Methanolobus sp. SY-01

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanolobus; unclassified Methanolobus

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4E0PX16|A0A4E0PX16_9EURY Two-component system response regulator OS=Methanolobus sp. SY-01 OX=2052935 GN=CUN85_07250 PE=4 SV=1
MM1 pKa = 7.56ISKK4 pKa = 10.34KK5 pKa = 10.51SVILCFLSILLMASIDD21 pKa = 3.52ASIAQTIDD29 pKa = 2.86GTEE32 pKa = 3.66IQITSNRR39 pKa = 11.84SDD41 pKa = 3.1QYY43 pKa = 11.43FPAIYY48 pKa = 10.14DD49 pKa = 4.61DD50 pKa = 4.46IIVWTDD56 pKa = 2.84VRR58 pKa = 11.84YY59 pKa = 9.5EE60 pKa = 3.88SSDD63 pKa = 2.75IFLYY67 pKa = 11.16NMTSGEE73 pKa = 4.02EE74 pKa = 3.85LQITSNEE81 pKa = 4.07SEE83 pKa = 4.02EE84 pKa = 4.36RR85 pKa = 11.84VPDD88 pKa = 3.47IYY90 pKa = 11.38GDD92 pKa = 3.74IIVWEE97 pKa = 4.51DD98 pKa = 2.91WRR100 pKa = 11.84NGNWDD105 pKa = 3.25IYY107 pKa = 10.48IYY109 pKa = 11.0NLTSEE114 pKa = 4.7EE115 pKa = 3.9EE116 pKa = 4.16SALITNEE123 pKa = 3.2ADD125 pKa = 3.24QRR127 pKa = 11.84VPAIHH132 pKa = 7.37EE133 pKa = 4.07DD134 pKa = 3.77RR135 pKa = 11.84IVWEE139 pKa = 4.51DD140 pKa = 3.58LRR142 pKa = 11.84NGNWDD147 pKa = 2.71IYY149 pKa = 9.96MYY151 pKa = 10.26NISSGEE157 pKa = 3.87EE158 pKa = 3.65LQITDD163 pKa = 3.91NEE165 pKa = 4.36SDD167 pKa = 3.31QLYY170 pKa = 8.52PAVYY174 pKa = 9.92GDD176 pKa = 4.29LVTWMDD182 pKa = 4.1DD183 pKa = 3.8RR184 pKa = 11.84NGGWDD189 pKa = 3.07IFVYY193 pKa = 10.41DD194 pKa = 4.23LSSRR198 pKa = 11.84EE199 pKa = 3.99EE200 pKa = 3.99TQITANEE207 pKa = 4.36SIALYY212 pKa = 9.85PDD214 pKa = 3.48VYY216 pKa = 10.49EE217 pKa = 5.62DD218 pKa = 3.28RR219 pKa = 11.84VVWMDD224 pKa = 4.69DD225 pKa = 2.97RR226 pKa = 11.84NYY228 pKa = 10.59DD229 pKa = 3.86QNIFMYY235 pKa = 9.92NVSSEE240 pKa = 4.39EE241 pKa = 3.84EE242 pKa = 4.12LMVTTNEE249 pKa = 3.79SWQVVPKK256 pKa = 10.2IHH258 pKa = 7.42GDD260 pKa = 3.6RR261 pKa = 11.84IVWTDD266 pKa = 3.38EE267 pKa = 4.34RR268 pKa = 11.84NSNWDD273 pKa = 3.0ILGNSDD279 pKa = 3.17IYY281 pKa = 10.8MYY283 pKa = 10.07DD284 pKa = 3.0IPTGEE289 pKa = 4.29EE290 pKa = 3.8LQITTNEE297 pKa = 3.7EE298 pKa = 3.85RR299 pKa = 11.84QVYY302 pKa = 7.99PAIYY306 pKa = 9.31NDD308 pKa = 3.62TVVWEE313 pKa = 4.37DD314 pKa = 3.71RR315 pKa = 11.84RR316 pKa = 11.84NLNADD321 pKa = 3.46IYY323 pKa = 10.34IFTITGQNSTQQ334 pKa = 3.06

Molecular weight: 39.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4E0QRM6|A0A4E0QRM6_9EURY DUF11 domain-containing protein OS=Methanolobus sp. SY-01 OX=2052935 GN=CUN85_07365 PE=4 SV=1
MM1 pKa = 7.14SHH3 pKa = 5.37NTKK6 pKa = 9.76GQKK9 pKa = 9.82ARR11 pKa = 11.84LAKK14 pKa = 10.24AHH16 pKa = 6.28RR17 pKa = 11.84QNQRR21 pKa = 11.84VPVWAIVKK29 pKa = 7.42TNRR32 pKa = 11.84KK33 pKa = 8.88VVGHH37 pKa = 6.0PKK39 pKa = 8.65RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.99WRR44 pKa = 11.84RR45 pKa = 11.84SSLDD49 pKa = 3.23VKK51 pKa = 11.09

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2452

0

2452

719907

25

2895

293.6

32.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.922 ± 0.053

1.222 ± 0.023

6.151 ± 0.05

7.487 ± 0.054

3.976 ± 0.036

7.216 ± 0.044

1.946 ± 0.023

8.127 ± 0.046

6.049 ± 0.046

8.965 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.996 ± 0.027

4.278 ± 0.045

3.787 ± 0.03

2.572 ± 0.022

4.729 ± 0.039

6.77 ± 0.049

5.346 ± 0.043

7.236 ± 0.036

0.843 ± 0.017

3.383 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski