Curtobacterium sp. MCBD17_040

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Curtobacterium; unclassified Curtobacterium

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W1ZRA1|A0A2W1ZRA1_9MICO Uncharacterized protein OS=Curtobacterium sp. MCBD17_040 OX=2175674 GN=DEI94_15800 PE=4 SV=1
MM1 pKa = 7.81AGTFSGIQSAYY12 pKa = 10.55SGLTAAQTALNVVGQNIDD30 pKa = 3.41NASTAGYY37 pKa = 8.94VRR39 pKa = 11.84QRR41 pKa = 11.84VEE43 pKa = 3.77QTAVGSPAQVGLAGGTTQAGQGVLVTAISNLSSVFLDD80 pKa = 3.54SQVRR84 pKa = 11.84AASAANSYY92 pKa = 9.36TGARR96 pKa = 11.84ATGYY100 pKa = 9.39TSVEE104 pKa = 4.16TALGEE109 pKa = 4.26PSDD112 pKa = 4.78DD113 pKa = 3.75ALSSQMNSFWSAWSDD128 pKa = 3.67VSNQPGNDD136 pKa = 3.49APVGALLGQAQTLTTQIGDD155 pKa = 4.16AYY157 pKa = 10.98RR158 pKa = 11.84SMQIGWSNTYY168 pKa = 10.1QSAGTEE174 pKa = 3.91VDD176 pKa = 3.24QLNALTSQVATLNGQIRR193 pKa = 11.84STIASGNNANEE204 pKa = 4.16LTDD207 pKa = 3.82EE208 pKa = 4.52RR209 pKa = 11.84SQLAAQISALAGGSVAQNSDD229 pKa = 2.78NTINITIGGSNVVDD243 pKa = 3.64GTNARR248 pKa = 11.84QVSLSGADD256 pKa = 3.41TLEE259 pKa = 4.26NASANPVTITWSDD272 pKa = 3.61QVGGAPLSLTGGSIAGNLSLLAGANPAGTGGAYY305 pKa = 9.9AQAADD310 pKa = 4.36ALNTLATSIATSVNAVSEE328 pKa = 4.43TGATANGTTGTDD340 pKa = 4.0FFSLTAGQPAALSISVIPTNADD362 pKa = 2.78GVAVGTPGAGANDD375 pKa = 3.92GSVADD380 pKa = 4.5QISQIGTGATSPSNQWSTYY399 pKa = 7.79VVSLGTQSSVEE410 pKa = 4.31TQQSTLATTTYY421 pKa = 10.49NSAVTSQQSQEE432 pKa = 4.26GVDD435 pKa = 3.85LDD437 pKa = 4.12EE438 pKa = 5.32EE439 pKa = 5.01SMNLLEE445 pKa = 5.13AQHH448 pKa = 6.98AYY450 pKa = 10.13QGAARR455 pKa = 11.84VMTAMDD461 pKa = 3.44QMLDD465 pKa = 3.21QLINHH470 pKa = 7.32TGTVGISS477 pKa = 2.98

Molecular weight: 47.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W1YIC5|A0A2W1YIC5_9MICO Uncharacterized protein OS=Curtobacterium sp. MCBD17_040 OX=2175674 GN=DEI94_07445 PE=4 SV=1
MM1 pKa = 7.8PRR3 pKa = 11.84RR4 pKa = 11.84CSTARR9 pKa = 11.84GGPGTPPTRR18 pKa = 11.84RR19 pKa = 11.84TPTARR24 pKa = 11.84TPRR27 pKa = 11.84RR28 pKa = 11.84PRR30 pKa = 11.84RR31 pKa = 11.84HH32 pKa = 5.61RR33 pKa = 11.84AAPTRR38 pKa = 11.84VVATNVPRR46 pKa = 11.84RR47 pKa = 11.84APPPPGRR54 pKa = 11.84RR55 pKa = 11.84ARR57 pKa = 11.84AARR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84AA63 pKa = 3.21

Molecular weight: 7.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3754

0

3754

1159833

19

1947

309.0

32.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.702 ± 0.061

0.489 ± 0.009

6.534 ± 0.037

4.966 ± 0.042

2.933 ± 0.025

9.008 ± 0.037

2.206 ± 0.022

3.785 ± 0.029

1.745 ± 0.031

9.374 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.694 ± 0.015

1.876 ± 0.025

5.568 ± 0.034

2.881 ± 0.027

7.775 ± 0.053

5.542 ± 0.042

6.779 ± 0.05

9.717 ± 0.047

1.506 ± 0.018

1.921 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski