Staphylococcus phage SH-St 15644

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus; Staphylococcus virus 15644

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VBU1|A0A2K9VBU1_9CAUD Uncharacterized protein OS=Staphylococcus phage SH-St 15644 OX=2066503 GN=SHSt15644_orf00031 PE=4 SV=1
MM1 pKa = 7.51NNRR4 pKa = 11.84EE5 pKa = 4.1QIEE8 pKa = 3.98QSVISASAYY17 pKa = 10.04NGNDD21 pKa = 3.29TEE23 pKa = 4.52GLLKK27 pKa = 10.49EE28 pKa = 4.44IEE30 pKa = 4.24DD31 pKa = 4.28VYY33 pKa = 11.57KK34 pKa = 10.64KK35 pKa = 10.72AQAFDD40 pKa = 4.61EE41 pKa = 4.37ILEE44 pKa = 4.31GMTNAIQHH52 pKa = 5.29SVKK55 pKa = 10.58EE56 pKa = 4.41GIEE59 pKa = 3.75LDD61 pKa = 3.71EE62 pKa = 4.98AVGIMTGQVVYY73 pKa = 10.18KK74 pKa = 10.68YY75 pKa = 11.02EE76 pKa = 3.83EE77 pKa = 4.11AQEE80 pKa = 3.91NDD82 pKa = 3.02

Molecular weight: 9.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VBU5|A0A2K9VBU5_9CAUD Uncharacterized protein OS=Staphylococcus phage SH-St 15644 OX=2066503 GN=SHSt15644_orf00048 PE=4 SV=1
MM1 pKa = 7.68NLMQHH6 pKa = 6.08WKK8 pKa = 7.96QTGLNPTARR17 pKa = 11.84KK18 pKa = 8.91EE19 pKa = 3.97RR20 pKa = 11.84GVVSFVSSMLHH31 pKa = 5.09HH32 pKa = 6.36QKK34 pKa = 11.01

Molecular weight: 3.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13937

34

2066

217.8

24.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.891 ± 0.62

0.509 ± 0.104

6.285 ± 0.27

7.792 ± 0.404

3.925 ± 0.245

6.085 ± 0.585

1.708 ± 0.11

7.103 ± 0.38

9.93 ± 0.482

8.287 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.116

6.407 ± 0.275

2.705 ± 0.208

3.666 ± 0.171

4.147 ± 0.219

6.314 ± 0.321

5.683 ± 0.243

5.575 ± 0.195

1.248 ± 0.129

4.233 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski