Enterococcus italicus (strain DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus italicus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6LH91|E6LH91_ENTI1 Spermidine/putrescine import ATP-binding protein PotA OS=Enterococcus italicus (strain DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5) OX=888064 GN=potA PE=3 SV=1
MM1 pKa = 7.48TEE3 pKa = 3.86HH4 pKa = 5.97THH6 pKa = 6.67DD7 pKa = 4.5HH8 pKa = 6.07DD9 pKa = 5.13HH10 pKa = 7.06EE11 pKa = 4.45GHH13 pKa = 5.2EE14 pKa = 5.0HH15 pKa = 5.56ITLVDD20 pKa = 3.67DD21 pKa = 4.66EE22 pKa = 5.09GNEE25 pKa = 3.94TLYY28 pKa = 10.99EE29 pKa = 4.03ILLTIDD35 pKa = 3.3GQEE38 pKa = 3.92EE39 pKa = 4.06FGRR42 pKa = 11.84NYY44 pKa = 10.54VLLYY48 pKa = 9.2PAGVPEE54 pKa = 4.36EE55 pKa = 4.23EE56 pKa = 4.46DD57 pKa = 3.82VEE59 pKa = 4.4LLAYY63 pKa = 10.17AYY65 pKa = 10.29VEE67 pKa = 4.7KK68 pKa = 10.43EE69 pKa = 4.01DD70 pKa = 4.22GMEE73 pKa = 4.7GDD75 pKa = 4.04LQQIEE80 pKa = 4.62TDD82 pKa = 3.87QEE84 pKa = 3.31WDD86 pKa = 3.39MIEE89 pKa = 4.3EE90 pKa = 4.21VFNTFMAEE98 pKa = 4.02EE99 pKa = 3.96EE100 pKa = 4.43AEE102 pKa = 4.09

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6LEM6|E6LEM6_ENTI1 Uncharacterized protein OS=Enterococcus italicus (strain DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5) OX=888064 GN=HMPREF9088_0816 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 9.14RR10 pKa = 11.84KK11 pKa = 8.54HH12 pKa = 5.23QKK14 pKa = 8.76VHH16 pKa = 5.58GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.33GRR39 pKa = 11.84KK40 pKa = 9.06VISAA44 pKa = 4.05

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2404

0

2404

685835

39

1571

285.3

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.51 ± 0.055

0.658 ± 0.014

5.19 ± 0.044

6.952 ± 0.063

4.592 ± 0.045

6.429 ± 0.053

1.97 ± 0.022

7.372 ± 0.048

6.741 ± 0.05

9.913 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.025

4.294 ± 0.037

3.396 ± 0.029

4.619 ± 0.046

4.011 ± 0.041

6.015 ± 0.042

6.026 ± 0.042

7.049 ± 0.042

0.932 ± 0.017

3.66 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski