Cardiobacteriales bacterium ML27

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cardiobacteriales; Ostreibacteriaceae; Ostreibacterium

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1905 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7EVF2|A0A6N7EVF2_9GAMM ABC transporter permease subunit OS=Cardiobacteriales bacterium ML27 OX=2654998 GN=GCU85_02345 PE=3 SV=1
MM1 pKa = 7.17YY2 pKa = 10.33RR3 pKa = 11.84SDD5 pKa = 4.2CQNQHH10 pKa = 5.9IMHH13 pKa = 6.39QFAGVFSSRR22 pKa = 11.84AKK24 pKa = 10.22PLVKK28 pKa = 10.26AICLLSGLACTMTIYY43 pKa = 10.5AQEE46 pKa = 4.08VTPVSVNSAGEE57 pKa = 3.85QAASGSYY64 pKa = 8.85WPAISADD71 pKa = 3.09GRR73 pKa = 11.84YY74 pKa = 8.94VAFVSDD80 pKa = 3.76ADD82 pKa = 3.86NLVVGDD88 pKa = 4.27TNGDD92 pKa = 3.42DD93 pKa = 5.5DD94 pKa = 5.15IFVHH98 pKa = 7.08DD99 pKa = 4.84RR100 pKa = 11.84EE101 pKa = 4.56TGEE104 pKa = 4.08TTRR107 pKa = 11.84VSVGSAGEE115 pKa = 3.85QANSQFSAPAISGDD129 pKa = 3.03GRR131 pKa = 11.84YY132 pKa = 8.93VAFISGADD140 pKa = 3.51NLVAGDD146 pKa = 4.12TNNLSDD152 pKa = 3.77IFVHH156 pKa = 7.08DD157 pKa = 4.4RR158 pKa = 11.84EE159 pKa = 4.44TGEE162 pKa = 4.14TNRR165 pKa = 11.84VSVGSAGTQATSGSYY180 pKa = 10.48SPAISADD187 pKa = 2.84GRR189 pKa = 11.84YY190 pKa = 8.94VAFEE194 pKa = 3.88SWADD198 pKa = 3.52NLVAGDD204 pKa = 4.14TNNEE208 pKa = 3.51SDD210 pKa = 3.58IFVHH214 pKa = 7.27DD215 pKa = 4.4RR216 pKa = 11.84EE217 pKa = 4.4TDD219 pKa = 2.91EE220 pKa = 4.2TTRR223 pKa = 11.84VSVSSAGTQANGGSEE238 pKa = 4.26SPAISADD245 pKa = 2.89GRR247 pKa = 11.84YY248 pKa = 8.88VAFEE252 pKa = 4.03SWVDD256 pKa = 3.4NLVAGDD262 pKa = 3.8TNNRR266 pKa = 11.84TDD268 pKa = 3.1IFVHH272 pKa = 6.4DD273 pKa = 4.57RR274 pKa = 11.84EE275 pKa = 4.56TGEE278 pKa = 4.08TTRR281 pKa = 11.84VSVGSAGEE289 pKa = 3.85QANSQFSAPAISGDD303 pKa = 3.03GRR305 pKa = 11.84YY306 pKa = 8.95VAFVSSADD314 pKa = 3.58NLVAGDD320 pKa = 3.78TTNNNWDD327 pKa = 3.45VFVRR331 pKa = 11.84NLEE334 pKa = 3.87TGEE337 pKa = 4.16IKK339 pKa = 10.65RR340 pKa = 11.84ITADD344 pKa = 2.84IPYY347 pKa = 9.81EE348 pKa = 4.06EE349 pKa = 5.25EE350 pKa = 3.65ISSAVYY356 pKa = 10.47YY357 pKa = 10.18RR358 pKa = 11.84VSLSADD364 pKa = 2.87GRR366 pKa = 11.84YY367 pKa = 8.96IAYY370 pKa = 10.04SPEE373 pKa = 3.73YY374 pKa = 9.27QACDD378 pKa = 3.61FLGCGPSFYY387 pKa = 10.7GIYY390 pKa = 9.92IYY392 pKa = 10.6DD393 pKa = 3.63QEE395 pKa = 4.49TGQTEE400 pKa = 4.52VIAALLDD407 pKa = 3.79EE408 pKa = 4.86NNEE411 pKa = 4.52FGNSWPYY418 pKa = 10.5NGSGFPSLSADD429 pKa = 3.39GQTVAFGSSATNLVPNDD446 pKa = 3.48TNEE449 pKa = 3.84AFDD452 pKa = 3.74IFVAEE457 pKa = 4.23LGEE460 pKa = 4.42TISNISGTWYY470 pKa = 10.3DD471 pKa = 3.88RR472 pKa = 11.84NQSGG476 pKa = 3.81

Molecular weight: 50.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7EVQ6|A0A6N7EVQ6_9GAMM Uncharacterized protein OS=Cardiobacteriales bacterium ML27 OX=2654998 GN=GCU85_00220 PE=4 SV=1
MM1 pKa = 6.65KK2 pKa = 10.14QKK4 pKa = 9.7RR5 pKa = 11.84TFQPSNIKK13 pKa = 10.11RR14 pKa = 11.84KK15 pKa = 9.04RR16 pKa = 11.84SHH18 pKa = 6.18GFRR21 pKa = 11.84ARR23 pKa = 11.84MATAGGRR30 pKa = 11.84AVINARR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.67GRR41 pKa = 11.84KK42 pKa = 8.91RR43 pKa = 11.84LSAA46 pKa = 3.96

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1905

0

1905

628582

29

3124

330.0

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.821 ± 0.071

1.005 ± 0.02

5.669 ± 0.049

5.17 ± 0.056

4.007 ± 0.04

6.677 ± 0.055

2.315 ± 0.031

6.979 ± 0.047

4.852 ± 0.057

10.018 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.403 ± 0.026

4.378 ± 0.044

4.146 ± 0.036

4.882 ± 0.052

4.58 ± 0.034

6.133 ± 0.045

6.165 ± 0.047

6.578 ± 0.047

1.107 ± 0.021

3.115 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski