Herbaspirillum sp. CF444

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Herbaspirillum; unclassified Herbaspirillum

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4973 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J3D9Q8|J3D9Q8_9BURK Arabinose efflux permease family protein OS=Herbaspirillum sp. CF444 OX=1144319 GN=PMI16_02878 PE=4 SV=1
MM1 pKa = 7.05ATAVSPKK8 pKa = 8.6PTPANQGNGANNAANTANAGQAITGSASVTMPDD41 pKa = 3.07QGGSFHH47 pKa = 7.03LNVPRR52 pKa = 11.84SAVKK56 pKa = 9.71HH57 pKa = 4.54VQVVDD62 pKa = 3.21VDD64 pKa = 3.88MVLQLGDD71 pKa = 3.23GSKK74 pKa = 10.22IVLAGGAMGAMDD86 pKa = 4.34EE87 pKa = 4.13KK88 pKa = 11.24SRR90 pKa = 11.84IIFADD95 pKa = 3.72ASQDD99 pKa = 3.29TGRR102 pKa = 11.84MLDD105 pKa = 3.76LVGKK109 pKa = 10.4IPLQNHH115 pKa = 5.58DD116 pKa = 3.51QSLILNSDD124 pKa = 3.76PNKK127 pKa = 10.75SADD130 pKa = 3.51RR131 pKa = 11.84TTNSDD136 pKa = 3.08NSGVPDD142 pKa = 3.64NHH144 pKa = 5.71FTSNNNSAQVNPAATSLLAKK164 pKa = 10.15IIQDD168 pKa = 3.48NAGSLTSNGLQNLALPSIPVPPAPPVTTGADD199 pKa = 3.46SLIKK203 pKa = 10.23QLSPEE208 pKa = 3.74IQEE211 pKa = 4.34RR212 pKa = 11.84PGSVLTNPVLGPQTPAMSLNLLNLTTQTQVGSVLFGSGGNAASASDD258 pKa = 3.8GSNAVQFSPQIINAGNDD275 pKa = 2.93VHH277 pKa = 6.82TIYY280 pKa = 10.15ATGSTTQSNFVKK292 pKa = 10.32IFEE295 pKa = 5.03LNVTGDD301 pKa = 3.81GSVKK305 pKa = 9.96TITITGVPSTMTILNAVNLGGGSYY329 pKa = 10.38QITPAAGEE337 pKa = 4.19KK338 pKa = 10.72SFDD341 pKa = 3.64LQVQYY346 pKa = 7.69TTHH349 pKa = 6.22VADD352 pKa = 4.07PTQPTAPTSTISFDD366 pKa = 3.72VAVLTTDD373 pKa = 3.59GLTHH377 pKa = 7.1LMDD380 pKa = 3.7SRR382 pKa = 11.84VVVIKK387 pKa = 10.48DD388 pKa = 3.06ASVYY392 pKa = 10.59TDD394 pKa = 3.67LTYY397 pKa = 10.99LDD399 pKa = 4.48PATGKK404 pKa = 10.01SVWVLPAQGVPHH416 pKa = 7.41EE417 pKa = 4.25IHH419 pKa = 6.9AGDD422 pKa = 4.06GGVTIYY428 pKa = 10.95GSNANDD434 pKa = 3.5SLYY437 pKa = 11.17GGAGVDD443 pKa = 3.86VIIGGSGNTYY453 pKa = 9.52FQGGAGADD461 pKa = 3.61TLTGGTQGVNTAGYY475 pKa = 7.4KK476 pKa = 9.52TSTAGVTVDD485 pKa = 3.91LTTGLGTGGDD495 pKa = 3.66AQGDD499 pKa = 4.03VLSNIQNLIGSAFNDD514 pKa = 3.41TFVANDD520 pKa = 3.37KK521 pKa = 10.93VNRR524 pKa = 11.84LDD526 pKa = 3.94GGSGGVDD533 pKa = 3.22TVSYY537 pKa = 10.27AASTSGVTVNLITGTGSGGYY557 pKa = 10.12AEE559 pKa = 5.24GDD561 pKa = 3.49TYY563 pKa = 11.71VAIQNATGSAYY574 pKa = 10.97NDD576 pKa = 2.9IFIANTLANRR586 pKa = 11.84FDD588 pKa = 4.1GGSGGSDD595 pKa = 3.0TVSYY599 pKa = 10.71ASSATGVTVNLSTGVGAGGSAAGDD623 pKa = 3.72TYY625 pKa = 11.46VHH627 pKa = 6.22IQNVIGSAFNDD638 pKa = 3.58TFVANIDD645 pKa = 