Leuconostoc carnosum (strain JB16)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Leuconostoc; Leuconostoc carnosum

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0D540|K0D540_LEUCJ Demethylmenaquinone methyltransferase OS=Leuconostoc carnosum (strain JB16) OX=1229758 GN=menG PE=3 SV=1
MM1 pKa = 7.52TNNEE5 pKa = 4.11LEE7 pKa = 4.28ILASEE12 pKa = 5.33LPILDD17 pKa = 4.6DD18 pKa = 4.01KK19 pKa = 11.55QDD21 pKa = 3.35YY22 pKa = 10.81KK23 pKa = 11.38YY24 pKa = 10.58FEE26 pKa = 5.07EE27 pKa = 4.21IAGIGSYY34 pKa = 9.13YY35 pKa = 9.65HH36 pKa = 6.41VAWQKK41 pKa = 11.55KK42 pKa = 8.05LDD44 pKa = 4.07GYY46 pKa = 9.95IALYY50 pKa = 8.13GTTPIEE56 pKa = 4.54LKK58 pKa = 11.06NIDD61 pKa = 3.41TSNDD65 pKa = 3.04VFEE68 pKa = 5.71DD69 pKa = 4.12EE70 pKa = 4.62EE71 pKa = 4.54VV72 pKa = 3.07

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0DCA0|K0DCA0_LEUCJ Integral membrane protein OS=Leuconostoc carnosum (strain JB16) OX=1229758 GN=C270_08071 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.34KK9 pKa = 7.47RR10 pKa = 11.84HH11 pKa = 5.71RR12 pKa = 11.84EE13 pKa = 3.7RR14 pKa = 11.84VHH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.23VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1690

0

1690

513563

28

2824

303.9

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.12 ± 0.064

0.186 ± 0.009

5.892 ± 0.06

5.411 ± 0.064

4.35 ± 0.043

6.525 ± 0.057

2.133 ± 0.028

7.666 ± 0.062

6.09 ± 0.057

9.715 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.03

5.049 ± 0.046

3.396 ± 0.031

4.756 ± 0.049

3.931 ± 0.048

6.082 ± 0.05

6.377 ± 0.048

7.234 ± 0.043

1.033 ± 0.023

3.381 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski