Croceibacter atlanticus (strain ATCC BAA-628 / HTCC2559 / KCTC 12090)
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2702 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A3U7L0|A3U7L0_CROAH 30S ribosomal protein S12 OS=Croceibacter atlanticus (strain ATCC BAA-628 / HTCC2559 / KCTC 12090) OX=216432 GN=CA2559_05690 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.34 KK3 pKa = 10.14 IIYY6 pKa = 10.18 LFTLILAVGTFQSCEE21 pKa = 3.94 DD22 pKa = 3.83 YY23 pKa = 11.6 LDD25 pKa = 4.39 INDD28 pKa = 4.98 PLNDD32 pKa = 4.96 PIEE35 pKa = 4.56 SQVTPDD41 pKa = 5.01 LILAGAMTGSFPSQSNNMNRR61 pKa = 11.84 LGNVMMNNWAGNINAFTGGFNEE83 pKa = 4.63 EE84 pKa = 3.95 YY85 pKa = 10.58 QLIITSTFYY94 pKa = 11.32 NFIWDD99 pKa = 4.85 GIYY102 pKa = 10.86 LNVANFQAMIDD113 pKa = 3.56 ADD115 pKa = 3.79 FEE117 pKa = 4.42 NYY119 pKa = 9.02 EE120 pKa = 3.97 NHH122 pKa = 6.64 KK123 pKa = 10.82 AIAKK127 pKa = 9.54 IMKK130 pKa = 9.04 SYY132 pKa = 10.02 YY133 pKa = 7.49 MQYY136 pKa = 10.88 VVDD139 pKa = 5.42 LYY141 pKa = 11.71 GDD143 pKa = 3.67 APYY146 pKa = 10.53 TEE148 pKa = 5.15 AFQGGDD154 pKa = 3.84 NPTPTYY160 pKa = 10.93 DD161 pKa = 4.61 DD162 pKa = 3.84 DD163 pKa = 4.14 MEE165 pKa = 4.96 IYY167 pKa = 10.1 RR168 pKa = 11.84 ALITEE173 pKa = 3.9 VDD175 pKa = 3.54 EE176 pKa = 5.09 ALALIDD182 pKa = 4.89 NNTDD186 pKa = 2.88 LTNPVGSEE194 pKa = 3.64 DD195 pKa = 3.53 VVYY198 pKa = 10.8 NGNMTNWVTFANTLKK213 pKa = 10.73 LRR215 pKa = 11.84 ILMRR219 pKa = 11.84 QSTLAEE225 pKa = 4.2 TDD227 pKa = 3.67 GEE229 pKa = 4.58 TASYY233 pKa = 10.7 LATQFQALQDD243 pKa = 3.5 ASFITTTAAINPGYY257 pKa = 11.13 ANDD260 pKa = 4.05 TGRR263 pKa = 11.84 QNPFYY268 pKa = 9.94 ATYY271 pKa = 10.36 GFNVDD276 pKa = 4.05 GSQTTSNRR284 pKa = 11.84 FIVASLYY291 pKa = 10.85 AEE293 pKa = 4.22 QYY295 pKa = 11.4 LDD297 pKa = 3.55 GTLTGILDD305 pKa = 3.48 PRR307 pKa = 11.84 INEE310 pKa = 4.42 LYY312 pKa = 9.88 TPVDD316 pKa = 3.63 GEE318 pKa = 4.5 VQGVQQGVDD327 pKa = 3.58 SNDD330 pKa = 3.06 PSVPDD335 pKa = 4.2 EE336 pKa = 4.25 ISPLGEE342 pKa = 4.12 GLLVDD347 pKa = 4.55 ASQDD351 pKa = 3.27 GVLFSAAEE359 pKa = 4.17 SFFLQSEE366 pKa = 4.51 AVFRR370 pKa = 11.84 GYY372 pKa = 10.14 IAGDD376 pKa = 3.34 AKK378 pKa = 11.25 GLFQDD383 pKa = 5.5 GIRR386 pKa = 11.84 SSFDD390 pKa = 2.96 QLGIGAQAEE399 pKa = 4.38 AYY401 pKa = 7.1 ITNSDD406 pKa = 3.59 GVDD409 pKa = 3.43 EE410 pKa = 4.95 IGWDD414 pKa = 3.56 GSANKK419 pKa = 9.76 IEE421 pKa = 5.77 AIMTQKK427 pKa = 10.27 WIATNGINAIEE438 pKa = 4.46 SFIDD442 pKa = 3.34 MNRR445 pKa = 11.84 TNFPEE450 pKa = 4.07 VPLAINAQRR459 pKa = 11.84 DD460 pKa = 3.93 KK461 pKa = 11.4 KK462 pKa = 9.18 PTRR465 pKa = 11.84 LLYY468 pKa = 10.02 PSSEE472 pKa = 4.54 SIANAANKK480 pKa = 8.24 PAQEE484 pKa = 4.04 TDD486 pKa = 3.39 DD487 pKa = 4.5 AFNTYY492 pKa = 9.99 IFWDD496 pKa = 3.68 STQNN500 pKa = 3.24
Molecular weight: 55.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.605
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.948
Patrickios 1.367
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A3UAT9|A3UAT9_CROAH Uncharacterized protein OS=Croceibacter atlanticus (strain ATCC BAA-628 / HTCC2559 / KCTC 12090) OX=216432 GN=CA2559_12833 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.05 RR21 pKa = 11.84 MASVNGRR28 pKa = 11.84 KK29 pKa = 9.21 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.65 LSVSSEE47 pKa = 3.83 MRR49 pKa = 11.84 HH50 pKa = 4.65 KK51 pKa = 10.4 HH52 pKa = 4.85
Molecular weight: 6.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2702
0
2702
907128
20
8918
335.7
37.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.491 ± 0.042
0.725 ± 0.017
5.93 ± 0.094
6.615 ± 0.046
5.045 ± 0.05
6.362 ± 0.067
1.727 ± 0.025
7.63 ± 0.047
7.315 ± 0.094
9.284 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.079 ± 0.028
6.264 ± 0.052
3.365 ± 0.032
3.469 ± 0.03
3.355 ± 0.047
6.565 ± 0.048
6.446 ± 0.111
6.427 ± 0.05
0.954 ± 0.016
3.952 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here