Circovirus-like genome DHCV-1
Average proteome isoelectric point is 8.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A190WHM5|A0A190WHM5_9CIRC ATP-dependent helicase Rep OS=Circovirus-like genome DHCV-1 OX=1788450 PE=3 SV=1
MM1 pKa = 7.2 KK2 pKa = 10.55 VKK4 pKa = 10.62 VYY6 pKa = 11.08 TNFKK10 pKa = 8.13 SKK12 pKa = 10.66 KK13 pKa = 8.6 VIRR16 pKa = 11.84 AKK18 pKa = 11.0 YY19 pKa = 10.24 SITLALTWITFSFPSHH35 pKa = 6.29 PTSNMSYY42 pKa = 9.82 QLNWCFTVNNYY53 pKa = 7.67 TVLDD57 pKa = 4.02 EE58 pKa = 5.6 QKK60 pKa = 10.66 LQDD63 pKa = 3.8 MPYY66 pKa = 10.17 KK67 pKa = 9.26 YY68 pKa = 10.26 LIYY71 pKa = 10.08 GRR73 pKa = 11.84 EE74 pKa = 4.07 VGEE77 pKa = 4.33 EE78 pKa = 4.28 GTPHH82 pKa = 5.73 LQGYY86 pKa = 7.1 VQYY89 pKa = 10.6 KK90 pKa = 9.6 KK91 pKa = 10.74 KK92 pKa = 10.71 LRR94 pKa = 11.84 LTALKK99 pKa = 10.03 KK100 pKa = 9.8 LHH102 pKa = 6.44 PTAHH106 pKa = 6.36 WEE108 pKa = 3.77 AAKK111 pKa = 10.93 GNEE114 pKa = 4.33 EE115 pKa = 4.02 QNKK118 pKa = 8.4 TYY120 pKa = 10.33 CSKK123 pKa = 11.05 EE124 pKa = 3.55 GDD126 pKa = 3.61 FLEE129 pKa = 5.34 FGTPTKK135 pKa = 10.48 TNGGASTIAARR146 pKa = 11.84 AEE148 pKa = 3.93 KK149 pKa = 10.19 NARR152 pKa = 11.84 LMTAPIEE159 pKa = 3.98 EE160 pKa = 5.28 LILTGEE166 pKa = 4.61 LHH168 pKa = 6.14 PQHH171 pKa = 6.87 AKK173 pKa = 10.28 AIHH176 pKa = 5.67 NARR179 pKa = 11.84 ILVQEE184 pKa = 4.72 EE185 pKa = 3.95 RR186 pKa = 11.84 RR187 pKa = 11.84 KK188 pKa = 10.11 LHH190 pKa = 6.75 PPDD193 pKa = 5.29 ALDD196 pKa = 4.52 GDD198 pKa = 5.09 LPHH201 pKa = 6.83 LWFWGPAGTGKK212 pKa = 10.24 SYY214 pKa = 10.93 KK215 pKa = 10.45 ARR217 pKa = 11.84 TDD219 pKa = 3.39 YY220 pKa = 11.24 PDD222 pKa = 4.75 AYY224 pKa = 10.92 LKK226 pKa = 10.62 AANKK230 pKa = 7.73 WWCGYY235 pKa = 9.96 NDD237 pKa = 4.11 EE238 pKa = 4.47 DD239 pKa = 4.57 TVLIEE244 pKa = 4.49 EE245 pKa = 4.21 WDD247 pKa = 3.68 KK248 pKa = 11.27 NHH250 pKa = 6.45 NVLAHH255 pKa = 6.37 HH256 pKa = 6.89 LKK258 pKa = 10.54 LWADD262 pKa = 4.38 RR263 pKa = 11.84 YY264 pKa = 9.66 PFSGEE269 pKa = 4.04 VKK271 pKa = 10.46 GGTTGKK277 pKa = 9.58 IRR279 pKa = 11.84 PKK281 pKa = 10.33 RR282 pKa = 11.84 IVITSNYY289 pKa = 8.7 HH290 pKa = 6.51 PRR292 pKa = 11.84 DD293 pKa = 2.92 IWGDD297 pKa = 3.73 DD298 pKa = 3.48 PEE300 pKa = 5.67 GALGPIMRR308 pKa = 11.84 RR309 pKa = 11.84 FNVVHH314 pKa = 7.04 FDD316 pKa = 3.43 QKK318 pKa = 11.56 FNFF321 pKa = 3.83
Molecular weight: 36.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.617
IPC2_protein 8.565
IPC_protein 8.463
Toseland 9.121
ProMoST 8.946
Dawson 9.428
Bjellqvist 9.194
Wikipedia 9.604
Rodwell 9.663
Grimsley 9.502
Solomon 9.487
Lehninger 9.443
Nozaki 9.253
DTASelect 9.151
Thurlkill 9.282
EMBOSS 9.575
Sillero 9.399
Patrickios 4.635
IPC_peptide 9.472
IPC2_peptide 7.761
IPC2.peptide.svr19 7.676
Protein with the highest isoelectric point:
>tr|A0A190WHM5|A0A190WHM5_9CIRC ATP-dependent helicase Rep OS=Circovirus-like genome DHCV-1 OX=1788450 PE=3 SV=1
MM1 pKa = 7.2 KK2 pKa = 10.55 VKK4 pKa = 10.62 VYY6 pKa = 11.08 TNFKK10 pKa = 8.13 SKK12 pKa = 10.66 KK13 pKa = 8.6 VIRR16 pKa = 11.84 AKK18 pKa = 11.0 YY19 pKa = 10.24 SITLALTWITFSFPSHH35 pKa = 6.29 PTSNMSYY42 pKa = 9.82 QLNWCFTVNNYY53 pKa = 7.67 TVLDD57 pKa = 4.02 EE58 pKa = 5.6 QKK60 pKa = 10.66 LQDD63 pKa = 3.8 MPYY66 pKa = 10.17 KK67 pKa = 9.26 YY68 pKa = 10.26 LIYY71 pKa = 10.08 GRR73 pKa = 11.84 EE74 pKa = 4.07 VGEE77 pKa = 4.33 EE78 pKa = 4.28 GTPHH82 pKa = 5.73 LQGYY86 pKa = 7.1 VQYY89 pKa = 10.6 KK90 pKa = 9.6 KK91 pKa = 10.74 KK92 pKa = 10.71 LRR94 pKa = 11.84 LTALKK99 pKa = 10.03 KK100 pKa = 9.8 LHH102 pKa = 6.44 PTAHH106 pKa = 6.36 WEE108 pKa = 3.77 AAKK111 pKa = 10.93 GNEE114 pKa = 4.33 EE115 pKa = 4.02 QNKK118 pKa = 8.4 TYY120 pKa = 10.33 CSKK123 pKa = 11.05 EE124 pKa = 3.55 GDD126 pKa = 3.61 FLEE129 pKa = 5.34 FGTPTKK135 pKa = 10.48 TNGGASTIAARR146 pKa = 11.84 AEE148 pKa = 3.93 KK149 pKa = 10.19 NARR152 pKa = 11.84 LMTAPIEE159 pKa = 3.98 EE160 pKa = 5.28 LILTGEE166 pKa = 4.61 LHH168 pKa = 6.14 PQHH171 pKa = 6.87 AKK173 pKa = 10.28 AIHH176 pKa = 5.67 NARR179 pKa = 11.84 ILVQEE184 pKa = 4.72 EE185 pKa = 3.95 RR186 pKa = 11.84 RR187 pKa = 11.84 KK188 pKa = 10.11 LHH190 pKa = 6.75 PPDD193 pKa = 5.29 ALDD196 pKa = 4.52 GDD198 pKa = 5.09 LPHH201 pKa = 6.83 LWFWGPAGTGKK212 pKa = 10.24 SYY214 pKa = 10.93 KK215 pKa = 10.45 ARR217 pKa = 11.84 TDD219 pKa = 3.39 YY220 pKa = 11.24 PDD222 pKa = 4.75 AYY224 pKa = 10.92 LKK226 pKa = 10.62 AANKK230 pKa = 7.73 WWCGYY235 pKa = 9.96 NDD237 pKa = 4.11 EE238 pKa = 4.47 DD239 pKa = 4.57 TVLIEE244 pKa = 4.49 EE245 pKa = 4.21 WDD247 pKa = 3.68 KK248 pKa = 11.27 NHH250 pKa = 6.45 NVLAHH255 pKa = 6.37 HH256 pKa = 6.89 LKK258 pKa = 10.54 LWADD262 pKa = 4.38 RR263 pKa = 11.84 YY264 pKa = 9.66 PFSGEE269 pKa = 4.04 VKK271 pKa = 10.46 GGTTGKK277 pKa = 9.58 IRR279 pKa = 11.84 PKK281 pKa = 10.33 RR282 pKa = 11.84 IVITSNYY289 pKa = 8.7 HH290 pKa = 6.51 PRR292 pKa = 11.84 DD293 pKa = 2.92 IWGDD297 pKa = 3.73 DD298 pKa = 3.48 PEE300 pKa = 5.67 GALGPIMRR308 pKa = 11.84 RR309 pKa = 11.84 FNVVHH314 pKa = 7.04 FDD316 pKa = 3.43 QKK318 pKa = 11.56 FNFF321 pKa = 3.83
Molecular weight: 36.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.617
IPC2_protein 8.565
IPC_protein 8.463
Toseland 9.121
ProMoST 8.946
Dawson 9.428
Bjellqvist 9.194
Wikipedia 9.604
Rodwell 9.663
Grimsley 9.502
Solomon 9.487
Lehninger 9.443
Nozaki 9.253
DTASelect 9.151
Thurlkill 9.282
EMBOSS 9.575
Sillero 9.399
Patrickios 4.635
IPC_peptide 9.472
IPC2_peptide 7.761
IPC2.peptide.svr19 7.676
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
321
321
321
321.0
36.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.477 ± 0.0
0.935 ± 0.0
4.984 ± 0.0
6.231 ± 0.0
3.738 ± 0.0
6.854 ± 0.0
4.361 ± 0.0
4.673 ± 0.0
9.346 ± 0.0
8.411 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.558 ± 0.0
5.296 ± 0.0
5.607 ± 0.0
2.804 ± 0.0
4.673 ± 0.0
3.427 ± 0.0
7.165 ± 0.0
4.361 ± 0.0
3.115 ± 0.0
4.984 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here