3.73ANSFDD650 pKa = 4.24GGSGGLDD657 pKa = 2.99TVSYY661 pKa = 10.3ANSAAGVSVNFVSGRR676 pKa = 11.84GVGGDD681 pKa = 3.99AEE683 pKa = 4.71GDD685 pKa = 3.62TYY687 pKa = 11.87NRR689 pKa = 11.84IQNVIGSAYY698 pKa = 10.44DD699 pKa = 3.38DD700 pKa = 3.91VFIAGPDD707 pKa = 3.21SRR709 pKa = 11.84TFDD712 pKa = 3.77GGSGGSDD719 pKa = 3.07TVNYY723 pKa = 8.55GASSAGVTVNMVTMTGSGGYY743 pKa = 8.96AQNNTYY749 pKa = 11.03VNIQNATGSSYY760 pKa = 11.55SDD762 pKa = 3.25TFISSAVANHH772 pKa = 6.57FDD774 pKa = 3.64GGLAGSDD781 pKa = 3.43TVSYY785 pKa = 10.64ALSNAGVTVSLYY797 pKa = 10.84DD798 pKa = 3.69GSASGGYY805 pKa = 9.66AQGDD809 pKa = 4.01TLAHH813 pKa = 5.55IQNVIGSNFDD823 pKa = 3.29DD824 pKa = 3.8TFIASSEE831 pKa = 4.18VNNFEE836 pKa = 4.43GGGGVNTVSYY846 pKa = 9.31FYY848 pKa = 10.48ATGSVTIDD856 pKa = 3.21LTNTIGTGTGGSLYY870 pKa = 11.0ANGDD874 pKa = 3.76SFSNIQNLIGGASDD888 pKa = 3.61DD889 pKa = 3.96TFVASLAANKK899 pKa = 10.23LDD901 pKa = 4.06GGVSTATSHH910 pKa = 6.41NRR912 pKa = 11.84VSYY915 pKa = 9.95AASNAAVTVDD925 pKa = 3.69LNYY928 pKa = 10.94TNGTGTSGGYY938 pKa = 9.72AQGDD942 pKa = 3.65QLLNIQDD949 pKa = 3.85LTGSNFDD956 pKa = 3.66DD957 pKa = 3.79TFVASAANNNFDD969 pKa = 3.63GGAGSLHH976 pKa = 5.94NRR978 pKa = 11.84VSYY981 pKa = 10.4AASTSAVTVDD991 pKa = 3.58LTQTDD996 pKa = 3.95GSGTSGGFAQGDD1008 pKa = 3.96TLINIQDD1015 pKa = 3.92LTGSQYY1021 pKa = 11.38SDD1023 pKa = 3.18TFIASAAANAFNGGAGGSDD1042 pKa = 3.07TVSYY1046 pKa = 11.11ARR1048 pKa = 11.84ASDD1051 pKa = 3.95GVGVTVNLVTGAGSNGFAAGDD1072 pKa = 3.63TYY1074 pKa = 11.83LNIQNVIGSSYY1085 pKa = 11.21NDD1087 pKa = 3.11IFVASNDD1094 pKa = 3.3VNRR1097 pKa = 11.84FDD1099 pKa = 5.03GGGGSDD1105 pKa = 3.31TVSYY1109 pKa = 10.33ASSGAAVTVDD1119 pKa = 3.51LVGGTGAGGSAQGDD1133 pKa = 4.01TYY1135 pKa = 11.48INIQNVIGSAADD1147 pKa = 3.63DD1148 pKa = 4.17LFIASTAANAFEE1160 pKa = 5.09GGVSTAASHH1169 pKa = 6.45NRR1171 pKa = 11.84VSYY1174 pKa = 10.88VNSTVAEE1181 pKa = 4.26TVNLFTGAGTGGLGSFANGDD1201 pKa = 3.93TYY1203 pKa = 11.92SNIQDD1208 pKa = 3.51VTGGSGDD1215 pKa = 4.64DD1216 pKa = 3.06IFVANSAANNLDD1228 pKa = 3.6GGVSTATSHH1237 pKa = 6.47NRR1239 pKa = 11.84VSYY1242 pKa = 11.13ANSTVAEE1249 pKa = 4.3TVNLFTGNGSGGAGSLANGDD1269 pKa = 4.14TYY1271 pKa = 11.64TNIQDD1276 pKa = 3.94VTGGTADD1283 pKa = 4.01DD1284 pKa = 4.7LFIASAAANKK1294 pKa = 9.88FDD1296 pKa = 4.59GGASTATSHH1305 pKa = 6.46NRR1307 pKa = 11.84VSYY1310 pKa = 11.08ANSTVSEE1317 pKa = 4.32TVDD1320 pKa = 3.39LFLGTGSGGAGSLANGDD1337 pKa = 4.14TYY1339 pKa = 11.64TNIQDD1344 pKa = 3.94VTGGTADD1351 pKa = 5.03DD1352 pKa = 3.64IFVANTAANAFDD1364 pKa = 4.35GGTGSLHH1371 pKa = 6.27NRR1373 pKa = 11.84VSYY1376 pKa = 11.11ANSSAAEE1383 pKa = 4.12TVDD1386 pKa = 3.78LFLGAGSGTFAAGDD1400 pKa = 3.83TYY1402 pKa = 11.98ANIQDD1407 pKa = 3.77VTGGSGDD1414 pKa = 3.7DD1415 pKa = 3.46TFVASLAANSFDD1427 pKa = 4.36GGTSTAASHH1436 pKa = 6.37NRR1438 pKa = 11.84VSYY1441 pKa = 11.17ANSTASEE1448 pKa = 4.3TVNLFTGVGTGTLAQGDD1465 pKa = 4.63TYY1467 pKa = 11.93VNIQDD1472 pKa = 4.0VTGGTADD1479 pKa = 4.01DD1480 pKa = 4.7LFIASAAANKK1490 pKa = 9.89FDD1492 pKa = 3.9GGTGSLHH1499 pKa = 6.21NRR1501 pKa = 11.84VSYY1504 pKa = 10.44TNSTASEE1511 pKa = 4.26TVDD1514 pKa = 3.76LFLGTGSGTLAQGDD1528 pKa = 4.52TYY1530 pKa = 11.93VNIQDD1535 pKa = 4.0VTGGTADD1542 pKa = 3.47DD1543 pKa = 4.02TFIASAAANNFDD1555 pKa = 3.84GGTGSLHH1562 pKa = 6.27NRR1564 pKa = 11.84VSYY1567 pKa = 11.34ANSTVSEE1574 pKa = 4.37TVNLLTGAGTGGTNSLANGDD1594 pKa = 4.32TYY1596 pKa = 11.64TNIQDD1601 pKa = 3.94VTGGTADD1608 pKa = 4.01DD1609 pKa = 4.7LFIASAAANKK1619 pKa = 9.9FDD1621 pKa = 4.61GGTSTATSHH1630 pKa = 6.27NRR1632 pKa = 11.84VSYY1635 pKa = 8.69ATSTVSEE1642 pKa = 4.37TVNLFTGVGTGGVGSLANGDD1662 pKa = 3.95TYY1664 pKa = 11.97VNIQDD1669 pKa = 4.0VTGGTADD1676 pKa = 4.65DD1677 pKa = 4.17IFVASAAANSFDD1689 pKa = 3.58GGTGSLHH1696 pKa = 6.34NLVSYY1701 pKa = 9.46TNSTASEE1708 pKa = 4.26TVDD1711 pKa = 3.76LFLGTGSGTLAQGDD1725 pKa = 4.81TYY1727 pKa = 11.56TNIQDD1732 pKa = 3.94VTGGTADD1739 pKa = 3.51DD1740 pKa = 3.9TFIASLAANKK1750 pKa = 9.9FDD1752 pKa = 3.89GGTGSLHH1759 pKa = 6.27NRR1761 pKa = 11.84VSYY1764 pKa = 11.33ANSTASEE1771 pKa = 4.3TVNLFTGAGTGTLAQGDD1788 pKa = 4.9TYY1790 pKa = 11.48TNIQDD1795 pKa = 3.67VPAVRR1800 pKa = 11.84PTICSSPAQRR1810 pKa = 11.84PINSMAAPARR1820 pKa = 11.84RR1821 pKa = 11.84LRR1823 pKa = 11.84TTVV1826 pKa = 2.67

Molecular weight: 182.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J3D3V4|J3D3V4_9BURK 30S ribosomal protein S6 OS=Herbaspirillum sp. CF444 OX=1144319 GN=rpsF PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.8RR41 pKa = 11.84LAAVV45 pKa = 3.52

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4973

0

4973

1635139

22

4843

328.8

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.628 ± 0.043

0.85 ± 0.011

5.335 ± 0.024

5.18 ± 0.033

3.758 ± 0.023

7.851 ± 0.037

2.17 ± 0.021

5.494 ± 0.026

4.076 ± 0.035

10.472 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.017

3.299 ± 0.029

4.648 ± 0.024

4.114 ± 0.026

5.985 ± 0.029

6.01 ± 0.037

5.344 ± 0.036

7.346 ± 0.029

1.259 ± 0.015

2.544 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski