Nanohaloarchaea archaeon

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Nanohaloarchaea; unclassified Nanohaloarchaea

Average proteome isoelectric point is 5.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1074 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N0NRN8|A0A6N0NRN8_9EURY Glycosyltransferase OS=Nanohaloarchaea archaeon OX=2594798 GN=GKQ38_03465 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84KK3 pKa = 9.38YY4 pKa = 10.56LALLGTLVFLLLSIPAATASTVTWSDD30 pKa = 3.08SSDD33 pKa = 3.03WNNAVSDD40 pKa = 3.82FGVVHH45 pKa = 6.63EE46 pKa = 5.05SVTNTDD52 pKa = 3.58HH53 pKa = 7.42NDD55 pKa = 3.11AGKK58 pKa = 9.4LQKK61 pKa = 10.41GYY63 pKa = 9.5PIQNPVVSYY72 pKa = 10.94GLVGYY77 pKa = 9.07WPLQEE82 pKa = 5.62DD83 pKa = 4.22SGDD86 pKa = 3.48TAYY89 pKa = 10.81DD90 pKa = 3.55FSGYY94 pKa = 10.58NDD96 pKa = 3.89ASASAPTQGSSGILGSSSYY115 pKa = 11.26YY116 pKa = 10.74FNTEE120 pKa = 3.57SADD123 pKa = 3.17ASTIDD128 pKa = 3.78LEE130 pKa = 4.64YY131 pKa = 11.06YY132 pKa = 10.72SFNAWINDD140 pKa = 3.63EE141 pKa = 3.81GSTYY145 pKa = 9.61YY146 pKa = 10.82ARR148 pKa = 11.84AMSNGRR154 pKa = 11.84DD155 pKa = 3.7GTDD158 pKa = 3.27PSATYY163 pKa = 11.59AMGSRR168 pKa = 11.84NGWPTAVMRR177 pKa = 11.84KK178 pKa = 9.5NGNTYY183 pKa = 10.09TIGSGLNPSDD193 pKa = 2.86WTMVTVTYY201 pKa = 10.83DD202 pKa = 3.2GDD204 pKa = 3.89TLKK207 pKa = 11.07VYY209 pKa = 10.5TDD211 pKa = 3.78GSLTGSSTAPSGPIGDD227 pKa = 3.52VTGPFNLGSTEE238 pKa = 4.09RR239 pKa = 11.84YY240 pKa = 8.47PGNEE244 pKa = 4.22GITGYY249 pKa = 10.29LQEE252 pKa = 4.28ASLYY256 pKa = 10.24NRR258 pKa = 11.84ALSASEE264 pKa = 3.78IQSLYY269 pKa = 10.32EE270 pKa = 3.82VAATPGTLTTGWKK283 pKa = 8.37TFSSNRR289 pKa = 11.84DD290 pKa = 3.4MNSLQLEE297 pKa = 4.35NVQASLNGGSITVYY311 pKa = 10.81VEE313 pKa = 4.06TQNGEE318 pKa = 3.89VSDD321 pKa = 5.18PISLDD326 pKa = 3.39GSGGPYY332 pKa = 10.15SVTGLSTEE340 pKa = 4.0EE341 pKa = 3.74DD342 pKa = 3.46QFRR345 pKa = 11.84LDD347 pKa = 3.41IRR349 pKa = 11.84LDD351 pKa = 3.64NSDD354 pKa = 3.56VTSTPTLSAIDD365 pKa = 3.66LTGNKK370 pKa = 9.64ANSPPAAPSNEE381 pKa = 4.0DD382 pKa = 3.47PADD385 pKa = 3.72NADD388 pKa = 4.09PVSLSPSLSVFVDD401 pKa = 3.98DD402 pKa = 5.69PDD404 pKa = 5.59GDD406 pKa = 4.25SMDD409 pKa = 3.21VTFYY413 pKa = 11.67VDD415 pKa = 3.36TNNDD419 pKa = 2.78GSYY422 pKa = 8.26EE423 pKa = 4.21TTKK426 pKa = 10.71TNTGVASGSTTSVSLSSLATSQTVNWYY453 pKa = 9.64VVADD457 pKa = 4.44DD458 pKa = 5.33GRR460 pKa = 11.84ATTQSSTWSFTTVGVPSITSAPSPSDD486 pKa = 3.17GAVDD490 pKa = 3.57VSTSKK495 pKa = 11.03NLAIDD500 pKa = 3.52VSHH503 pKa = 7.89PDD505 pKa = 3.48NLDD508 pKa = 3.26MTVEE512 pKa = 4.44FSMDD516 pKa = 2.94GSYY519 pKa = 10.86VGSDD523 pKa = 3.21TVAGSGTASYY533 pKa = 10.66SPSLGYY539 pKa = 10.11SEE541 pKa = 4.59DD542 pKa = 3.96HH543 pKa = 6.29SWSVDD548 pKa = 3.19VYY550 pKa = 8.26TQGYY554 pKa = 6.77STQTSGGAWNFHH566 pKa = 5.13TVHH569 pKa = 6.84DD570 pKa = 4.74VSASLSGPSKK580 pKa = 10.76SPASIDD586 pKa = 3.44PTLSATVSHH595 pKa = 6.86SDD597 pKa = 3.04SAEE600 pKa = 3.94TVTVDD605 pKa = 5.55FVNTDD610 pKa = 3.12NGNTICSVSGIGNAEE625 pKa = 4.11TATCDD630 pKa = 3.23TSGSSFGSSPGTTYY644 pKa = 10.72NWEE647 pKa = 4.09VQLSGDD653 pKa = 4.14EE654 pKa = 4.78GSEE657 pKa = 4.06SWTSSQRR664 pKa = 11.84SFTTISNPNVNRR676 pKa = 11.84VEE678 pKa = 4.28PGSNSGVSTDD688 pKa = 3.3QNLVVDD694 pKa = 4.61VSHH697 pKa = 8.05PDD699 pKa = 3.3GKK701 pKa = 10.78NMNVEE706 pKa = 3.84FMIRR710 pKa = 11.84KK711 pKa = 9.4AGQTSFSTVDD721 pKa = 2.98TKK723 pKa = 11.16TDD725 pKa = 2.99VSGQVSANPSLSDD738 pKa = 3.15GTSYY742 pKa = 10.53EE743 pKa = 3.55WRR745 pKa = 11.84VDD747 pKa = 3.28VRR749 pKa = 11.84TVGYY753 pKa = 8.28STDD756 pKa = 3.33TMSSIWGFTTNYY768 pKa = 8.53VPSVSGITKK777 pKa = 9.46YY778 pKa = 10.56DD779 pKa = 3.27ASDD782 pKa = 2.87GHH784 pKa = 6.71AFRR787 pKa = 11.84NVSAIISDD795 pKa = 3.54QDD797 pKa = 3.49GDD799 pKa = 4.04DD800 pKa = 4.3TIVDD804 pKa = 3.73ANLTVDD810 pKa = 3.4NDD812 pKa = 4.1GGLEE816 pKa = 4.1TYY818 pKa = 10.49VEE820 pKa = 4.17YY821 pKa = 11.22DD822 pKa = 3.18EE823 pKa = 5.64VSIDD827 pKa = 3.31RR828 pKa = 11.84SYY830 pKa = 11.97GGDD833 pKa = 3.42DD834 pKa = 3.3NQANVTFGRR843 pKa = 11.84VNVGDD848 pKa = 4.44SNWGLTGIDD857 pKa = 3.94LQVEE861 pKa = 4.96GIDD864 pKa = 3.48QEE866 pKa = 4.93GNVGSNTLSNVQFPNHH882 pKa = 6.68APTVASGYY890 pKa = 9.91SYY892 pKa = 11.31SDD894 pKa = 3.24SPQAHH899 pKa = 7.15SYY901 pKa = 8.86TLSIDD906 pKa = 3.62AQDD909 pKa = 3.74VDD911 pKa = 4.32DD912 pKa = 4.55TDD914 pKa = 4.63EE915 pKa = 5.1EE916 pKa = 4.52IASCTVDD923 pKa = 4.69HH924 pKa = 7.26NDD926 pKa = 3.77GDD928 pKa = 4.49GNSYY932 pKa = 8.98TGKK935 pKa = 10.4AGSLASGEE943 pKa = 4.26TATCSFTIDD952 pKa = 3.66NSTSGYY958 pKa = 10.5RR959 pKa = 11.84FGEE962 pKa = 4.45MINHH966 pKa = 6.5RR967 pKa = 11.84VTFKK971 pKa = 10.55DD972 pKa = 3.22AHH974 pKa = 6.12GKK976 pKa = 7.58TVTSTWASHH985 pKa = 7.23RR986 pKa = 11.84IPNSLPSATNLRR998 pKa = 11.84PASDD1002 pKa = 3.61NTSYY1006 pKa = 11.44DD1007 pKa = 3.56ADD1009 pKa = 4.03LKK1011 pKa = 10.42ATYY1014 pKa = 9.99NDD1016 pKa = 3.81PDD1018 pKa = 4.77GDD1020 pKa = 3.95VGNLTFYY1027 pKa = 9.88NTTSGEE1033 pKa = 4.07EE1034 pKa = 3.92LGAVADD1040 pKa = 4.73LAPGEE1045 pKa = 4.3QGTVDD1050 pKa = 4.12LVGEE1054 pKa = 4.44LAPGTTYY1061 pKa = 11.34NFTVEE1066 pKa = 4.59ASDD1069 pKa = 4.35GVNSTNSTEE1078 pKa = 4.09NFTTIYY1084 pKa = 10.56RR1085 pKa = 11.84PDD1087 pKa = 3.47EE1088 pKa = 4.62PYY1090 pKa = 11.28DD1091 pKa = 3.78PFPEE1095 pKa = 4.57NDD1097 pKa = 3.5TVIDD1101 pKa = 3.63TTTRR1105 pKa = 11.84SGDD1108 pKa = 3.78DD1109 pKa = 2.76IAASVKK1115 pKa = 9.69VVQDD1119 pKa = 4.16DD1120 pKa = 4.05GHH1122 pKa = 5.71TMNVQFINASDD1133 pKa = 4.24DD1134 pKa = 3.91SPLGTDD1140 pKa = 3.57YY1141 pKa = 11.32DD1142 pKa = 4.22VEE1144 pKa = 4.65SGTRR1148 pKa = 11.84AKK1150 pKa = 9.56LTSIGNTLRR1159 pKa = 11.84DD1160 pKa = 3.93EE1161 pKa = 4.6TNTTYY1166 pKa = 10.71RR1167 pKa = 11.84WYY1169 pKa = 10.67AVATDD1174 pKa = 3.59QTTGEE1179 pKa = 4.45SVRR1182 pKa = 11.84SDD1184 pKa = 2.99TFVFRR1189 pKa = 11.84TPEE1192 pKa = 3.8VGEE1195 pKa = 3.94VRR1197 pKa = 11.84FDD1199 pKa = 3.55VMQGRR1204 pKa = 11.84NEE1206 pKa = 3.94NMDD1209 pKa = 2.87ITGNSDD1215 pKa = 2.44NGYY1218 pKa = 9.73RR1219 pKa = 11.84KK1220 pKa = 9.52IKK1222 pKa = 10.74FEE1224 pKa = 4.17VTSTEE1229 pKa = 3.78MDD1231 pKa = 5.2PIPTVIVNEE1240 pKa = 4.28TGTGNMIKK1248 pKa = 9.81SWSNVANNSVLTIDD1262 pKa = 4.69LVQDD1266 pKa = 4.74GSQDD1270 pKa = 3.19WNLDD1274 pKa = 3.16TDD1276 pKa = 4.68EE1277 pKa = 5.04KK1278 pKa = 11.48YY1279 pKa = 10.67EE1280 pKa = 4.07WTVEE1284 pKa = 3.88ARR1286 pKa = 11.84DD1287 pKa = 3.71GSNILGNRR1295 pKa = 11.84SYY1297 pKa = 11.24DD1298 pKa = 2.87IYY1300 pKa = 10.53TYY1302 pKa = 11.28NVTLDD1307 pKa = 2.89WDD1309 pKa = 4.01RR1310 pKa = 11.84AEE1312 pKa = 4.66KK1313 pKa = 10.54YY1314 pKa = 10.52YY1315 pKa = 11.19NVYY1318 pKa = 10.43QYY1320 pKa = 11.12DD1321 pKa = 3.58IYY1323 pKa = 10.84RR1324 pKa = 11.84AEE1326 pKa = 3.95QRR1328 pKa = 11.84SGVDD1332 pKa = 3.09LTFNYY1337 pKa = 10.57GNGDD1341 pKa = 3.77YY1342 pKa = 10.92NLVGSLPEE1350 pKa = 3.85TSFNDD1355 pKa = 3.46SGPGIVPDD1363 pKa = 3.89HH1364 pKa = 6.77TGTFCWKK1371 pKa = 9.76VAASNPTGSSPAIPSGEE1388 pKa = 4.33GNCKK1392 pKa = 8.47TLNN1395 pKa = 3.45

Molecular weight: 148.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N0NTP7|A0A6N0NTP7_9EURY TetR family transcriptional regulator OS=Nanohaloarchaea archaeon OX=2594798 GN=GKQ38_03365 PE=4 SV=1
MM1 pKa = 7.8LGILLLLMIAVPFTDD16 pKa = 5.9LYY18 pKa = 10.89ILLQIAGMIGFWQTLAIVIATGVIGAAIVRR48 pKa = 11.84RR49 pKa = 11.84EE50 pKa = 3.8GRR52 pKa = 11.84HH53 pKa = 4.42VLKK56 pKa = 10.65KK57 pKa = 10.01LQRR60 pKa = 11.84SVTAGEE66 pKa = 4.07VSRR69 pKa = 11.84NVAEE73 pKa = 4.51GALLVIAGLLLITPGIITDD92 pKa = 3.43VTGFLLTFRR101 pKa = 11.84PLRR104 pKa = 11.84EE105 pKa = 3.61RR106 pKa = 11.84LVARR110 pKa = 11.84WISGNSGNIEE120 pKa = 3.74VEE122 pKa = 4.11VFRR125 pKa = 11.84FF126 pKa = 3.43

Molecular weight: 13.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1074

0

1074

277001

45

1395

257.9

28.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.619 ± 0.075

0.608 ± 0.023

7.122 ± 0.079

11.233 ± 0.175

3.928 ± 0.061

7.204 ± 0.079

1.676 ± 0.038

6.087 ± 0.064

5.834 ± 0.081

8.324 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.039

4.107 ± 0.066

3.404 ± 0.041

3.491 ± 0.062

4.672 ± 0.059

6.838 ± 0.11

5.089 ± 0.09

7.062 ± 0.055

0.916 ± 0.028

3.319 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski