Roseovarius atlanticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T5NP35|A0A0T5NP35_9RHOB Phosphoribosylaminoimidazole-succinocarboxamide synthase OS=Roseovarius atlanticus OX=1641875 GN=purC PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 10.38RR3 pKa = 11.84ATPLLAAAFALGAAPAFAGNLTPVQDD29 pKa = 4.58DD30 pKa = 4.14VVAPPPPPPVAATPNWTGLYY50 pKa = 10.55GGVQLGYY57 pKa = 11.28NNLDD61 pKa = 3.8SNISGGDD68 pKa = 3.23EE69 pKa = 4.06TVIGGLFVGYY79 pKa = 10.28DD80 pKa = 3.4YY81 pKa = 11.77DD82 pKa = 3.73FGNFVLGSSLDD93 pKa = 3.73YY94 pKa = 11.33DD95 pKa = 3.74FTEE98 pKa = 5.36ADD100 pKa = 3.55VSNAPNPQVDD110 pKa = 4.03LEE112 pKa = 4.49NIFRR116 pKa = 11.84AKK118 pKa = 10.17LRR120 pKa = 11.84AGYY123 pKa = 10.38KK124 pKa = 10.03LGNGLIYY131 pKa = 9.5GTGGYY136 pKa = 10.62AKK138 pKa = 10.64AFTDD142 pKa = 4.67NLGDD146 pKa = 3.94DD147 pKa = 3.98DD148 pKa = 5.83GYY150 pKa = 11.5FVGAGYY156 pKa = 8.34EE157 pKa = 4.25TMVTDD162 pKa = 3.79NFSLGGEE169 pKa = 4.16LLYY172 pKa = 11.07HH173 pKa = 6.61EE174 pKa = 5.43FDD176 pKa = 3.97NFNGSNADD184 pKa = 3.62VEE186 pKa = 4.53PTTVQLRR193 pKa = 11.84GTFRR197 pKa = 11.84FF198 pKa = 3.79

Molecular weight: 20.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T5NVC6|A0A0T5NVC6_9RHOB Stress responsive protein OS=Roseovarius atlanticus OX=1641875 GN=XM53_10020 PE=4 SV=1
MM1 pKa = 7.59PRR3 pKa = 11.84TRR5 pKa = 11.84SPHH8 pKa = 4.88SLRR11 pKa = 11.84NRR13 pKa = 11.84LKK15 pKa = 10.01RR16 pKa = 11.84ALVRR20 pKa = 11.84IRR22 pKa = 11.84SRR24 pKa = 11.84VPPGLRR30 pKa = 11.84LVLGILLIIGGIFGFLPVLGFWMIPLGIAVASLDD64 pKa = 4.1VVPLWRR70 pKa = 11.84RR71 pKa = 11.84LTGRR75 pKa = 11.84LPKK78 pKa = 10.28RR79 pKa = 3.59

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4236

0

4236

1284231

32

2891

303.2

32.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.067 ± 0.048

0.939 ± 0.013

6.295 ± 0.031

6.163 ± 0.04

3.715 ± 0.024

8.752 ± 0.038

2.084 ± 0.02

5.063 ± 0.028

3.144 ± 0.035

9.883 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.832 ± 0.02

2.531 ± 0.019

5.057 ± 0.026

3.139 ± 0.019

6.733 ± 0.033

5.022 ± 0.027

5.581 ± 0.03

7.341 ± 0.039

1.405 ± 0.015

2.253 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski