Capybara microvirus Cap3_SP_414
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5U7|A0A4P8W5U7_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_414 OX=2585447 PE=4 SV=1
MM1 pKa = 7.56 SNNKK5 pKa = 8.81 IFQAEE10 pKa = 4.13 IVEE13 pKa = 4.48 KK14 pKa = 10.11 EE15 pKa = 4.01 YY16 pKa = 10.94 QSRR19 pKa = 11.84 NEE21 pKa = 3.76 FDD23 pKa = 3.51 FTHH26 pKa = 7.52 DD27 pKa = 3.55 NLTTLDD33 pKa = 3.7 MGNIVPLDD41 pKa = 3.79 SVLLFPGDD49 pKa = 3.15 ICDD52 pKa = 4.0 ISSEE56 pKa = 4.88 TYY58 pKa = 10.15 FQTHH62 pKa = 6.0 PCITNLLSKK71 pKa = 9.51 VYY73 pKa = 10.31 CDD75 pKa = 3.93 EE76 pKa = 5.07 LDD78 pKa = 4.76 FIAPLSRR85 pKa = 11.84 VWTLWQDD92 pKa = 3.71 YY93 pKa = 9.94 ISPGNGTRR101 pKa = 11.84 PLSHH105 pKa = 7.12 TDD107 pKa = 3.23 PYY109 pKa = 10.12 RR110 pKa = 11.84 TPTKK114 pKa = 9.88 PYY116 pKa = 10.23 FVFEE120 pKa = 4.9 DD121 pKa = 4.05 IILASLQTSFSILNFDD137 pKa = 4.56 INFTYY142 pKa = 10.95 APMKK146 pKa = 9.89 QRR148 pKa = 11.84 QFMLIDD154 pKa = 3.57 YY155 pKa = 8.78 LHH157 pKa = 6.59 YY158 pKa = 10.12 PIQNFISRR166 pKa = 11.84 SDD168 pKa = 3.64 LDD170 pKa = 3.91 LSNLHH175 pKa = 5.92 TFRR178 pKa = 11.84 NYY180 pKa = 10.11 VKK182 pKa = 10.74 LSQTNQTPINLKK194 pKa = 10.54 RR195 pKa = 11.84 IGLTPNYY202 pKa = 9.61 YY203 pKa = 10.15 RR204 pKa = 11.84 GVANLLNDD212 pKa = 4.89 LLPTSLQNKK221 pKa = 9.18 IYY223 pKa = 9.32 IHH225 pKa = 5.13 QVYY228 pKa = 9.86 EE229 pKa = 4.21 EE230 pKa = 4.31 VGKK233 pKa = 10.94 VPYY236 pKa = 10.09 NAIIYY241 pKa = 10.4 LSDD244 pKa = 4.25 VINAWLYY251 pKa = 10.25 DD252 pKa = 3.72 LEE254 pKa = 5.98 IIFPVNYY261 pKa = 9.59 YY262 pKa = 10.75 NDD264 pKa = 3.57 VSSVPDD270 pKa = 3.87 FNEE273 pKa = 4.35 IILLSIIEE281 pKa = 4.33 CLANQKK287 pKa = 10.26 SDD289 pKa = 3.41 PLVYY293 pKa = 10.33 CNSDD297 pKa = 2.76 IYY299 pKa = 11.41 DD300 pKa = 3.99 PYY302 pKa = 11.49 HH303 pKa = 6.05 DD304 pKa = 4.41 VKK306 pKa = 11.44 YY307 pKa = 10.99 NDD309 pKa = 3.09 YY310 pKa = 11.3 SYY312 pKa = 11.41 FRR314 pKa = 11.84 NNNLNLVMYY323 pKa = 10.04 YY324 pKa = 10.65 CDD326 pKa = 3.9 FANSFPSEE334 pKa = 4.0 TKK336 pKa = 10.44 EE337 pKa = 3.65 ILKK340 pKa = 10.3 KK341 pKa = 10.42 CFYY344 pKa = 10.03 VAGGVRR350 pKa = 11.84 QTIYY354 pKa = 10.52 KK355 pKa = 9.84 VEE357 pKa = 4.22 PNEE360 pKa = 4.85 LDD362 pKa = 3.27 TTNYY366 pKa = 9.91 QVIDD370 pKa = 3.63 ALEE373 pKa = 4.41 FYY375 pKa = 10.57 AYY377 pKa = 10.82 LKK379 pKa = 10.04 VCCDD383 pKa = 3.37 YY384 pKa = 11.06 FRR386 pKa = 11.84 DD387 pKa = 3.62 QNYY390 pKa = 10.58 DD391 pKa = 3.04 ILEE394 pKa = 4.13 VLISEE399 pKa = 4.59 SVKK402 pKa = 11.09 SNLNSKK408 pKa = 9.84 FQLTQEE414 pKa = 5.16 GYY416 pKa = 10.26 LSPSSIPEE424 pKa = 4.2 LFVSCYY430 pKa = 9.97 KK431 pKa = 10.31 KK432 pKa = 10.72 DD433 pKa = 3.77 YY434 pKa = 8.15 FTTATEE440 pKa = 4.05 TAQRR444 pKa = 11.84 GNPIEE449 pKa = 4.61 IPTTYY454 pKa = 8.77 ITLPDD459 pKa = 3.89 PVIGGTASKK468 pKa = 11.06 VRR470 pKa = 11.84 MNVFDD475 pKa = 4.03 PQNPFVIFDD484 pKa = 3.93 NDD486 pKa = 3.21 STMKK490 pKa = 10.4 SLSISGTSQPLTVLDD505 pKa = 3.68 LRR507 pKa = 11.84 KK508 pKa = 10.15 YY509 pKa = 10.4 ISINHH514 pKa = 5.27 YY515 pKa = 10.57 VEE517 pKa = 4.68 SCLTSGMRR525 pKa = 11.84 YY526 pKa = 9.82 AEE528 pKa = 4.32 YY529 pKa = 9.81 FYY531 pKa = 9.87 MQWDD535 pKa = 4.07 VVVPDD540 pKa = 4.44 AVTQEE545 pKa = 4.29 TEE547 pKa = 4.58 LINAKK552 pKa = 9.3 NQIFNFQKK560 pKa = 10.61 VVNTGNNDD568 pKa = 3.35 LPLGSLASNGFSIDD582 pKa = 3.15 SSSSQRR588 pKa = 11.84 IHH590 pKa = 6.07 QNEE593 pKa = 3.73 HH594 pKa = 5.1 AFYY597 pKa = 10.25 FKK599 pKa = 10.56 FIRR602 pKa = 11.84 ILPDD606 pKa = 3.41 LYY608 pKa = 11.0 YY609 pKa = 11.03 YY610 pKa = 11.14 GGIQRR615 pKa = 11.84 NKK617 pKa = 10.35 LFQKK621 pKa = 10.61 YY622 pKa = 9.91 LDD624 pKa = 3.78 YY625 pKa = 10.81 PLPQFSSLGNEE636 pKa = 3.88 EE637 pKa = 4.36 IYY639 pKa = 11.19 SNEE642 pKa = 3.69 VLFQFSNAKK651 pKa = 10.47 NIFGYY656 pKa = 9.81 NSRR659 pKa = 11.84 YY660 pKa = 9.77 SYY662 pKa = 11.76 SKK664 pKa = 10.76 FEE666 pKa = 4.37 PNKK669 pKa = 10.28 VSGDD673 pKa = 3.63 FCNSLSSQAFIRR685 pKa = 11.84 NLNEE689 pKa = 3.62 EE690 pKa = 4.57 DD691 pKa = 3.47 SDD693 pKa = 4.27 LSLRR697 pKa = 11.84 CSYY700 pKa = 10.61 EE701 pKa = 3.77 FNLVVNDD708 pKa = 3.6 KK709 pKa = 11.06 RR710 pKa = 11.84 PFAVISPNYY719 pKa = 9.88 DD720 pKa = 3.0 SYY722 pKa = 11.56 ICHH725 pKa = 6.8 SLIKK729 pKa = 10.16 FNNWRR734 pKa = 11.84 KK735 pKa = 9.69 LPVYY739 pKa = 8.98 GLPGIHH745 pKa = 6.98 TII747 pKa = 4.58
