Lactobacillus phage LpeD

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Elpedvirus; Lactobacillus virus LpeD

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291I9K8|A0A291I9K8_9CAUD Transmembrane fragile-X-F protein OS=Lactobacillus phage LpeD OX=2041210 GN=LpeD_118 PE=4 SV=1
MM1 pKa = 7.86GYY3 pKa = 8.78TDD5 pKa = 4.98GVAQRR10 pKa = 11.84MSEE13 pKa = 4.06SSLKK17 pKa = 9.64GTLFQLDD24 pKa = 3.68KK25 pKa = 10.58FTYY28 pKa = 10.19QDD30 pKa = 3.99DD31 pKa = 4.21NFNEE35 pKa = 3.99NDD37 pKa = 4.25LDD39 pKa = 4.13TEE41 pKa = 4.35SLSDD45 pKa = 4.13FLDD48 pKa = 4.06DD49 pKa = 6.66KK50 pKa = 11.39IDD52 pKa = 4.58LYY54 pKa = 11.38QSDD57 pKa = 4.54YY58 pKa = 11.91ADD60 pKa = 4.29LLRR63 pKa = 11.84DD64 pKa = 4.62GYY66 pKa = 11.43CYY68 pKa = 10.48DD69 pKa = 3.72DD70 pKa = 4.67SEE72 pKa = 4.85NKK74 pKa = 10.12VILTIEE80 pKa = 3.98NDD82 pKa = 3.37GFTVEE87 pKa = 4.61IIEE90 pKa = 6.13DD91 pKa = 3.65IYY93 pKa = 11.89NWIDD97 pKa = 3.38EE98 pKa = 4.17NSQGGLYY105 pKa = 10.44YY106 pKa = 10.11ILDD109 pKa = 3.87EE110 pKa = 5.53DD111 pKa = 4.39KK112 pKa = 11.42DD113 pKa = 4.09LVGARR118 pKa = 11.84LGVCGEE124 pKa = 4.78HH125 pKa = 6.72IGQVSVWLDD134 pKa = 3.36TVKK137 pKa = 10.78GVFEE141 pKa = 4.18QAGTTYY147 pKa = 10.61SISDD151 pKa = 3.69EE152 pKa = 4.1NKK154 pKa = 9.72EE155 pKa = 4.37AINDD159 pKa = 3.92ALEE162 pKa = 4.14EE163 pKa = 4.04MLDD166 pKa = 3.84CC167 pKa = 6.34

Molecular weight: 18.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291I9L2|A0A291I9L2_9CAUD Uncharacterized protein OS=Lactobacillus phage LpeD OX=2041210 GN=LpeD_1 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 9.64NDD4 pKa = 4.08KK5 pKa = 10.15IIKK8 pKa = 9.6VVLALVAGLTLSLVAFKK25 pKa = 10.33PSSVDD30 pKa = 2.81ASQRR34 pKa = 11.84PNYY37 pKa = 10.45AYY39 pKa = 9.59TLTRR43 pKa = 11.84TKK45 pKa = 10.03QHH47 pKa = 5.7AVKK50 pKa = 10.38LVNTGKK56 pKa = 8.59TEE58 pKa = 3.43NMYY61 pKa = 10.57RR62 pKa = 11.84VTIKK66 pKa = 10.46QRR68 pKa = 11.84DD69 pKa = 3.56HH70 pKa = 6.61SKK72 pKa = 9.53WYY74 pKa = 10.9YY75 pKa = 7.88MALNARR81 pKa = 11.84QSWVVNQSGKK91 pKa = 10.25YY92 pKa = 8.13SVTVRR97 pKa = 11.84RR98 pKa = 11.84VSKK101 pKa = 10.81SDD103 pKa = 3.2EE104 pKa = 4.01KK105 pKa = 11.29RR106 pKa = 11.84NADD109 pKa = 3.57PRR111 pKa = 11.84NHH113 pKa = 5.94FTPQGISNTQRR124 pKa = 11.84TIWNRR129 pKa = 3.1

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

30666

44

1420

306.7

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.472 ± 0.26

0.564 ± 0.068

7.07 ± 0.221

5.472 ± 0.237

3.864 ± 0.137

6.421 ± 0.317

1.471 ± 0.099

6.77 ± 0.205

7.859 ± 0.339

8.302 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.071

7.311 ± 0.153

2.99 ± 0.163

3.639 ± 0.155

3.342 ± 0.126

8.449 ± 0.31

6.45 ± 0.337

6.222 ± 0.145

1.07 ± 0.096

5.129 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski