Magnetospirillum sp. SS-4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Magnetospirillum; unclassified Magnetospirillum

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4587 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J4FYC0|A0A6J4FYC0_9PROT Uncharacterized protein OS=Magnetospirillum sp. SS-4 OX=2681465 GN=MTBSS4_460011 PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.22KK3 pKa = 10.07YY4 pKa = 10.92VCVVCNFVYY13 pKa = 10.57DD14 pKa = 4.08PAIGMPDD21 pKa = 3.47DD22 pKa = 5.66GIPAGTPFEE31 pKa = 5.25NIPDD35 pKa = 3.68DD36 pKa = 3.92WTCPEE41 pKa = 4.54CGVSKK46 pKa = 11.0SDD48 pKa = 4.46FEE50 pKa = 4.47VQEE53 pKa = 4.29GG54 pKa = 3.47

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J4F4X8|A0A6J4F4X8_9PROT Riboflavin biosynthesis protein OS=Magnetospirillum sp. SS-4 OX=2681465 GN=ribF PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VINNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84QGRR39 pKa = 11.84KK40 pKa = 9.21KK41 pKa = 10.63LSAA44 pKa = 3.91

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4587

0

4587

1402263

20

11740

305.7

33.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.678 ± 0.072

1.026 ± 0.018

5.996 ± 0.032

5.626 ± 0.041

3.419 ± 0.024

8.75 ± 0.051

2.243 ± 0.022

4.825 ± 0.027

3.158 ± 0.038

10.256 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.023

2.392 ± 0.029

5.295 ± 0.041

2.876 ± 0.021

7.732 ± 0.077

5.227 ± 0.043

5.01 ± 0.067

7.547 ± 0.031

1.351 ± 0.016

1.973 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski