Anaerorhabdus furcosa
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2330 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4PSL3|A0A1T4PSL3_9FIRM Uncharacterized protein OS=Anaerorhabdus furcosa OX=118967 GN=SAMN02745191_2128 PE=4 SV=1
MM1 pKa = 7.1 IQDD4 pKa = 3.64 IFKK7 pKa = 10.91 SFIVLGSCFLLSGCMQNQVNQDD29 pKa = 3.63 LNVEE33 pKa = 4.25 EE34 pKa = 4.36 NQPEE38 pKa = 4.21 EE39 pKa = 4.12 FNEE42 pKa = 4.52 GVEE45 pKa = 4.25 QMMITATINEE55 pKa = 4.18 IEE57 pKa = 4.21 YY58 pKa = 10.6 EE59 pKa = 4.82 IILEE63 pKa = 4.37 DD64 pKa = 3.88 NNTTRR69 pKa = 11.84 AFIDD73 pKa = 4.16 LLPLTIQMDD82 pKa = 3.96 DD83 pKa = 3.4 LNNNEE88 pKa = 4.28 KK89 pKa = 10.8 YY90 pKa = 10.43 FYY92 pKa = 10.81 LDD94 pKa = 2.94 AALPSDD100 pKa = 4.11 SYY102 pKa = 11.77 NPEE105 pKa = 4.17 TIQPGDD111 pKa = 3.3 FMLYY115 pKa = 10.66 GNNCIVLFYY124 pKa = 11.07 KK125 pKa = 10.55 SFQSNFSYY133 pKa = 10.73 TKK135 pKa = 10.64 LGTIKK140 pKa = 9.77 NTDD143 pKa = 3.4 GLQVIAASTSIEE155 pKa = 4.06 VTFAKK160 pKa = 10.6
Molecular weight: 18.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.859
IPC_protein 3.795
Toseland 3.605
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.63
Grimsley 3.516
Solomon 3.757
Lehninger 3.706
Nozaki 3.897
DTASelect 4.05
Thurlkill 3.656
EMBOSS 3.694
Sillero 3.91
Patrickios 0.604
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A1T4KSC1|A0A1T4KSC1_9FIRM 7 8-dihydropterin-6-yl-methyl-4-(Beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase OS=Anaerorhabdus furcosa OX=118967 GN=SAMN02745191_0682 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSKK9 pKa = 9.95 RR10 pKa = 11.84 KK11 pKa = 7.81 HH12 pKa = 5.11 QKK14 pKa = 8.48 VHH16 pKa = 5.53 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.82 TVGGRR28 pKa = 11.84 KK29 pKa = 8.55 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.63 GRR39 pKa = 11.84 KK40 pKa = 8.49 ILSAA44 pKa = 4.02
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2330
0
2330
723749
38
3129
310.6
35.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.77 ± 0.055
1.413 ± 0.019
5.544 ± 0.038
7.14 ± 0.069
4.711 ± 0.044
6.006 ± 0.059
1.736 ± 0.023
9.175 ± 0.043
7.672 ± 0.051
9.447 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.824 ± 0.024
5.634 ± 0.048
2.915 ± 0.029
3.283 ± 0.04
3.259 ± 0.03
6.214 ± 0.036
5.479 ± 0.051
6.775 ± 0.048
0.747 ± 0.014
4.256 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here