQAAPNVSGAAQAIIAPTPARR49 pKa = 11.84 KK50 pKa = 8.18 RR51 pKa = 11.84 TPHH54 pKa = 6.06 GPIPAATSSGNTSAPALLEE73 pKa = 3.9 LAAAYY78 pKa = 9.36 PMYY81 pKa = 10.13 TEE83 pKa = 4.07 QRR85 pKa = 11.84 RR86 pKa = 11.84 SFNFFIPDD94 pKa = 3.38 SQMMFHH100 pKa = 7.04 SLGICDD106 pKa = 4.37 SMMNSTDD113 pKa = 3.04 RR114 pKa = 11.84 FLRR117 pKa = 11.84 SSPAWLPIVSQLYY130 pKa = 10.2 ISVLWNVMIIRR141 pKa = 11.84 ILSHH145 pKa = 6.05 TGYY148 pKa = 10.85 APSFTDD154 pKa = 4.84 LLNTLTTDD162 pKa = 3.96 LQIEE166 pKa = 4.57 EE167 pKa = 4.74 CMVPGPLVPFFQSLASINGPFDD189 pKa = 4.03 WIGDD193 pKa = 3.7 ITPALPGFDD202 pKa = 4.3 SLWDD206 pKa = 3.52 ATNFTPHH213 pKa = 6.4 ASYY216 pKa = 10.46 TRR218 pKa = 11.84 FVPIPAILLDD228 pKa = 3.47 QLYY231 pKa = 10.07 YY232 pKa = 9.99 CATYY236 pKa = 10.89 VGVNQGDD243 pKa = 4.75 LYY245 pKa = 10.28 PTFTWYY251 pKa = 10.88 RR252 pKa = 11.84 NIFTRR257 pKa = 11.84 TGANTPALLRR267 pKa = 11.84 MGPNLCGSLFTTSNQFDD284 pKa = 3.55 AARR287 pKa = 11.84 TYY289 pKa = 9.62 WRR291 pKa = 11.84 ACFGTGFTRR300 pKa = 11.84 VNVTAAAFTNYY311 pKa = 9.88 LQLLGLRR318 pKa = 11.84 SQTGEE323 pKa = 3.9 PQTAWFQNVTMVMQKK338 pKa = 10.16 YY339 pKa = 8.49 AQHH342 pKa = 6.55 FNGSVPLKK350 pKa = 10.8 SIDD353 pKa = 3.47 LTGIGAVALTGTPVNNTAVRR373 pKa = 11.84 DD374 pKa = 3.58 WLYY377 pKa = 10.5 PPNANIEE384 pKa = 4.18 PFTTGRR390 pKa = 11.84 FNPRR394 pKa = 11.84 RR395 pKa = 11.84 EE396 pKa = 3.76 IPAQLRR402 pKa = 11.84 IRR404 pKa = 11.84 FSHH407 pKa = 6.63 SDD409 pKa = 3.38 HH410 pKa = 6.46 EE411 pKa = 4.78 LEE413 pKa = 4.97 LQAEE417 pKa = 4.5 QYY419 pKa = 11.17 AIAAHH424 pKa = 5.61 TNIRR428 pKa = 11.84 WAANIATQHH437 pKa = 5.67 EE438 pKa = 4.36 RR439 pKa = 11.84 TAINPAHH446 pKa = 6.87 LHH448 pKa = 6.17 QGDD451 pKa = 4.3 YY452 pKa = 11.31 WNMTPFRR459 pKa = 11.84 HH460 pKa = 5.94 TGHH463 pKa = 7.36 LGLKK467 pKa = 6.8 TQYY470 pKa = 10.6 AQLIASRR477 pKa = 11.84 YY478 pKa = 7.81 HH479 pKa = 5.22 QLAANRR485 pKa = 11.84 VDD487 pKa = 3.38
Molecular weight: 53.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.53
IPC2_protein 6.465
IPC_protein 6.634
Toseland 6.883
ProMoST 6.883
Dawson 7.044
Bjellqvist 6.971
Wikipedia 7.059
Rodwell 7.044
Grimsley 7.029
Solomon 7.073
Lehninger 7.073
Nozaki 7.293
DTASelect 7.439
Thurlkill 7.468
EMBOSS 7.468
Sillero 7.439
Patrickios 3.719
IPC_peptide 7.073
IPC2_peptide 7.307
IPC2.peptide.svr19 7.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1103
487
616
551.5
63.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.978 ± 2.193
1.179 ± 0.1
4.986 ± 0.853
4.261 ± 1.053
6.346 ± 0.802
5.077 ± 0.445
3.808 ± 0.345
6.528 ± 0.379
2.539 ± 1.136
8.704 ± 0.188
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.539 ± 0.049
5.712 ± 0.297
7.072 ± 0.619
3.354 ± 1.176
6.256 ± 0.606
4.986 ± 0.64
8.069 ± 0.639
3.717 ± 0.258
2.448 ± 0.261
4.442 ± 0.494
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here