Pelagibacter phage HTVC019P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; Pelagibacter virus HTVC019P

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1I8C0|M1I8C0_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC019P OX=1283079 PE=4 SV=1
MM1 pKa = 7.99AINLANNNSLANITALPSSISGGGMTLISEE31 pKa = 4.43QTASSSATIDD41 pKa = 3.51FTSGIDD47 pKa = 3.53DD48 pKa = 4.16TYY50 pKa = 11.64DD51 pKa = 3.21SYY53 pKa = 12.22VFKK56 pKa = 10.56FYY58 pKa = 10.7NIHH61 pKa = 7.02PATDD65 pKa = 3.27SANFQVNFRR74 pKa = 11.84DD75 pKa = 3.63GGTDD79 pKa = 3.14YY80 pKa = 11.55DD81 pKa = 3.98AVKK84 pKa = 8.42TTTAFFAFHH93 pKa = 5.85QASNGSTNLAYY104 pKa = 9.74STASDD109 pKa = 4.29LAQSTGFFKK118 pKa = 10.8IMTTMGNDD126 pKa = 3.23NEE128 pKa = 4.66GCGVGTLRR136 pKa = 11.84IFNPSSTTFVKK147 pKa = 10.6HH148 pKa = 6.75FMSDD152 pKa = 3.42CNNWHH157 pKa = 6.81SNDD160 pKa = 3.61SNATVNWRR168 pKa = 11.84AAGYY172 pKa = 10.6CNTTNVIDD180 pKa = 4.16GAQFKK185 pKa = 10.3FDD187 pKa = 4.16SGNIDD192 pKa = 3.22SGVIKK197 pKa = 10.68LYY199 pKa = 10.95GISS202 pKa = 3.35

Molecular weight: 21.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1IDV8|M1IDV8_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC019P OX=1283079 PE=4 SV=1
MM1 pKa = 7.51LSSFKK6 pKa = 10.88LSLRR10 pKa = 11.84RR11 pKa = 11.84FLYY14 pKa = 10.35NFHH17 pKa = 7.88LMFTAKK23 pKa = 9.54RR24 pKa = 11.84DD25 pKa = 3.56YY26 pKa = 10.32EE27 pKa = 4.21KK28 pKa = 10.56QVRR31 pKa = 11.84LIVKK35 pKa = 9.51YY36 pKa = 10.88DD37 pKa = 3.59PLNILNN43 pKa = 4.12

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13098

40

1265

222.0

24.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.673 ± 0.333

0.863 ± 0.133

6.031 ± 0.169

6.329 ± 0.481

3.97 ± 0.219

6.406 ± 0.319

1.703 ± 0.165

6.627 ± 0.238

8.276 ± 0.501

8.024 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.235

6.115 ± 0.333

2.863 ± 0.185

3.726 ± 0.207

3.764 ± 0.254

7.177 ± 0.418

7.413 ± 0.529

5.795 ± 0.359

1.069 ± 0.149

3.504 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski