Pelagibacter phage HTVC010P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1HLJ3|M1HLJ3_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC010P OX=1283077 PE=4 SV=1
MM1 pKa = 7.3TFSNSTSDD9 pKa = 3.66ADD11 pKa = 3.76PGAGKK16 pKa = 9.97IAFNNGTLSSVSILYY31 pKa = 10.48VDD33 pKa = 4.79DD34 pKa = 6.02ADD36 pKa = 5.17DD37 pKa = 4.06ASADD41 pKa = 3.06ISSFVQSWDD50 pKa = 3.38DD51 pKa = 3.57VTNTTARR58 pKa = 11.84GIVTVTKK65 pKa = 10.05EE66 pKa = 4.35GTPSTYY72 pKa = 11.41ALFKK76 pKa = 11.01VSGAVTDD83 pKa = 3.72ASGYY87 pKa = 7.73TKK89 pKa = 10.7VPVTHH94 pKa = 6.01VVSSGTFSNADD105 pKa = 2.98GVGVHH110 pKa = 6.32FSYY113 pKa = 10.8SGADD117 pKa = 3.45GSDD120 pKa = 3.33GEE122 pKa = 4.61MTSFTLAGSSGSSQTITNGNTVTIAAGDD150 pKa = 4.53GITTTGGSTDD160 pKa = 3.36TVTIAVTDD168 pKa = 4.22DD169 pKa = 3.35PTALAIALGG178 pKa = 3.76

Molecular weight: 17.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1HLK9|M1HLK9_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC010P OX=1283077 PE=4 SV=1
MM1 pKa = 8.1AIRR4 pKa = 11.84KK5 pKa = 4.94TTKK8 pKa = 10.22GKK10 pKa = 9.27NANYY14 pKa = 10.36RR15 pKa = 11.84PTKK18 pKa = 10.07KK19 pKa = 10.39GAGMTAKK26 pKa = 10.37GVARR30 pKa = 11.84YY31 pKa = 9.19RR32 pKa = 11.84RR33 pKa = 11.84ANPGSKK39 pKa = 10.33LKK41 pKa = 9.83TAVTKK46 pKa = 10.79KK47 pKa = 10.34SGLTARR53 pKa = 11.84EE54 pKa = 3.51KK55 pKa = 10.87ARR57 pKa = 11.84RR58 pKa = 11.84KK59 pKa = 9.83SYY61 pKa = 10.09CARR64 pKa = 11.84SAGQLRR70 pKa = 11.84RR71 pKa = 11.84SSAKK75 pKa = 9.03TRR77 pKa = 11.84NDD79 pKa = 3.22PNSRR83 pKa = 11.84IRR85 pKa = 11.84QARR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84WRR92 pKa = 11.84CC93 pKa = 2.82

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

10379

42

854

162.2

18.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.303 ± 0.376

0.838 ± 0.133

6.465 ± 0.245

5.8 ± 0.429

4.316 ± 0.281

6.012 ± 0.377

1.561 ± 0.162

6.985 ± 0.259

8.141 ± 0.605

7.284 ± 0.393

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.172

6.176 ± 0.266

3.189 ± 0.235

4.268 ± 0.346

4.124 ± 0.325

6.773 ± 0.491

7.66 ± 0.776

5.55 ± 0.3

0.858 ± 0.106

4.076 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski