Bovine faeces associated smacovirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Bonzesmacovirus; Bonzesmacovirus bovas1

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160HX16|A0A160HX16_9VIRU Putative capsid protein OS=Bovine faeces associated smacovirus 1 OX=1843749 PE=4 SV=1
MM1 pKa = 7.73GFITEE6 pKa = 4.42DD7 pKa = 3.08AFFPEE12 pKa = 4.66FLEE15 pKa = 4.3KK16 pKa = 9.93TLQEE20 pKa = 4.46ALDD23 pKa = 4.54KK24 pKa = 10.92ICEE27 pKa = 3.98RR28 pKa = 11.84YY29 pKa = 10.13AYY31 pKa = 9.98GNEE34 pKa = 3.99VGKK37 pKa = 10.31DD38 pKa = 3.52GYY40 pKa = 10.23EE41 pKa = 3.89HH42 pKa = 5.92FQCRR46 pKa = 11.84IVCSKK51 pKa = 9.36PTDD54 pKa = 3.4EE55 pKa = 4.04RR56 pKa = 11.84ALRR59 pKa = 11.84VFLLSNGIPAHH70 pKa = 5.88TSPTQVRR77 pKa = 11.84NFEE80 pKa = 4.27YY81 pKa = 10.56VQKK84 pKa = 10.64EE85 pKa = 4.03GNLYY89 pKa = 10.38CSWEE93 pKa = 4.04AVLKK97 pKa = 9.03QFPRR101 pKa = 11.84GTPYY105 pKa = 9.25MWQLIAFSMWKK116 pKa = 9.32KK117 pKa = 10.5QNDD120 pKa = 3.61RR121 pKa = 11.84EE122 pKa = 4.25ILVITDD128 pKa = 2.97DD129 pKa = 3.75KK130 pKa = 11.4GRR132 pKa = 11.84HH133 pKa = 4.7GKK135 pKa = 8.87SWLRR139 pKa = 11.84KK140 pKa = 9.26YY141 pKa = 9.72MVACHH146 pKa = 6.36MGTFIPPLEE155 pKa = 4.17KK156 pKa = 10.98AEE158 pKa = 5.48DD159 pKa = 3.43IMACAMAKK167 pKa = 9.77PSKK170 pKa = 10.61GYY172 pKa = 10.2IIDD175 pKa = 3.59MPRR178 pKa = 11.84ADD180 pKa = 3.51GKK182 pKa = 10.0VRR184 pKa = 11.84KK185 pKa = 9.78AMWSAIEE192 pKa = 3.97QMKK195 pKa = 10.65DD196 pKa = 2.53GYY198 pKa = 11.02LYY200 pKa = 10.68DD201 pKa = 5.43KK202 pKa = 10.53RR203 pKa = 11.84YY204 pKa = 9.62SWQEE208 pKa = 3.41KK209 pKa = 8.89WIEE212 pKa = 3.85PPKK215 pKa = 10.91IMVFCNDD222 pKa = 4.26FDD224 pKa = 4.92PLMLSTDD231 pKa = 2.89RR232 pKa = 11.84WQSFDD237 pKa = 3.13ITDD240 pKa = 4.58FPQEE244 pKa = 3.71

Molecular weight: 28.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160HX16|A0A160HX16_9VIRU Putative capsid protein OS=Bovine faeces associated smacovirus 1 OX=1843749 PE=4 SV=1
MM1 pKa = 6.86PTVHH5 pKa = 7.6ISEE8 pKa = 4.5TFDD11 pKa = 3.96LSTKK15 pKa = 10.14AGKK18 pKa = 10.16LGLIGIHH25 pKa = 5.47TPPRR29 pKa = 11.84SLVEE33 pKa = 3.74LHH35 pKa = 6.37YY36 pKa = 10.96GPLMSNYY43 pKa = 9.96RR44 pKa = 11.84KK45 pKa = 9.57IRR47 pKa = 11.84FLGADD52 pKa = 3.39VVLACASTLPLDD64 pKa = 3.96PLKK67 pKa = 11.31VGDD70 pKa = 4.21GATLFNPADD79 pKa = 3.53AMNPILYY86 pKa = 9.9KK87 pKa = 10.63AVSNNSFEE95 pKa = 4.27TLVAKK100 pKa = 10.4IKK102 pKa = 10.66GALPVLPSSAGSSIDD117 pKa = 3.12RR118 pKa = 11.84TEE120 pKa = 4.89NIDD123 pKa = 3.72TSTTNYY129 pKa = 10.88DD130 pKa = 3.81DD131 pKa = 4.32FDD133 pKa = 4.09VYY135 pKa = 11.45YY136 pKa = 10.39GILSATFGWKK146 pKa = 10.05KK147 pKa = 11.02SMPQTGLVMRR157 pKa = 11.84NLRR160 pKa = 11.84PLMYY164 pKa = 9.9PLLSDD169 pKa = 3.57IGNDD173 pKa = 2.83IMSASGNIVQDD184 pKa = 3.38STISNNRR191 pKa = 11.84DD192 pKa = 2.97TTSYY196 pKa = 11.03SGIGRR201 pKa = 11.84IARR204 pKa = 11.84GKK206 pKa = 9.28AQRR209 pKa = 11.84APALPLWQGIQSNSGILSPIPSTFPTTYY237 pKa = 8.8VGAVIVPPCHH247 pKa = 6.25TNGSVTYY254 pKa = 9.45YY255 pKa = 10.34RR256 pKa = 11.84LRR258 pKa = 11.84ITWHH262 pKa = 5.47IRR264 pKa = 11.84FEE266 pKa = 4.36GLCPYY271 pKa = 9.57TEE273 pKa = 4.69HH274 pKa = 7.52SPFANFQMAGGATYY288 pKa = 9.09WADD291 pKa = 3.48YY292 pKa = 9.46TGSSKK297 pKa = 10.92DD298 pKa = 3.67FEE300 pKa = 5.58HH301 pKa = 7.89DD302 pKa = 3.35EE303 pKa = 4.59STLSTNGFEE312 pKa = 4.45IEE314 pKa = 4.2KK315 pKa = 10.77VMM317 pKa = 5.07

Molecular weight: 34.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

561

244

317

280.5

31.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.595 ± 0.291

1.783 ± 0.707

6.061 ± 0.59

4.991 ± 1.553

4.635 ± 0.718

6.952 ± 1.057

1.961 ± 0.209

6.595 ± 0.291

5.704 ± 1.622

7.665 ± 0.988

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.743 ± 0.765

3.922 ± 0.952

6.417 ± 0.442

3.03 ± 0.962

4.635 ± 0.451

7.487 ± 1.939

6.595 ± 1.625

4.635 ± 0.349

2.139 ± 0.742

4.456 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski