Azobacteroides phage ProJPt-Bp1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V1FGX9|A0A1V1FGX9_9CAUD VIP2 Actin-ADP-ribosylating toxin family protein OS=Azobacteroides phage ProJPt-Bp1 OX=1920526 PE=4 SV=1
MM1 pKa = 7.26AQHH4 pKa = 6.8IDD6 pKa = 3.34MYY8 pKa = 10.29DD9 pKa = 3.06IRR11 pKa = 11.84IGPDD15 pKa = 2.63KK16 pKa = 10.82FIIIDD21 pKa = 3.43NHH23 pKa = 5.9ALYY26 pKa = 8.04TTFSIEE32 pKa = 3.86FEE34 pKa = 4.04IEE36 pKa = 3.23EE37 pKa = 5.05GYY39 pKa = 10.08IQFTDD44 pKa = 3.78DD45 pKa = 4.07LVGDD49 pKa = 3.97VYY51 pKa = 10.78IGQIANMTIDD61 pKa = 3.74GVAAATTGEE70 pKa = 4.18EE71 pKa = 4.69LADD74 pKa = 3.63QLGDD78 pKa = 3.56YY79 pKa = 7.56MTDD82 pKa = 3.07VLSEE86 pKa = 4.08ADD88 pKa = 3.81IEE90 pKa = 4.67TSGATQYY97 pKa = 11.78DD98 pKa = 4.0FVDD101 pKa = 4.66DD102 pKa = 4.08GLGFQVKK109 pKa = 10.01HH110 pKa = 6.12EE111 pKa = 4.12NSVVYY116 pKa = 10.17DD117 pKa = 3.7YY118 pKa = 11.52SANKK122 pKa = 9.22LVPVTSVSTTTGTGRR137 pKa = 11.84INNLTTGIMISHH149 pKa = 7.66TGTSYY154 pKa = 10.68IPSLTIHH161 pKa = 6.31SGDD164 pKa = 3.43PAMLFVFKK172 pKa = 10.83NGTSTLVALSSDD184 pKa = 3.38GTLATTKK191 pKa = 9.97SQNYY195 pKa = 6.93NTMPNGEE202 pKa = 4.57YY203 pKa = 8.73VTKK206 pKa = 10.82ALADD210 pKa = 3.5TAYY213 pKa = 10.73VDD215 pKa = 3.85TFASLVGGSFTVTRR229 pKa = 11.84RR230 pKa = 11.84GNIIFNYY237 pKa = 10.03SAPQWEE243 pKa = 4.58YY244 pKa = 10.77TFNNLSGGFVVAKK257 pKa = 9.36NGTVIYY263 pKa = 8.78TYY265 pKa = 9.66TPPVVPDD272 pKa = 3.05QDD274 pKa = 3.79VYY276 pKa = 11.56TFADD280 pKa = 3.89YY281 pKa = 7.6PTTSGFQVSKK291 pKa = 10.81NSTPIYY297 pKa = 9.46TYY299 pKa = 10.98SAVLDD304 pKa = 4.05TFTDD308 pKa = 3.42EE309 pKa = 5.35DD310 pKa = 4.4DD311 pKa = 3.94GFKK314 pKa = 10.43VARR317 pKa = 11.84GGSTIYY323 pKa = 10.24TYY325 pKa = 10.16TPPDD329 pKa = 3.47SAVDD333 pKa = 3.71TFSDD337 pKa = 4.0NDD339 pKa = 3.7DD340 pKa = 4.64DD341 pKa = 4.47GFKK344 pKa = 9.59VTRR347 pKa = 11.84DD348 pKa = 3.21GQVIYY353 pKa = 10.63NYY355 pKa = 10.12DD356 pKa = 3.56PPIVGGSLDD365 pKa = 4.11FEE367 pKa = 4.88TTTDD371 pKa = 2.81QYY373 pKa = 11.94GFVTGFEE380 pKa = 4.43VKK382 pKa = 10.62DD383 pKa = 3.75EE384 pKa = 4.21EE385 pKa = 5.53DD386 pKa = 3.57EE387 pKa = 4.37VLYY390 pKa = 10.11TYY392 pKa = 11.67ADD394 pKa = 3.47AQYY397 pKa = 10.54GAQTIGTAMNLYY409 pKa = 10.52KK410 pKa = 10.43SGQLVYY416 pKa = 9.7TYY418 pKa = 10.7DD419 pKa = 3.13AAAIVGSGFARR430 pKa = 11.84SIIKK434 pKa = 10.1SDD436 pKa = 3.6SGDD439 pKa = 3.65EE440 pKa = 3.97EE441 pKa = 5.8LMISQIADD449 pKa = 3.39EE450 pKa = 4.44VGGYY454 pKa = 8.61TMALLQSSTIGGGGKK469 pKa = 8.23RR470 pKa = 11.84TITT473 pKa = 3.49

Molecular weight: 51.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V1FY48|A0A1V1FY48_9CAUD Phage protein inside capsid D OS=Azobacteroides phage ProJPt-Bp1 OX=1920526 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.1PFYY5 pKa = 10.76NYY7 pKa = 9.12TDD9 pKa = 3.43PRR11 pKa = 11.84DD12 pKa = 3.44NRR14 pKa = 11.84FVFKK18 pKa = 10.67GSEE21 pKa = 3.89NRR23 pKa = 11.84PSAVAMEE30 pKa = 5.13DD31 pKa = 2.93IVRR34 pKa = 11.84GAKK37 pKa = 9.98NNPTFIDD44 pKa = 4.05FQEE47 pKa = 4.09RR48 pKa = 11.84QYY50 pKa = 11.42PGTKK54 pKa = 9.3KK55 pKa = 10.52AFRR58 pKa = 11.84KK59 pKa = 8.42WLAYY63 pKa = 10.17DD64 pKa = 3.85PQRR67 pKa = 11.84MIDD70 pKa = 3.61PATNMPYY77 pKa = 10.95DD78 pKa = 4.01LAAWNVPTEE87 pKa = 4.34MPSDD91 pKa = 3.74TPIDD95 pKa = 3.92FLSPTAKK102 pKa = 10.32HH103 pKa = 5.99GGSVQRR109 pKa = 11.84FAHH112 pKa = 6.24GGSISGLDD120 pKa = 3.4GFKK123 pKa = 11.05YY124 pKa = 10.24PIRR127 pKa = 11.84RR128 pKa = 11.84YY129 pKa = 8.86ATGGSAAVPASGTLEE144 pKa = 4.48AIAAASAAADD154 pKa = 3.45KK155 pKa = 9.91ATAKK159 pKa = 10.31GGSWGDD165 pKa = 3.69FANTAAQTVNSSGVSISDD183 pKa = 3.57MIRR186 pKa = 11.84NLFRR190 pKa = 11.84KK191 pKa = 9.92RR192 pKa = 11.84PTVGANDD199 pKa = 3.76YY200 pKa = 9.2YY201 pKa = 10.54YY202 pKa = 11.22KK203 pKa = 11.07VMEE206 pKa = 4.15EE207 pKa = 3.99ALRR210 pKa = 11.84GSLAKK215 pKa = 10.35AQSGRR220 pKa = 11.84GADD223 pKa = 4.07LLPPTKK229 pKa = 10.1PQDD232 pKa = 3.21IEE234 pKa = 4.31AEE236 pKa = 4.02PDD238 pKa = 2.84IDD240 pKa = 3.81PAYY243 pKa = 7.31MTSEE247 pKa = 4.41RR248 pKa = 11.84NNMTGVVAKK257 pKa = 10.94ALMDD261 pKa = 4.54KK262 pKa = 10.8IKK264 pKa = 10.37DD265 pKa = 3.66TDD267 pKa = 3.86SEE269 pKa = 4.51RR270 pKa = 11.84SDD272 pKa = 3.33ALEE275 pKa = 3.93RR276 pKa = 11.84LASRR280 pKa = 11.84RR281 pKa = 11.84RR282 pKa = 11.84EE283 pKa = 3.78AGRR286 pKa = 11.84AGDD289 pKa = 3.68APTRR293 pKa = 11.84LAMILEE299 pKa = 4.39AQRR302 pKa = 11.84QYY304 pKa = 11.16GNEE307 pKa = 4.01MKK309 pKa = 10.55GARR312 pKa = 11.84AAADD316 pKa = 3.21ASNRR320 pKa = 11.84DD321 pKa = 3.12TRR323 pKa = 11.84AEE325 pKa = 4.12FARR328 pKa = 11.84TMAAIGDD335 pKa = 4.07ADD337 pKa = 3.63SAKK340 pKa = 10.75AMEE343 pKa = 4.53ASRR346 pKa = 11.84HH347 pKa = 5.13NIEE350 pKa = 3.91EE351 pKa = 4.14KK352 pKa = 10.46TRR354 pKa = 11.84VDD356 pKa = 3.4DD357 pKa = 4.41LNARR361 pKa = 11.84RR362 pKa = 11.84HH363 pKa = 4.95AAHH366 pKa = 6.4RR367 pKa = 11.84QARR370 pKa = 11.84SAQNYY375 pKa = 9.73AYY377 pKa = 8.93EE378 pKa = 4.06QEE380 pKa = 4.2KK381 pKa = 10.56RR382 pKa = 11.84AFNNQQRR389 pKa = 11.84MDD391 pKa = 3.42EE392 pKa = 4.21YY393 pKa = 11.33NRR395 pKa = 11.84SKK397 pKa = 11.01EE398 pKa = 3.92SDD400 pKa = 3.2ALRR403 pKa = 11.84LQLAEE408 pKa = 3.66LHH410 pKa = 5.79GRR412 pKa = 11.84RR413 pKa = 11.84GQEE416 pKa = 3.15ADD418 pKa = 3.73LAKK421 pKa = 10.81EE422 pKa = 3.77MAKK425 pKa = 10.23EE426 pKa = 3.92NRR428 pKa = 11.84WKK430 pKa = 10.39SWRR433 pKa = 11.84TWLKK437 pKa = 10.45LLGMGAGATIGGMTGGVAGAGLGLSLGSVLKK468 pKa = 10.7HH469 pKa = 5.6GGSVRR474 pKa = 11.84RR475 pKa = 11.84YY476 pKa = 10.37SKK478 pKa = 11.35GGMQNAWMNQGKK490 pKa = 10.19DD491 pKa = 2.47IDD493 pKa = 4.31LYY495 pKa = 11.72VRR497 pKa = 11.84TKK499 pKa = 10.19KK500 pKa = 10.15QRR502 pKa = 11.84NPFRR506 pKa = 5.79

Molecular weight: 55.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

31065

102

3481

597.4

67.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.207 ± 0.336

0.592 ± 0.127

6.992 ± 0.202

6.103 ± 0.243

3.827 ± 0.18

7.574 ± 0.399

2.002 ± 0.12

5.765 ± 0.307

6.193 ± 0.34

6.921 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.129 ± 0.147

5.521 ± 0.166

5.453 ± 0.424

4.214 ± 0.389

4.948 ± 0.291

5.981 ± 0.251

6.158 ± 0.379

5.592 ± 0.289

1.336 ± 0.068

4.491 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski