Clostridium sp. CAG:575

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1338 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6I0W2|R6I0W2_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:575 OX=1262823 GN=BN717_00849 PE=3 SV=1
MM1 pKa = 7.58SEE3 pKa = 4.39SNLNEE8 pKa = 4.07QIEE11 pKa = 4.33NLLKK15 pKa = 10.43EE16 pKa = 4.08MKK18 pKa = 9.85EE19 pKa = 3.59IKK21 pKa = 10.04EE22 pKa = 4.13NQKK25 pKa = 10.6EE26 pKa = 4.23MTEE29 pKa = 4.37KK30 pKa = 9.58MSKK33 pKa = 7.05MQQVIDD39 pKa = 4.77HH40 pKa = 6.92IEE42 pKa = 3.7SDD44 pKa = 3.31IYY46 pKa = 11.31ADD48 pKa = 3.37EE49 pKa = 5.05GFDD52 pKa = 4.16FEE54 pKa = 5.45IVCPYY59 pKa = 10.15CEE61 pKa = 4.25HH62 pKa = 6.85EE63 pKa = 4.45FVIDD67 pKa = 3.45ADD69 pKa = 4.05EE70 pKa = 4.54NKK72 pKa = 10.94DD73 pKa = 3.66EE74 pKa = 4.8IEE76 pKa = 4.5CPEE79 pKa = 4.29CKK81 pKa = 10.45NIIEE85 pKa = 4.95LDD87 pKa = 3.12WSGDD91 pKa = 3.52IEE93 pKa = 4.21EE94 pKa = 5.64HH95 pKa = 6.94EE96 pKa = 5.1DD97 pKa = 3.62GCSGHH102 pKa = 6.86CCGCAGCDD110 pKa = 4.82DD111 pKa = 4.98EE112 pKa = 7.14DD113 pKa = 5.89NEE115 pKa = 5.81DD116 pKa = 4.68DD117 pKa = 3.59MM118 pKa = 7.93

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6HDL0|R6HDL0_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:575 OX=1262823 GN=BN717_01130 PE=4 SV=1
MM1 pKa = 6.9VGKK4 pKa = 10.19RR5 pKa = 11.84RR6 pKa = 11.84NLLNYY11 pKa = 9.3LAKK14 pKa = 10.31KK15 pKa = 10.17DD16 pKa = 3.57VQRR19 pKa = 11.84YY20 pKa = 8.35RR21 pKa = 11.84DD22 pKa = 3.51IVEE25 pKa = 3.92KK26 pKa = 10.99LGLRR30 pKa = 11.84KK31 pKa = 9.66

Molecular weight: 3.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1338

0

1338

387873

30

4217

289.9

33.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.967 ± 0.059

1.082 ± 0.027

5.571 ± 0.08

8.351 ± 0.087

3.859 ± 0.062

5.655 ± 0.06

1.146 ± 0.025

10.483 ± 0.096

10.131 ± 0.073

8.183 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.038

7.216 ± 0.092

2.438 ± 0.036

2.987 ± 0.038

3.12 ± 0.047

5.625 ± 0.056

5.766 ± 0.088

5.886 ± 0.05

0.656 ± 0.02

4.468 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski