Wuhan spider virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJB2|A0A1L3KJB2_9VIRU Calici_coat domain-containing protein OS=Wuhan spider virus 6 OX=1923755 PE=4 SV=1
MM1 pKa = 7.36GFIMANISARR11 pKa = 11.84LFGDD15 pKa = 4.3EE16 pKa = 3.96IVEE19 pKa = 4.13MAEE22 pKa = 4.81RR23 pKa = 11.84RR24 pKa = 11.84AQEE27 pKa = 3.45NDD29 pKa = 2.74RR30 pKa = 11.84FGNQDD35 pKa = 3.49EE36 pKa = 4.71FPGYY40 pKa = 9.07QANNSDD46 pKa = 3.84PDD48 pKa = 3.84ALLEE52 pKa = 4.41AVDD55 pKa = 5.54FSPEE59 pKa = 4.3SITTNDD65 pKa = 3.1SLQPIVQKK73 pKa = 11.28LEE75 pKa = 3.93DD76 pKa = 3.79VQTAIKK82 pKa = 10.6DD83 pKa = 4.23LINSEE88 pKa = 4.23AVDD91 pKa = 3.59GRR93 pKa = 11.84MLEE96 pKa = 4.22DD97 pKa = 4.6LFTKK101 pKa = 10.22RR102 pKa = 11.84DD103 pKa = 3.59ALQGIMRR110 pKa = 11.84ASTFVRR116 pKa = 11.84MRR118 pKa = 11.84SAFPTMCAIGARR130 pKa = 11.84VSNDD134 pKa = 2.21ATTRR138 pKa = 11.84DD139 pKa = 3.74ACWALDD145 pKa = 3.32RR146 pKa = 11.84VLKK149 pKa = 10.96RR150 pKa = 11.84EE151 pKa = 3.77FTDD154 pKa = 4.02FFGSEE159 pKa = 4.26SNRR162 pKa = 11.84PYY164 pKa = 10.25PFPYY168 pKa = 10.04SRR170 pKa = 11.84FVDD173 pKa = 3.39IHH175 pKa = 6.01TLTLAKK181 pKa = 10.41ANNIFFQNHH190 pKa = 5.66PFAFGLKK197 pKa = 9.17YY198 pKa = 10.87YY199 pKa = 9.93QDD201 pKa = 3.74QIMAIARR208 pKa = 11.84NEE210 pKa = 3.76GNMRR214 pKa = 11.84KK215 pKa = 9.44RR216 pKa = 11.84KK217 pKa = 9.61HH218 pKa = 6.35KK219 pKa = 10.12ILNVLRR225 pKa = 11.84NCQSYY230 pKa = 9.82ISKK233 pKa = 10.82NYY235 pKa = 9.54FVYY238 pKa = 10.37KK239 pKa = 10.06GSPSIWYY246 pKa = 9.54DD247 pKa = 3.24DD248 pKa = 3.59GTEE251 pKa = 3.84VDD253 pKa = 5.93DD254 pKa = 5.76AIEE257 pKa = 3.86WLIDD261 pKa = 3.75FPEE264 pKa = 4.46VNSHH268 pKa = 5.53GFQMLATLPGGLNYY282 pKa = 10.53YY283 pKa = 7.99STYY286 pKa = 9.46WLSLRR291 pKa = 11.84AIALQNPVLRR301 pKa = 11.84ATIQPP306 pKa = 3.67

Molecular weight: 34.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJ37|A0A1L3KJ37_9VIRU Uncharacterized protein OS=Wuhan spider virus 6 OX=1923755 PE=4 SV=1
MM1 pKa = 7.4AQAQGLGLLINRR13 pKa = 11.84AADD16 pKa = 3.73YY17 pKa = 10.52QKK19 pKa = 11.11SAFDD23 pKa = 3.24TGLNYY28 pKa = 10.65LSNSALQNNFWNNKK42 pKa = 7.11QNYY45 pKa = 7.83QSNILNYY52 pKa = 10.72ANTLQRR58 pKa = 11.84SNFNYY63 pKa = 10.29LMDD66 pKa = 4.22MKK68 pKa = 10.7QDD70 pKa = 3.79SLQKK74 pKa = 10.67AGLPSYY80 pKa = 11.08LKK82 pKa = 10.69FSGNLPFGTPFSSQAMSGQNYY103 pKa = 10.06LNSTLPGNAARR114 pKa = 11.84APYY117 pKa = 9.82NAYY120 pKa = 8.71STQNLGWGQAPTYY133 pKa = 10.77

Molecular weight: 14.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3337

133

2329

834.3

94.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.593 ± 1.11

1.738 ± 0.382

5.634 ± 0.364

5.394 ± 0.891

5.634 ± 0.284

5.274 ± 0.442

2.068 ± 0.363

7.971 ± 1.062

6.922 ± 1.367

7.911 ± 0.484

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.279

6.233 ± 0.975

4.915 ± 0.231

4.195 ± 0.688

3.596 ± 0.698

7.312 ± 0.354

4.885 ± 0.603

5.814 ± 0.851

1.259 ± 0.055

4.046 ± 0.396
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski