Wuhan spider virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJ44|A0A1L3KJ44_9VIRU Uncharacterized protein OS=Wuhan spider virus 4 OX=1923753 PE=4 SV=1
MM1 pKa = 7.5SKK3 pKa = 9.48MGAEE7 pKa = 4.59CSNAPAGDD15 pKa = 3.66CQFYY19 pKa = 10.77KK20 pKa = 10.99GVLNTYY26 pKa = 9.96AQIIRR31 pKa = 11.84QILEE35 pKa = 3.51QLEE38 pKa = 4.33AYY40 pKa = 9.9KK41 pKa = 10.63DD42 pKa = 4.0FPYY45 pKa = 10.5KK46 pKa = 10.97ANDD49 pKa = 3.62YY50 pKa = 10.55YY51 pKa = 12.16VMLTQVIISASDD63 pKa = 3.51DD64 pKa = 4.22DD65 pKa = 4.96FDD67 pKa = 4.16NCRR70 pKa = 11.84ALLASMDD77 pKa = 3.38SWIEE81 pKa = 3.89EE82 pKa = 3.72ISPFDD87 pKa = 3.53ARR89 pKa = 11.84DD90 pKa = 3.66VISRR94 pKa = 11.84TLMQALSKK102 pKa = 10.25FHH104 pKa = 7.24FSFKK108 pKa = 10.79QNLIDD113 pKa = 4.14NLKK116 pKa = 9.66KK117 pKa = 10.52ACPKK121 pKa = 10.09KK122 pKa = 10.78VRR124 pKa = 11.84TPLHH128 pKa = 5.65SQCSSCDD135 pKa = 3.38HH136 pKa = 6.4FPEE139 pKa = 5.75KK140 pKa = 10.61SEE142 pKa = 4.24EE143 pKa = 4.03EE144 pKa = 4.41VEE146 pKa = 4.5VVRR149 pKa = 11.84NAIYY153 pKa = 10.66EE154 pKa = 4.28MYY156 pKa = 10.92LEE158 pKa = 4.74DD159 pKa = 4.07QDD161 pKa = 3.95LKK163 pKa = 11.18RR164 pKa = 11.84RR165 pKa = 11.84GVLTRR170 pKa = 11.84KK171 pKa = 9.54PPSHH175 pKa = 6.65SFGGEE180 pKa = 4.04TIFKK184 pKa = 9.07PVPIPAPVHH193 pKa = 5.75KK194 pKa = 10.21SPPKK198 pKa = 8.05TPEE201 pKa = 3.63RR202 pKa = 11.84RR203 pKa = 11.84PSRR206 pKa = 11.84PSSRR210 pKa = 11.84NSQKK214 pKa = 10.46VYY216 pKa = 10.51RR217 pKa = 11.84CDD219 pKa = 3.25IPCYY223 pKa = 8.15PTQEE227 pKa = 4.27EE228 pKa = 4.2ANRR231 pKa = 11.84AYY233 pKa = 10.63RR234 pKa = 11.84KK235 pKa = 9.12AQHH238 pKa = 6.64DD239 pKa = 3.18AWFYY243 pKa = 11.21KK244 pKa = 9.98HH245 pKa = 6.43EE246 pKa = 4.16EE247 pKa = 3.99WPMEE251 pKa = 3.66ICEE254 pKa = 4.15PQIGEE259 pKa = 4.25PEE261 pKa = 4.46VYY263 pKa = 10.2DD264 pKa = 4.79QEE266 pKa = 5.57QDD268 pKa = 4.21DD269 pKa = 4.04SWRR272 pKa = 11.84DD273 pKa = 3.32PNVYY277 pKa = 10.52DD278 pKa = 5.25DD279 pKa = 5.64DD280 pKa = 4.13GTPFHH285 pKa = 6.96PRR287 pKa = 11.84NLPP290 pKa = 3.34

Molecular weight: 33.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJB8|A0A1L3KJB8_9VIRU Calici_coat domain-containing protein OS=Wuhan spider virus 4 OX=1923753 PE=4 SV=1
MM1 pKa = 7.37AAAALAAAVGGSTISAGATLGASLGAAQIQRR32 pKa = 11.84NTSLQVQDD40 pKa = 3.87SQQKK44 pKa = 7.33FTEE47 pKa = 4.89GIINRR52 pKa = 11.84GEE54 pKa = 3.81QAFTKK59 pKa = 10.47NGLPGYY65 pKa = 9.49LYY67 pKa = 9.86WQGTGPMPNTLYY79 pKa = 10.47QVNGANFAEE88 pKa = 4.74GFGVNTNLPYY98 pKa = 9.58YY99 pKa = 8.99TSNPMSQIYY108 pKa = 9.84KK109 pKa = 10.01AGKK112 pKa = 7.62PQYY115 pKa = 9.45SNKK118 pKa = 10.1DD119 pKa = 3.28ATEE122 pKa = 4.09TPPTKK127 pKa = 9.91ISSSPQSNTQPKK139 pKa = 8.25TFEE142 pKa = 4.41PPSEE146 pKa = 4.15FNRR149 pKa = 11.84TGWQSDD155 pKa = 3.44RR156 pKa = 11.84AGLGQGRR163 pKa = 11.84FASNYY168 pKa = 9.24NAVPPPNFNSAATQTRR184 pKa = 11.84NDD186 pKa = 3.35ALIRR190 pKa = 11.84SVGVQFPGPGPRR202 pKa = 11.84IITPRR207 pKa = 11.84VPSSYY212 pKa = 10.47RR213 pKa = 11.84AINN216 pKa = 3.2

Molecular weight: 22.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3749

216

2628

937.3

106.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.428 ± 0.738

1.867 ± 0.531

5.521 ± 0.601

6.082 ± 1.002

4.615 ± 0.535

5.361 ± 0.881

2.214 ± 0.395

5.788 ± 0.63

6.722 ± 1.047

7.709 ± 0.803

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.136

5.308 ± 0.55

5.628 ± 1.485

4.481 ± 0.702

4.428 ± 0.359

7.495 ± 0.663

5.601 ± 0.496

6.828 ± 0.658

1.654 ± 0.155

4.161 ± 0.547
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski