Pseudomonas phage PaP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6PVJ5|Q6PVJ5_9CAUD p38 OS=Pseudomonas phage PaP2 OX=270673 GN=orf38 PE=4 SV=1
MM1 pKa = 8.04ADD3 pKa = 3.3FCKK6 pKa = 10.57ACSLAMWGEE15 pKa = 4.18DD16 pKa = 3.21FGEE19 pKa = 4.03LAGITKK25 pKa = 10.17QEE27 pKa = 3.85DD28 pKa = 3.29WDD30 pKa = 3.82NGKK33 pKa = 9.7ACNVICEE40 pKa = 4.5GCGVIQVDD48 pKa = 4.0PEE50 pKa = 4.74GEE52 pKa = 4.45CVSSDD57 pKa = 3.83CIKK60 pKa = 10.76AGKK63 pKa = 7.67TGHH66 pKa = 5.91GTKK69 pKa = 9.9AWNKK73 pKa = 8.73GTDD76 pKa = 3.09NDD78 pKa = 3.87

Molecular weight: 8.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6PVK6|Q6PVK6_9CAUD p11 OS=Pseudomonas phage PaP2 OX=270673 GN=orf11 PE=4 SV=1
MM1 pKa = 6.97QRR3 pKa = 11.84LAIISAHH10 pKa = 6.02EE11 pKa = 4.09SLAEE15 pKa = 4.03EE16 pKa = 4.76DD17 pKa = 4.21SSFLLSSMEE26 pKa = 3.68VHH28 pKa = 7.39AISARR33 pKa = 11.84LGNVAIACLSITSSRR48 pKa = 11.84SISQRR53 pKa = 11.84PGITEE58 pKa = 4.1DD59 pKa = 3.23LRR61 pKa = 11.84KK62 pKa = 9.82VRR64 pKa = 11.84HH65 pKa = 4.99VNRR68 pKa = 11.84LNATSSRR75 pKa = 11.84TLRR78 pKa = 11.84TFFKK82 pKa = 10.64RR83 pKa = 11.84RR84 pKa = 11.84ADD86 pKa = 3.68NNQLSIRR93 pKa = 11.84SDD95 pKa = 3.25DD96 pKa = 3.64SSRR99 pKa = 11.84DD100 pKa = 3.76TTSLTLDD107 pKa = 3.63GKK109 pKa = 10.5IEE111 pKa = 4.57AVDD114 pKa = 3.24QFQAHH119 pKa = 6.76ILASSLVIEE128 pKa = 4.62DD129 pKa = 3.4HH130 pKa = 6.4TFGKK134 pKa = 7.79TVRR137 pKa = 11.84VFYY140 pKa = 10.91SEE142 pKa = 4.08NSSISRR148 pKa = 11.84SFRR151 pKa = 11.84SYY153 pKa = 8.85YY154 pKa = 10.37CRR156 pKa = 11.84GKK158 pKa = 9.91YY159 pKa = 9.62HH160 pKa = 7.08GLRR163 pKa = 11.84KK164 pKa = 10.0VSMGRR169 pKa = 11.84SLSGSAANYY178 pKa = 9.28RR179 pKa = 11.84SHH181 pKa = 6.16SQQWCRR187 pKa = 11.84TIRR190 pKa = 11.84NSTKK194 pKa = 10.0NSPSKK199 pKa = 10.28GLHH202 pKa = 5.04VEE204 pKa = 4.31FLRR207 pKa = 11.84IQGGIKK213 pKa = 8.53NTSINAHH220 pKa = 6.78KK221 pKa = 10.6YY222 pKa = 9.69SPDD225 pKa = 3.39YY226 pKa = 10.48LKK228 pKa = 10.68KK229 pKa = 10.55YY230 pKa = 10.64SSS232 pKa = 3.43

Molecular weight: 26.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

14421

48

1849

248.6

27.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.453 ± 0.449

0.943 ± 0.178

5.478 ± 0.234

6.421 ± 0.313

4.147 ± 0.216

6.248 ± 0.336

1.359 ± 0.146

6.199 ± 0.264

5.95 ± 0.325

9.334 ± 0.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.15

5.0 ± 0.188

4.611 ± 0.322

4.792 ± 0.474

4.896 ± 0.263

6.837 ± 0.372

5.839 ± 0.316

5.617 ± 0.212

1.449 ± 0.156

3.599 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski