Capybara microvirus Cap3_SP_646
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W907|A0A4P8W907_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_646 OX=2585487 PE=4 SV=1
MM1 pKa = 6.52 FTIYY5 pKa = 10.43 HH6 pKa = 5.98 SLDD9 pKa = 3.14 FASARR14 pKa = 11.84 SRR16 pKa = 11.84 IEE18 pKa = 4.01 SNDD21 pKa = 3.31 PGDD24 pKa = 3.94 YY25 pKa = 10.51 LVHH28 pKa = 7.54 CEE30 pKa = 4.38 DD31 pKa = 4.73 FEE33 pKa = 5.32 NINDD37 pKa = 3.85 LVKK40 pKa = 10.76 RR41 pKa = 11.84 SLITRR46 pKa = 11.84 DD47 pKa = 3.47 SSCLFGRR54 pKa = 11.84 DD55 pKa = 3.66 PEE57 pKa = 4.5 CFYY60 pKa = 11.66 DD61 pKa = 5.76 DD62 pKa = 4.87 ALNDD66 pKa = 3.94 PKK68 pKa = 11.19 LQDD71 pKa = 4.28 DD72 pKa = 4.62 YY73 pKa = 11.35 EE74 pKa = 4.73 LSSKK78 pKa = 10.98 ASASKK83 pKa = 10.31 AAQQEE88 pKa = 4.33 AKK90 pKa = 10.09 ASSDD94 pKa = 3.26 AAVDD98 pKa = 4.51 LEE100 pKa = 4.54 ANLDD104 pKa = 3.78 NSVKK108 pKa = 10.58 QVDD111 pKa = 3.24
Molecular weight: 12.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.274
IPC2_protein 4.19
IPC_protein 4.151
Toseland 3.935
ProMoST 4.253
Dawson 4.139
Bjellqvist 4.329
Wikipedia 4.088
Rodwell 3.973
Grimsley 3.846
Solomon 4.139
Lehninger 4.101
Nozaki 4.253
DTASelect 4.52
Thurlkill 3.986
EMBOSS 4.088
Sillero 4.279
Patrickios 3.617
IPC_peptide 4.139
IPC2_peptide 4.253
IPC2.peptide.svr19 4.214
Protein with the highest isoelectric point:
>tr|A0A4P8W6I7|A0A4P8W6I7_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_646 OX=2585487 PE=4 SV=1
MM1 pKa = 7.78 ACRR4 pKa = 11.84 NYY6 pKa = 10.86 AIFHH10 pKa = 6.23 SDD12 pKa = 2.31 RR13 pKa = 11.84 GYY15 pKa = 10.83 FYY17 pKa = 10.88 APCGQCMEE25 pKa = 4.87 CRR27 pKa = 11.84 LKK29 pKa = 10.67 DD30 pKa = 3.51 LRR32 pKa = 11.84 DD33 pKa = 3.07 WYY35 pKa = 10.76 VRR37 pKa = 11.84 SRR39 pKa = 11.84 FEE41 pKa = 4.19 AKK43 pKa = 9.78 CKK45 pKa = 9.7 EE46 pKa = 4.17 RR47 pKa = 11.84 PFHH50 pKa = 6.63 YY51 pKa = 10.17 FLTLTYY57 pKa = 10.66 DD58 pKa = 3.86 DD59 pKa = 4.84 EE60 pKa = 4.49 NLPVDD65 pKa = 4.35 GFASRR70 pKa = 11.84 DD71 pKa = 3.63 DD72 pKa = 3.11 IKK74 pKa = 11.54 AFFKK78 pKa = 10.59 SLRR81 pKa = 11.84 HH82 pKa = 5.45 RR83 pKa = 11.84 FEE85 pKa = 4.37 GASIRR90 pKa = 11.84 YY91 pKa = 7.6 FATSDD96 pKa = 3.29 YY97 pKa = 10.94 GGKK100 pKa = 10.02 LGRR103 pKa = 11.84 AHH105 pKa = 6.31 FHH107 pKa = 6.78 AIVFSSVRR115 pKa = 11.84 IFANSIFNVWQKK127 pKa = 11.13 GFTKK131 pKa = 10.52 LKK133 pKa = 9.87 VLTPVSMRR141 pKa = 11.84 YY142 pKa = 8.35 CCKK145 pKa = 9.48 YY146 pKa = 9.11 TIKK149 pKa = 10.63 KK150 pKa = 9.26 RR151 pKa = 11.84 DD152 pKa = 3.54 LVGSEE157 pKa = 4.82 GYY159 pKa = 10.44 VNPRR163 pKa = 11.84 AFFRR167 pKa = 11.84 LVSKK171 pKa = 10.65 HH172 pKa = 5.38 YY173 pKa = 10.66 GEE175 pKa = 5.12 NGINYY180 pKa = 9.17 FNGTDD185 pKa = 3.34 NFVEE189 pKa = 4.05 FDD191 pKa = 3.49 GKK193 pKa = 10.95 KK194 pKa = 10.41 FGWPQYY200 pKa = 10.57 LIDD203 pKa = 5.57 KK204 pKa = 9.98 IVQNGLDD211 pKa = 3.45 PHH213 pKa = 7.04 LVFDD217 pKa = 4.48 KK218 pKa = 10.83 EE219 pKa = 3.92 ATKK222 pKa = 10.22 FYY224 pKa = 9.48 KK225 pKa = 9.96 TYY227 pKa = 10.75 RR228 pKa = 11.84 EE229 pKa = 3.74 ASYY232 pKa = 11.55 ACLSFVKK239 pKa = 10.41 QNNLRR244 pKa = 11.84 QLSGDD249 pKa = 3.59 DD250 pKa = 6.14 LYY252 pKa = 11.83 NEE254 pKa = 4.27 WNGGLRR260 pKa = 11.84 RR261 pKa = 11.84 AA262 pKa = 4.38
Molecular weight: 30.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.741
IPC2_protein 8.697
IPC_protein 8.609
Toseland 9.019
ProMoST 9.019
Dawson 9.414
Bjellqvist 9.326
Wikipedia 9.604
Rodwell 9.531
Grimsley 9.472
Solomon 9.458
Lehninger 9.414
Nozaki 9.385
DTASelect 9.209
Thurlkill 9.282
EMBOSS 9.531
Sillero 9.458
Patrickios 4.291
IPC_peptide 9.458
IPC2_peptide 8.185
IPC2.peptide.svr19 7.77
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1313
111
535
262.6
29.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.54 ± 1.607
1.447 ± 0.545
6.931 ± 1.237
4.417 ± 0.485
6.321 ± 0.909
7.007 ± 1.068
1.828 ± 0.531
4.646 ± 0.653
5.788 ± 1.197
8.225 ± 0.381
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.828 ± 0.313
6.55 ± 0.872
3.503 ± 0.927
3.58 ± 0.43
5.56 ± 0.742
8.759 ± 1.124
4.646 ± 0.613
5.712 ± 0.273
1.219 ± 0.275
4.494 ± 0.677
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here