Staphylococcus phage Henu2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus Henu2

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410T4A9|A0A410T4A9_9CAUD Tail protein OS=Staphylococcus phage Henu2 OX=2492948 GN=Henu2_gp62 PE=4 SV=1
MM1 pKa = 7.67IKK3 pKa = 10.3QILRR7 pKa = 11.84LLFLLAMYY15 pKa = 10.04EE16 pKa = 3.83LGKK19 pKa = 10.83YY20 pKa = 7.27VTEE23 pKa = 3.84QVYY26 pKa = 10.94IMMTANDD33 pKa = 4.14DD34 pKa = 3.74VEE36 pKa = 4.99APSDD40 pKa = 3.68FAKK43 pKa = 10.93LSDD46 pKa = 3.64QSDD49 pKa = 3.47LMRR52 pKa = 11.84AEE54 pKa = 4.08VSEE57 pKa = 4.21

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410T4X0|A0A410T4X0_9CAUD Uncharacterized protein OS=Staphylococcus phage Henu2 OX=2492948 GN=Henu2_gp59 PE=4 SV=1
MM1 pKa = 6.63TSRR4 pKa = 11.84YY5 pKa = 8.73IGYY8 pKa = 8.71MSNDD12 pKa = 3.71EE13 pKa = 4.57LMSMLPAEE21 pKa = 4.27WNDD24 pKa = 3.65WIIGARR30 pKa = 11.84QALIDD35 pKa = 3.58QRR37 pKa = 11.84DD38 pKa = 3.38IALYY42 pKa = 8.51GAQYY46 pKa = 10.82NAVAQAGKK54 pKa = 9.21SLKK57 pKa = 10.52RR58 pKa = 11.84FVRR61 pKa = 11.84QNEE64 pKa = 3.97RR65 pKa = 11.84EE66 pKa = 4.01HH67 pKa = 7.72YY68 pKa = 9.88IIRR71 pKa = 11.84GQEE74 pKa = 3.73GEE76 pKa = 4.3YY77 pKa = 10.96EE78 pKa = 4.0RR79 pKa = 11.84MKK81 pKa = 10.72QRR83 pKa = 11.84EE84 pKa = 3.83LAKK87 pKa = 10.37NKK89 pKa = 9.95RR90 pKa = 11.84KK91 pKa = 10.09RR92 pKa = 11.84EE93 pKa = 3.88IQKK96 pKa = 9.92QGTRR100 pKa = 11.84KK101 pKa = 9.39FLNSLKK107 pKa = 9.64TSHH110 pKa = 7.02KK111 pKa = 10.74GGG113 pKa = 3.51

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13444

36

989

216.8

24.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.78 ± 0.38

0.424 ± 0.074

6.159 ± 0.29

7.721 ± 0.528

4.195 ± 0.221

5.705 ± 0.385

1.703 ± 0.126

7.647 ± 0.305

9.142 ± 0.415

7.55 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.16

6.65 ± 0.291

2.67 ± 0.221

4.508 ± 0.218

4.121 ± 0.234

6.084 ± 0.262

6.382 ± 0.238

5.713 ± 0.317

1.175 ± 0.156

4.217 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski