Aeromonas phage LAh_7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514A090|A0A514A090_9CAUD Uncharacterized protein OS=Aeromonas phage LAh_7 OX=2591031 GN=LAh7_35 PE=4 SV=1
MM1 pKa = 5.98TTEE4 pKa = 3.52IKK6 pKa = 9.79TYY8 pKa = 10.13RR9 pKa = 11.84VTWAIDD15 pKa = 3.51LEE17 pKa = 4.66GTSAEE22 pKa = 4.23QVASLAARR30 pKa = 11.84DD31 pKa = 3.9YY32 pKa = 11.11LQAGHH37 pKa = 6.56TATVLEE43 pKa = 4.79VAQPWGDD50 pKa = 3.35AGLYY54 pKa = 10.05APAVSVDD61 pKa = 3.35VGQLPIARR69 pKa = 11.84PDD71 pKa = 3.33NALEE75 pKa = 4.59LLGQDD80 pKa = 3.46LEE82 pKa = 4.73EE83 pKa = 4.84YY84 pKa = 10.89GLTSDD89 pKa = 4.03WEE91 pKa = 4.27RR92 pKa = 11.84ADD94 pKa = 3.73VLAHH98 pKa = 6.77IANNLAALLPAAAVKK113 pKa = 10.28AAIDD117 pKa = 3.8CAFADD122 pKa = 4.48ALICLEE128 pKa = 4.66PSSSQDD134 pKa = 2.92KK135 pKa = 11.12ADD137 pKa = 3.54TLRR140 pKa = 11.84RR141 pKa = 11.84MMEE144 pKa = 4.09GQPP147 pKa = 3.37

Molecular weight: 15.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514A0C3|A0A514A0C3_9CAUD Uncharacterized protein OS=Aeromonas phage LAh_7 OX=2591031 GN=LAh7_69 PE=4 SV=1
MM1 pKa = 7.65ISRR4 pKa = 11.84SEE6 pKa = 3.85LLEE9 pKa = 3.98AVEE12 pKa = 4.22TGRR15 pKa = 11.84PYY17 pKa = 10.99LVAVAKK23 pKa = 10.51DD24 pKa = 3.52YY25 pKa = 11.34AGRR28 pKa = 11.84LAGEE32 pKa = 4.31VISRR36 pKa = 11.84HH37 pKa = 4.46RR38 pKa = 11.84TRR40 pKa = 11.84SGAEE44 pKa = 3.57RR45 pKa = 11.84KK46 pKa = 9.53QKK48 pKa = 7.78TRR50 pKa = 11.84RR51 pKa = 11.84GAWEE55 pKa = 3.98VVDD58 pKa = 4.24AARR61 pKa = 11.84QLRR64 pKa = 11.84IAA66 pKa = 4.37

Molecular weight: 7.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

19299

46

1472

257.3

28.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.234 ± 0.683

1.031 ± 0.128

6.607 ± 0.256

5.819 ± 0.3

3.073 ± 0.135

7.544 ± 0.281

1.824 ± 0.172

4.715 ± 0.148

4.627 ± 0.286

8.301 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.715 ± 0.126

3.306 ± 0.138

5.441 ± 0.399

4.0 ± 0.296

6.803 ± 0.231

5.213 ± 0.147

5.591 ± 0.243

7.052 ± 0.3

1.928 ± 0.17

2.99 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski