Soybean dwarf virus
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91QR2|Q91QR2_9LUTE Movement protein OS=Soybean dwarf virus OX=12049 GN=ORF4 PE=3 SV=1
VV1 pKa = 7.46 DD2 pKa = 4.87 GEE4 pKa = 4.6 PGPKK8 pKa = 9.42 PGPDD12 pKa = 3.72 PAPQPTPTPEE22 pKa = 3.71 PTPAKK27 pKa = 9.75 HH28 pKa = 6.09 EE29 pKa = 4.09 RR30 pKa = 11.84 FIAYY34 pKa = 7.69 TGTLSTLISARR45 pKa = 11.84 QSSDD49 pKa = 3.46 SISLYY54 pKa = 10.01 SIRR57 pKa = 11.84 SQRR60 pKa = 11.84 IRR62 pKa = 11.84 YY63 pKa = 9.1 IEE65 pKa = 4.77 DD66 pKa = 3.59 EE67 pKa = 4.13 NSSWTNIDD75 pKa = 3.62 AKK77 pKa = 10.16 WYY79 pKa = 8.63 SQNSVEE85 pKa = 4.73 AIPMFVYY92 pKa = 9.48 PVPEE96 pKa = 4.3 GTWSIEE102 pKa = 3.88 ISCEE106 pKa = 3.95 GYY108 pKa = 9.81 QAASSTSDD116 pKa = 3.17 PHH118 pKa = 7.88 RR119 pKa = 11.84 GKK121 pKa = 10.64 CDD123 pKa = 2.94 GMIAYY128 pKa = 9.88 DD129 pKa = 4.3 DD130 pKa = 4.93 DD131 pKa = 4.88 SSKK134 pKa = 10.55 VWNVGQQNNVTITNNKK150 pKa = 9.17 ADD152 pKa = 4.07 NDD154 pKa = 4.0 WKK156 pKa = 11.0 YY157 pKa = 11.15 GHH159 pKa = 7.75 PDD161 pKa = 3.01 LTINGDD167 pKa = 3.54 RR168 pKa = 11.84 FDD170 pKa = 3.77 QNQVVEE176 pKa = 4.13 KK177 pKa = 10.71 DD178 pKa = 3.98 GIISFHH184 pKa = 6.69 LVTTGPNASFFLVAPAVKK202 pKa = 8.86 KK203 pKa = 6.9 TAKK206 pKa = 10.58 YY207 pKa = 9.35 NFCVSYY213 pKa = 11.32 GDD215 pKa = 3.19 WTDD218 pKa = 3.56 RR219 pKa = 11.84 DD220 pKa = 3.87 MEE222 pKa = 4.49 FGMVSVVLDD231 pKa = 3.47 EE232 pKa = 4.66 HH233 pKa = 8.3 LEE235 pKa = 4.22 GARR238 pKa = 11.84 SSQYY242 pKa = 9.71 VRR244 pKa = 11.84 KK245 pKa = 7.62 TPRR248 pKa = 11.84 SGHH251 pKa = 5.3 VGVNRR256 pKa = 11.84 SHH258 pKa = 7.25 RR259 pKa = 11.84 LQDD262 pKa = 3.19 NFVPTEE268 pKa = 3.97 YY269 pKa = 11.14 VSDD272 pKa = 3.89 EE273 pKa = 4.39 DD274 pKa = 4.06 SSSNSSIVSNRR285 pKa = 11.84 PSTPDD290 pKa = 3.17 NDD292 pKa = 3.69 SDD294 pKa = 4.09 AKK296 pKa = 10.53 FAEE299 pKa = 4.34 SMKK302 pKa = 10.83 GKK304 pKa = 10.29 LPSQTKK310 pKa = 10.17 LPPKK314 pKa = 10.43 GFLSQLSTKK323 pKa = 8.39 EE324 pKa = 3.74 KK325 pKa = 11.0 KK326 pKa = 10.13 EE327 pKa = 3.72 ISNSKK332 pKa = 8.98 PSNVEE337 pKa = 3.79 GLVGPLVAAYY347 pKa = 8.65 GYY349 pKa = 9.02 PSQTGVHH356 pKa = 6.36 DD357 pKa = 4.11 AARR360 pKa = 11.84 EE361 pKa = 3.71 ILQAKK366 pKa = 8.14 EE367 pKa = 3.51 AAEE370 pKa = 3.85 NLAEE374 pKa = 4.64 LEE376 pKa = 4.14 RR377 pKa = 11.84 DD378 pKa = 3.2 LKK380 pKa = 10.8 EE381 pKa = 4.13 INKK384 pKa = 9.88 LEE386 pKa = 4.37 PPDD389 pKa = 4.53 VIVQEE394 pKa = 4.66 EE395 pKa = 4.43 IPDD398 pKa = 3.87 FVPPSEE404 pKa = 5.26 KK405 pKa = 9.61 ILKK408 pKa = 9.96 EE409 pKa = 4.11 DD410 pKa = 4.1 DD411 pKa = 3.75 PDD413 pKa = 3.72 YY414 pKa = 11.5 VPPIWHH420 pKa = 6.66 NADD423 pKa = 3.08 QAVLVSSYY431 pKa = 10.73 EE432 pKa = 3.88 PPDD435 pKa = 3.33 WSRR438 pKa = 11.84 PAYY441 pKa = 10.0 EE442 pKa = 5.22 SGDD445 pKa = 3.8 PPKK448 pKa = 10.29 KK449 pKa = 9.24 TGTLKK454 pKa = 10.1 GTLSKK459 pKa = 11.03 LGGSLRR465 pKa = 11.84 SGEE468 pKa = 4.1 SSLRR472 pKa = 11.84 GSLRR476 pKa = 11.84 KK477 pKa = 7.81 TQDD480 pKa = 2.88 QTDD483 pKa = 4.12 LDD485 pKa = 4.08 NKK487 pKa = 10.25 LSKK490 pKa = 10.77 LSVIQRR496 pKa = 11.84 SRR498 pKa = 11.84 YY499 pKa = 8.05 QRR501 pKa = 11.84 ILNNLGKK508 pKa = 9.24 MRR510 pKa = 11.84 ARR512 pKa = 11.84 TYY514 pKa = 10.81 IDD516 pKa = 4.79 GLDD519 pKa = 3.54 LDD521 pKa = 4.29
Molecular weight: 57.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.066
IPC2_protein 5.08
IPC_protein 5.041
Toseland 4.965
ProMoST 5.143
Dawson 5.041
Bjellqvist 5.181
Wikipedia 4.927
Rodwell 4.94
Grimsley 4.889
Solomon 5.041
Lehninger 5.003
Nozaki 5.156
DTASelect 5.347
Thurlkill 4.978
EMBOSS 4.965
Sillero 5.232
Patrickios 4.202
IPC_peptide 5.054
IPC2_peptide 5.219
IPC2.peptide.svr19 5.219
Protein with the highest isoelectric point:
>tr|Q91QR4|Q91QR4_9LUTE RNA-directed RNA polymerase (Fragment) OS=Soybean dwarf virus OX=12049 GN=ORF2 PE=4 SV=1
MM1 pKa = 6.96 VAVSNVAIQRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 TRR15 pKa = 11.84 RR16 pKa = 11.84 AARR19 pKa = 11.84 RR20 pKa = 11.84 APRR23 pKa = 11.84 VQLMAVPTVTSRR35 pKa = 11.84 PQRR38 pKa = 11.84 RR39 pKa = 11.84 GRR41 pKa = 11.84 QRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 NNRR50 pKa = 11.84 GGSFISGGSGKK61 pKa = 10.11 AHH63 pKa = 5.8 TFVFSKK69 pKa = 10.92 DD70 pKa = 3.62 GINGSSKK77 pKa = 10.83 GSITFGPSLSEE88 pKa = 4.21 CKK90 pKa = 10.0 PFSDD94 pKa = 5.84 GILKK98 pKa = 10.28 AYY100 pKa = 10.09 HH101 pKa = 6.59 EE102 pKa = 4.33 YY103 pKa = 10.69 KK104 pKa = 9.21 ITSILLQFITEE115 pKa = 4.23 ASSTSSGSIAYY126 pKa = 9.43 EE127 pKa = 3.79 LDD129 pKa = 3.32 PHH131 pKa = 6.86 CKK133 pKa = 9.64 YY134 pKa = 11.08 SEE136 pKa = 4.03 IQSLLNKK143 pKa = 10.04 FSITKK148 pKa = 9.95 SGSKK152 pKa = 10.08 RR153 pKa = 11.84 FPTRR157 pKa = 11.84 AINGLEE163 pKa = 3.76 WHH165 pKa = 6.97 DD166 pKa = 3.68 TSEE169 pKa = 4.34 DD170 pKa = 3.42 QFKK173 pKa = 10.28 IHH175 pKa = 6.26 YY176 pKa = 8.83 KK177 pKa = 10.89 GNGEE181 pKa = 4.19 SKK183 pKa = 10.11 IAGSFKK189 pKa = 10.52 ISINVLTQNAKK200 pKa = 10.25
Molecular weight: 22.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.911
IPC_protein 10.833
Toseland 11.067
ProMoST 10.935
Dawson 11.125
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.33
Grimsley 11.155
Solomon 11.33
Lehninger 11.286
Nozaki 11.052
DTASelect 10.877
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.067
Patrickios 11.052
IPC_peptide 11.33
IPC2_peptide 9.911
IPC2.peptide.svr19 8.531
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1806
189
535
361.2
40.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.423 ± 0.797
1.218 ± 0.327
5.814 ± 0.883
6.257 ± 0.449
3.931 ± 0.644
5.592 ± 0.774
2.547 ± 0.28
5.482 ± 0.485
6.921 ± 1.036
7.198 ± 0.492
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.547 ± 0.619
4.817 ± 0.104
5.759 ± 0.841
3.599 ± 0.32
6.478 ± 0.788
10.299 ± 1.488
4.319 ± 0.619
6.368 ± 0.494
1.107 ± 0.173
3.322 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here