Ajellomyces capsulatus (strain NAm1 / WU24) (Darling s disease fungus) (Histoplasma capsulatum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6RHA6|A6RHA6_AJECN Uncharacterized protein OS=Ajellomyces capsulatus (strain NAm1 / WU24) OX=339724 GN=HCAG_09023 PE=4 SV=1
MM1 pKa = 7.42SGYY4 pKa = 10.17SGRR7 pKa = 11.84RR8 pKa = 11.84APNFSQYY15 pKa = 11.36LNDD18 pKa = 4.46LNTIPSPYY26 pKa = 10.25DD27 pKa = 2.99QALQEE32 pKa = 4.16QEE34 pKa = 4.04QQQTLFDD41 pKa = 3.86VDD43 pKa = 4.1AEE45 pKa = 4.31LALFTNAEE53 pKa = 4.16FLDD56 pKa = 4.07FDD58 pKa = 4.59PVGEE62 pKa = 4.58LPVEE66 pKa = 4.06IPVKK70 pKa = 10.87YY71 pKa = 8.07EE72 pKa = 3.64TAEE75 pKa = 4.2GSEE78 pKa = 4.28GTDD81 pKa = 3.16MRR83 pKa = 11.84NSPDD87 pKa = 3.04TTTPISAGGDD97 pKa = 3.29NVKK100 pKa = 10.78YY101 pKa = 10.58IDD103 pKa = 4.07MLNGGWANLQFFASSPCCQLCPISSVRR130 pKa = 3.53

Molecular weight: 14.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6R092|A6R092_AJECN MaoC-like domain-containing protein OS=Ajellomyces capsulatus (strain NAm1 / WU24) OX=339724 GN=HCAG_03049 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9252

0

9252

4057025

30

4863

438.5

48.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.959 ± 0.021

1.279 ± 0.009

5.633 ± 0.016

6.326 ± 0.023

3.576 ± 0.015

6.634 ± 0.023

2.526 ± 0.012

5.124 ± 0.016

5.119 ± 0.02

8.804 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.008

3.936 ± 0.015

6.087 ± 0.025

4.159 ± 0.018

6.568 ± 0.023

8.428 ± 0.029

5.863 ± 0.016

5.811 ± 0.016

1.375 ± 0.009

2.674 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski