Gordonia phage Pollux

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KZ96|A0A345KZ96_9CAUD Tape measure protein OS=Gordonia phage Pollux OX=2250296 GN=18 PE=4 SV=1
MM1 pKa = 7.41AVAACLSLAVSAGCSSEE18 pKa = 4.26SGSNGATSAATSSAAASAPSVAITSWATWAQNYY51 pKa = 7.56QSEE54 pKa = 4.55IEE56 pKa = 4.28DD57 pKa = 3.93CAGEE61 pKa = 4.08TDD63 pKa = 3.65SCFTQNHH70 pKa = 4.41ATMAEE75 pKa = 4.07MRR77 pKa = 11.84QSIIDD82 pKa = 3.63NNLPIDD88 pKa = 3.97GTNSGIVNPMQLYY101 pKa = 9.65QEE103 pKa = 4.89TYY105 pKa = 11.4AKK107 pKa = 9.54FTEE110 pKa = 4.5QNCIGGPQDD119 pKa = 3.37MNSANVVCNVKK130 pKa = 9.84RR131 pKa = 11.84DD132 pKa = 3.52QMRR135 pKa = 11.84VFGSQVKK142 pKa = 9.38EE143 pKa = 4.12QLDD146 pKa = 3.8SMATGG151 pKa = 3.46

Molecular weight: 15.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KZ91|A0A345KZ91_9CAUD Tail terminator OS=Gordonia phage Pollux OX=2250296 GN=13 PE=4 SV=1
MM1 pKa = 7.63SVRR4 pKa = 11.84LRR6 pKa = 11.84FNIDD10 pKa = 2.36GFYY13 pKa = 10.89DD14 pKa = 3.72LRR16 pKa = 11.84RR17 pKa = 11.84DD18 pKa = 3.61PGIVAEE24 pKa = 4.75EE25 pKa = 3.86EE26 pKa = 4.87AIAQQIADD34 pKa = 4.32RR35 pKa = 11.84ANSIGKK41 pKa = 8.2GTYY44 pKa = 10.12AVGSRR49 pKa = 11.84QGRR52 pKa = 11.84KK53 pKa = 9.29APQGRR58 pKa = 11.84WRR60 pKa = 11.84TTVVTADD67 pKa = 3.27ARR69 pKa = 11.84AMANNARR76 pKa = 11.84NNTLIRR82 pKa = 11.84AMEE85 pKa = 3.98

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

16882

37

1884

205.9

22.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.013 ± 0.474

0.977 ± 0.142

6.8 ± 0.226

5.746 ± 0.286

2.666 ± 0.141

8.429 ± 0.518

2.186 ± 0.226

4.295 ± 0.182

3.116 ± 0.196

7.783 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.136

3.027 ± 0.212

5.598 ± 0.303

3.862 ± 0.35

7.736 ± 0.416

5.864 ± 0.296

6.889 ± 0.298

6.771 ± 0.24

2.038 ± 0.16

2.055 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski