Jujube mosaic-associated virus
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A221C9G4|A0A221C9G4_9VIRU P4 OS=Jujube mosaic-associated virus OX=2020956 PE=4 SV=1
MM1 pKa = 7.76 ADD3 pKa = 3.34 AGRR6 pKa = 11.84 SGSSGPSPIVDD17 pKa = 3.72 PDD19 pKa = 3.34 QLTPLLWLAANYY31 pKa = 9.7 RR32 pKa = 11.84 DD33 pKa = 3.94 RR34 pKa = 11.84 LEE36 pKa = 4.83 DD37 pKa = 3.63 LSARR41 pKa = 11.84 PIAADD46 pKa = 3.88 KK47 pKa = 10.77 YY48 pKa = 10.5 DD49 pKa = 3.99 QIAQRR54 pKa = 11.84 ARR56 pKa = 11.84 ATHH59 pKa = 6.19 QIAKK63 pKa = 9.66 NAVLATLEE71 pKa = 4.13 EE72 pKa = 4.28 MSQVLEE78 pKa = 3.89 AQKK81 pKa = 11.28 NYY83 pKa = 10.29 FQMSAIKK90 pKa = 10.34 DD91 pKa = 3.51 NYY93 pKa = 10.8 YY94 pKa = 11.19 GDD96 pKa = 5.32 LYY98 pKa = 10.64 PLLKK102 pKa = 10.43 QNQEE106 pKa = 3.8 AFEE109 pKa = 4.16 KK110 pKa = 10.65 AKK112 pKa = 10.57 QAVEE116 pKa = 4.02 RR117 pKa = 11.84 TLEE120 pKa = 4.26 LDD122 pKa = 3.35 VSLL125 pKa = 5.1
Molecular weight: 13.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.928
IPC2_protein 5.003
IPC_protein 4.838
Toseland 4.698
ProMoST 4.965
Dawson 4.8
Bjellqvist 4.952
Wikipedia 4.685
Rodwell 4.685
Grimsley 4.609
Solomon 4.8
Lehninger 4.749
Nozaki 4.914
DTASelect 5.092
Thurlkill 4.698
EMBOSS 4.711
Sillero 4.965
Patrickios 4.151
IPC_peptide 4.8
IPC2_peptide 4.952
IPC2.peptide.svr19 4.977
Protein with the highest isoelectric point:
>tr|A0A221C9G3|A0A221C9G3_9VIRU P3a OS=Jujube mosaic-associated virus OX=2020956 PE=4 SV=1
MM1 pKa = 7.29 TSWAYY6 pKa = 8.58 TAEE9 pKa = 4.07 KK10 pKa = 10.15 QSYY13 pKa = 8.69 KK14 pKa = 10.75 DD15 pKa = 3.31 AVAATEE21 pKa = 4.49 SIEE24 pKa = 4.07 APAVGFARR32 pKa = 11.84 PAEE35 pKa = 4.12 YY36 pKa = 10.57 RR37 pKa = 11.84 NTLASLGAVTKK48 pKa = 10.5 QNNTILVILTEE59 pKa = 4.15 LTEE62 pKa = 4.38 KK63 pKa = 11.13 VNFLQLQVQQLQQQVLKK80 pKa = 11.02 GKK82 pKa = 10.36 APEE85 pKa = 4.08 NLEE88 pKa = 3.8 QSISDD93 pKa = 4.5 LSNKK97 pKa = 9.71 LAGLKK102 pKa = 10.16 VDD104 pKa = 4.6 GADD107 pKa = 3.6 SAIPKK112 pKa = 9.9 KK113 pKa = 10.68 KK114 pKa = 10.1 GVLYY118 pKa = 10.34 VYY120 pKa = 9.39 QKK122 pKa = 10.8 PSDD125 pKa = 3.27 ILDD128 pKa = 3.43 RR129 pKa = 11.84 EE130 pKa = 4.49 KK131 pKa = 11.11 ARR133 pKa = 11.84 KK134 pKa = 8.63
Molecular weight: 14.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.566
IPC2_protein 8.448
IPC_protein 8.448
Toseland 9.224
ProMoST 9.063
Dawson 9.443
Bjellqvist 9.077
Wikipedia 9.589
Rodwell 9.78
Grimsley 9.516
Solomon 9.545
Lehninger 9.531
Nozaki 9.136
DTASelect 9.092
Thurlkill 9.268
EMBOSS 9.619
Sillero 9.355
Patrickios 5.041
IPC_peptide 9.545
IPC2_peptide 7.527
IPC2.peptide.svr19 7.68
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2278
125
1597
455.6
51.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.585 ± 1.689
1.536 ± 0.757
6.541 ± 1.119
6.76 ± 0.995
3.029 ± 0.623
5.356 ± 0.892
2.371 ± 0.47
6.014 ± 0.569
6.102 ± 1.368
9.35 ± 1.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.502 ± 0.465
4.478 ± 0.436
5.575 ± 1.186
5.663 ± 0.76
5.312 ± 1.262
6.409 ± 0.908
5.97 ± 0.91
5.312 ± 0.824
1.449 ± 0.319
3.687 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here