Pelagibacter phage HTVC008M

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 198 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1I7P3|M1I7P3_9CAUD Putative carbamoyltransferase OS=Pelagibacter phage HTVC008M OX=1283076 PE=3 SV=1
MM1 pKa = 7.18MCGEE5 pKa = 4.39STTVLTQIVNGFKK18 pKa = 9.98MKK20 pKa = 10.46SLAAGQVKK28 pKa = 10.08YY29 pKa = 10.87AGDD32 pKa = 4.03LDD34 pKa = 4.13GDD36 pKa = 4.44SIGVISFWIHH46 pKa = 5.06PTLEE50 pKa = 4.18YY51 pKa = 11.13GSMLMTIKK59 pKa = 10.43EE60 pKa = 4.3GNLTCLLGYY69 pKa = 10.34GVDD72 pKa = 4.01WQWDD76 pKa = 3.87TDD78 pKa = 3.86LMIDD82 pKa = 4.3VVNEE86 pKa = 3.97VINEE90 pKa = 4.27DD91 pKa = 3.64EE92 pKa = 4.57TSTQQ96 pKa = 3.09

Molecular weight: 10.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1IPH1|M1IPH1_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC008M OX=1283076 PE=4 SV=1
MM1 pKa = 7.78AIIYY5 pKa = 7.39THH7 pKa = 5.71NTSGAIRR14 pKa = 11.84RR15 pKa = 11.84LKK17 pKa = 10.11RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84PTKK23 pKa = 10.13EE24 pKa = 3.4YY25 pKa = 9.68MIALAKK31 pKa = 9.55HH32 pKa = 5.31IKK34 pKa = 9.07YY35 pKa = 10.06LKK37 pKa = 10.61KK38 pKa = 10.73LGLKK42 pKa = 10.45VNDD45 pKa = 3.71KK46 pKa = 10.97GRR48 pKa = 11.84IVMKK52 pKa = 10.07QNPKK56 pKa = 8.82YY57 pKa = 7.41TTVTYY62 pKa = 11.22NEE64 pKa = 3.86ISKK67 pKa = 10.93DD68 pKa = 3.31STKK71 pKa = 10.83RR72 pKa = 11.84LDD74 pKa = 3.11AEE76 pKa = 3.84YY77 pKa = 9.5WINKK81 pKa = 8.25KK82 pKa = 10.53FSGGTKK88 pKa = 9.64PVNNWRR94 pKa = 11.84LEE96 pKa = 3.86EE97 pKa = 4.32SKK99 pKa = 11.14NFTIAPAYY107 pKa = 10.42NKK109 pKa = 10.15GAYY112 pKa = 8.32QVITKK117 pKa = 10.68SNVKK121 pKa = 10.45DD122 pKa = 3.04IGKK125 pKa = 9.99

Molecular weight: 14.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

198

0

198

47059

38

2001

237.7

26.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.067 ± 0.244

0.759 ± 0.069

6.883 ± 0.144

6.335 ± 0.194

4.241 ± 0.104

6.643 ± 0.273

1.942 ± 0.133

6.968 ± 0.157

8.162 ± 0.353

7.429 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.122

6.109 ± 0.139

3.353 ± 0.103

3.553 ± 0.115

3.629 ± 0.143

6.335 ± 0.235

7.697 ± 0.481

6.101 ± 0.146

1.113 ± 0.089

4.057 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski