Pseudomonas phage JG012

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Nipunavirus; unclassified Nipunavirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4GY06|A0A2H4GY06_9CAUD Uncharacterized protein OS=Pseudomonas phage JG012 OX=1970799 GN=JG012_00033 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.28GLPRR6 pKa = 11.84STSRR10 pKa = 11.84GPLARR15 pKa = 11.84QDD17 pKa = 3.06IVKK20 pKa = 10.24QVVKK24 pKa = 10.64LNKK27 pKa = 10.21LPITVDD33 pKa = 2.92GATGVGFGTAVLGDD47 pKa = 3.96LPEE50 pKa = 4.8GNILFLGAVSYY61 pKa = 9.93VQIAKK66 pKa = 8.78ATAAGVQDD74 pKa = 4.57TFDD77 pKa = 3.71GDD79 pKa = 3.89YY80 pKa = 10.9SIGSAPTADD89 pKa = 3.39ATLSGSEE96 pKa = 3.89VDD98 pKa = 4.78IIASTALGAATAGVSPRR115 pKa = 11.84ARR117 pKa = 11.84GTNATQVILDD127 pKa = 3.84NTDD130 pKa = 3.11GSLEE134 pKa = 4.2LNLNLLIDD142 pKa = 4.65DD143 pKa = 4.98ANISADD149 pKa = 3.96SQSLTASGEE158 pKa = 3.85LHH160 pKa = 5.9IVYY163 pKa = 10.37SVLGDD168 pKa = 3.68DD169 pKa = 4.24

Molecular weight: 17.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4GY44|A0A2H4GY44_9CAUD DNA-directed DNA polymerase OS=Pseudomonas phage JG012 OX=1970799 GN=JG012_00030 PE=4 SV=1
MM1 pKa = 7.65IPRR4 pKa = 11.84FPMSPEE10 pKa = 3.49VAKK13 pKa = 10.91AIMEE17 pKa = 3.96EE18 pKa = 4.03HH19 pKa = 5.72EE20 pKa = 4.31RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 10.02ARR26 pKa = 11.84EE27 pKa = 3.31QRR29 pKa = 11.84KK30 pKa = 8.56RR31 pKa = 11.84AKK33 pKa = 10.37LLAKK37 pKa = 9.83EE38 pKa = 4.29LEE40 pKa = 4.04AALRR44 pKa = 11.84DD45 pKa = 4.03EE46 pKa = 4.77QGPAQPRR53 pKa = 11.84AHH55 pKa = 6.15WRR57 pKa = 11.84YY58 pKa = 8.23PYY60 pKa = 10.63GIGAHH65 pKa = 7.14AIALCALAAAAALGYY80 pKa = 9.65WLTHH84 pKa = 5.41

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

18269

43

976

237.3

26.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.646 ± 0.367

1.259 ± 0.12

5.742 ± 0.177

6.092 ± 0.297

3.547 ± 0.174

7.647 ± 0.29

1.916 ± 0.17

5.025 ± 0.163

5.041 ± 0.25

8.194 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.141

3.947 ± 0.156

4.538 ± 0.252

4.658 ± 0.346

5.944 ± 0.193

5.583 ± 0.267

5.89 ± 0.307

6.859 ± 0.213

1.779 ± 0.13

2.939 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski