Desulfuribacillus alkaliarsenatis
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2879 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E5G3B0|A0A1E5G3B0_9BACL Uncharacterized protein OS=Desulfuribacillus alkaliarsenatis OX=766136 GN=BHF68_05585 PE=4 SV=1
MM1 pKa = 7.98 RR2 pKa = 11.84 FTKK5 pKa = 10.18 KK6 pKa = 10.13 VVSICVIFMFILGTALPTGLAAEE29 pKa = 4.13 QQIQINFNGQSYY41 pKa = 10.09 NADD44 pKa = 3.92 FYY46 pKa = 11.78 LEE48 pKa = 5.16 DD49 pKa = 6.1 DD50 pKa = 3.14 ITYY53 pKa = 10.56 INVDD57 pKa = 3.85 SLKK60 pKa = 10.36 KK61 pKa = 10.39 IPGLEE66 pKa = 4.03 VNEE69 pKa = 4.1 YY70 pKa = 10.79 NYY72 pKa = 10.68 VPLRR76 pKa = 11.84 AFFEE80 pKa = 4.69 GQGATVTWNAQSKK93 pKa = 10.61 VIDD96 pKa = 3.71 ISWRR100 pKa = 11.84 EE101 pKa = 4.05 TNDD104 pKa = 2.24 GWTANDD110 pKa = 3.58 LVVKK114 pKa = 10.69 AEE116 pKa = 4.21 EE117 pKa = 4.0 VLKK120 pKa = 10.66 EE121 pKa = 3.85 YY122 pKa = 9.39 NTYY125 pKa = 10.9 RR126 pKa = 11.84 MAGDD130 pKa = 3.49 GTIDD134 pKa = 3.54 ISISGNDD141 pKa = 3.49 AADD144 pKa = 3.78 AFPFDD149 pKa = 4.0 GMTLAIEE156 pKa = 4.88 GSFSQEE162 pKa = 3.42 PLAMYY167 pKa = 7.52 MKK169 pKa = 9.48 QTMTMPINFEE179 pKa = 4.05 EE180 pKa = 4.19 MGLTEE185 pKa = 4.95 EE186 pKa = 4.58 EE187 pKa = 4.13 LALMGMDD194 pKa = 3.1 TSMVTEE200 pKa = 4.5 MVWKK204 pKa = 10.65 DD205 pKa = 3.1 NAIYY209 pKa = 10.46 QRR211 pKa = 11.84 ILPLQEE217 pKa = 3.3 QWIVNNIEE225 pKa = 4.22 DD226 pKa = 4.69 LGMSDD231 pKa = 3.85 TFNDD235 pKa = 4.28 LLQMTPQQNLEE246 pKa = 4.11 LMTKK250 pKa = 10.16 YY251 pKa = 10.74 GVFYY255 pKa = 10.52 TFGDD259 pKa = 3.85 DD260 pKa = 3.91 VEE262 pKa = 4.54 VDD264 pKa = 3.82 GIEE267 pKa = 4.33 YY268 pKa = 10.62 YY269 pKa = 10.7 VVNSTLSADD278 pKa = 3.67 TFRR281 pKa = 11.84 NLMEE285 pKa = 5.99 DD286 pKa = 3.25 MFDD289 pKa = 4.51 DD290 pKa = 4.96 LMPPEE295 pKa = 5.0 LLTEE299 pKa = 4.29 EE300 pKa = 4.15 SDD302 pKa = 3.8 EE303 pKa = 4.31 VSVMIDD309 pKa = 3.32 EE310 pKa = 4.53 IFANINMNFYY320 pKa = 10.54 QQTLINKK327 pKa = 7.44 EE328 pKa = 4.12 TLTTDD333 pKa = 3.68 FLIYY337 pKa = 10.94 DD338 pKa = 3.91 MVMSLALADD347 pKa = 3.98 MFSQEE352 pKa = 5.89 DD353 pKa = 4.33 GLQEE357 pKa = 4.16 DD358 pKa = 4.87 APEE361 pKa = 4.24 SMLMSMSGVYY371 pKa = 9.94 HH372 pKa = 7.17 LYY374 pKa = 10.89 DD375 pKa = 3.7 FGVEE379 pKa = 3.9 IEE381 pKa = 4.82 LPDD384 pKa = 3.48 VSDD387 pKa = 5.62 AITQEE392 pKa = 4.36 EE393 pKa = 4.92 YY394 pKa = 10.2 MEE396 pKa = 3.92 QQMQLFEE403 pKa = 4.34 SLEE406 pKa = 4.21 PEE408 pKa = 4.41 VQQ410 pKa = 3.1
Molecular weight: 46.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.63
IPC_protein 3.63
Toseland 3.427
ProMoST 3.77
Dawson 3.605
Bjellqvist 3.757
Wikipedia 3.516
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.554
Nozaki 3.719
DTASelect 3.91
Thurlkill 3.465
EMBOSS 3.528
Sillero 3.745
Patrickios 1.24
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A1E5G318|A0A1E5G318_9BACL Arsenic-transporting ATPase OS=Desulfuribacillus alkaliarsenatis OX=766136 GN=BHF68_04430 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPNTRR10 pKa = 11.84 KK11 pKa = 9.78 RR12 pKa = 11.84 KK13 pKa = 8.71 KK14 pKa = 8.74 VHH16 pKa = 5.61 GFRR19 pKa = 11.84 KK20 pKa = 9.89 RR21 pKa = 11.84 MSSSSGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.38 KK37 pKa = 9.69 GRR39 pKa = 11.84 KK40 pKa = 8.71 VLSAA44 pKa = 4.05
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2879
0
2879
915468
37
2151
318.0
35.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.119 ± 0.047
0.867 ± 0.019
5.354 ± 0.036
7.215 ± 0.049
4.078 ± 0.033
6.485 ± 0.042
1.94 ± 0.021
8.811 ± 0.047
6.574 ± 0.052
9.604 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.636 ± 0.022
4.895 ± 0.038
3.373 ± 0.022
3.985 ± 0.035
4.213 ± 0.032
5.807 ± 0.029
5.366 ± 0.028
7.074 ± 0.036
0.886 ± 0.015
3.719 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here