Coprobacillus sp. CAG:698
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1615 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5FWU4|R5FWU4_9FIRM CAAX amino terminal protease family protein OS=Coprobacillus sp. CAG:698 OX=1262856 GN=BN756_01383 PE=4 SV=1
MM1 pKa = 7.08 FVKK4 pKa = 10.41 IFDD7 pKa = 4.15 EE8 pKa = 3.99 NHH10 pKa = 6.06 EE11 pKa = 4.46 EE12 pKa = 4.09 DD13 pKa = 6.1 LEE15 pKa = 4.46 DD16 pKa = 4.81 AVNDD20 pKa = 4.83 FINNDD25 pKa = 4.1 GIKK28 pKa = 10.75 VIDD31 pKa = 3.97 IKK33 pKa = 11.09 FQVAIMYY40 pKa = 8.98 DD41 pKa = 3.49 NKK43 pKa = 11.1 DD44 pKa = 3.23 QIYY47 pKa = 9.31 CYY49 pKa = 9.55 TAMIIYY55 pKa = 9.94 DD56 pKa = 3.6 IEE58 pKa = 4.06 EE59 pKa = 3.88
Molecular weight: 7.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.91
Dawson 3.77
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.897
Patrickios 1.888
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|R5G4J3|R5G4J3_9FIRM ABC transporter domain-containing protein OS=Coprobacillus sp. CAG:698 OX=1262856 GN=BN756_00713 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.24 QPNKK9 pKa = 8.37 RR10 pKa = 11.84 KK11 pKa = 9.68 HH12 pKa = 6.01 SKK14 pKa = 7.21 THH16 pKa = 5.41 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATTNGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.37 GRR39 pKa = 11.84 KK40 pKa = 8.85 VLTAA44 pKa = 4.27
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.389
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.31
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.047
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.016
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1615
0
1615
557781
31
2472
345.4
39.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.727 ± 0.067
1.422 ± 0.025
5.876 ± 0.048
7.396 ± 0.061
4.557 ± 0.051
5.653 ± 0.066
1.275 ± 0.023
9.792 ± 0.081
9.55 ± 0.06
9.05 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.328 ± 0.031
6.827 ± 0.07
2.471 ± 0.035
2.105 ± 0.022
2.974 ± 0.04
6.316 ± 0.042
5.361 ± 0.056
6.463 ± 0.052
0.608 ± 0.019
5.249 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here