Molecular weight: 86.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.805
IPC2_protein 4.851
IPC_protein 4.825
Toseland 4.698
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.685
Rodwell 4.698
Grimsley 4.609
Solomon 4.8
Lehninger 4.762
Nozaki 4.914
DTASelect 5.118
Thurlkill 4.711
EMBOSS 4.724
Sillero 4.978
Patrickios 0.769
IPC_peptide 4.813
IPC2_peptide 4.965
IPC2.peptide.svr19 4.863
Protein with the highest isoelectric point:
>tr|A0A4P8W619|A0A4P8W619_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_414 OX=2585447 PE=4 SV=1
MM1 pKa = 7.56 NYY3 pKa = 10.07 KK4 pKa = 10.17 KK5 pKa = 10.27 QFKK8 pKa = 10.25 NFRR11 pKa = 11.84 NNDD14 pKa = 2.9 FFINYY19 pKa = 8.15 DD20 pKa = 3.25 KK21 pKa = 10.96 SYY23 pKa = 11.25 KK24 pKa = 9.98 NLPTIQGDD32 pKa = 3.86 VNRR35 pKa = 11.84 GISNLIQRR43 pKa = 11.84 YY44 pKa = 8.67 NRR46 pKa = 11.84 GLPLPNDD53 pKa = 3.48 AVQVSFGYY61 pKa = 8.65 TLEE64 pKa = 4.35 ASSPFTPDD72 pKa = 3.21 KK73 pKa = 11.32 LEE75 pKa = 4.07 LAIKK79 pKa = 10.25 SKK81 pKa = 10.67 QFNNTLSDD89 pKa = 3.29 VKK91 pKa = 10.13 TQLEE95 pKa = 4.25 KK96 pKa = 10.77 QIKK99 pKa = 8.12 TKK101 pKa = 9.63 EE102 pKa = 4.0 LEE104 pKa = 3.92 KK105 pKa = 10.74 RR106 pKa = 11.84 SAVEE110 pKa = 3.48 NTGNIEE116 pKa = 4.09 TSTTSS121 pKa = 2.78
Molecular weight: 13.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.983
IPC2_protein 8.858
IPC_protein 8.799
Toseland 9.604
ProMoST 9.268
Dawson 9.823
Bjellqvist 9.458
Wikipedia 9.97
Rodwell 10.262
Grimsley 9.897
Solomon 9.867
Lehninger 9.838
Nozaki 9.56
DTASelect 9.458
Thurlkill 9.663
EMBOSS 9.999
Sillero 9.721
Patrickios 7.629
IPC_peptide 9.867
IPC2_peptide 7.863
IPC2.peptide.svr19 8.015
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1574
121
747
314.8
36.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.066 ± 1.042
1.271 ± 0.416
5.972 ± 0.631
4.956 ± 0.451
6.099 ± 0.907
3.177 ± 0.282
1.398 ± 0.368
7.243 ± 0.554
7.306 ± 1.575
9.466 ± 0.327
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.334 ± 0.336
9.657 ± 0.659
4.257 ± 0.704
5.083 ± 0.554
3.367 ± 0.235
7.687 ± 0.791
5.337 ± 0.504
4.701 ± 0.753
0.508 ± 0.129
7.116 ± 0.844
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here