Celeribacter halophilus
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3717 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I3T4X5|A0A1I3T4X5_9RHOB Uncharacterized protein OS=Celeribacter halophilus OX=576117 GN=SAMN04488138_107192 PE=4 SV=1
MM1 pKa = 7.06 KK2 pKa = 9.29 QTYY5 pKa = 10.12 LSAALILGAGMAQAGEE21 pKa = 4.29 YY22 pKa = 10.21 CLLDD26 pKa = 3.45 GTEE29 pKa = 4.38 MFTCTFNGGTKK40 pKa = 10.11 AVEE43 pKa = 3.96 LCDD46 pKa = 4.89 AVWEE50 pKa = 4.8 DD51 pKa = 4.6 GDD53 pKa = 3.81 MASYY57 pKa = 11.24 GFFKK61 pKa = 11.16 SNGDD65 pKa = 3.48 VEE67 pKa = 5.49 KK68 pKa = 10.7 EE69 pKa = 3.64 ILQEE73 pKa = 4.01 KK74 pKa = 8.42 VTMLATPWNGMGNYY88 pKa = 9.34 VSEE91 pKa = 4.54 SVRR94 pKa = 11.84 FDD96 pKa = 3.44 ADD98 pKa = 2.81 EE99 pKa = 4.53 SYY101 pKa = 11.03 SYY103 pKa = 10.3 EE104 pKa = 3.86 VWSGGEE110 pKa = 3.7 RR111 pKa = 11.84 AEE113 pKa = 4.51 GAEE116 pKa = 4.17 LDD118 pKa = 3.86 GGINVIKK125 pKa = 10.36 DD126 pKa = 3.5 NEE128 pKa = 4.41 VIATLTCDD136 pKa = 3.32 TGSVTSDD143 pKa = 3.14 LGTLIDD149 pKa = 5.02 MIDD152 pKa = 3.87 LAQVSPP158 pKa = 4.1
Molecular weight: 16.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.795
IPC_protein 3.757
Toseland 3.554
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.605
EMBOSS 3.656
Sillero 3.872
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A1I3RRV4|A0A1I3RRV4_9RHOB Membrane protein insertase YidC OS=Celeribacter halophilus OX=576117 GN=yidC PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 AGRR28 pKa = 11.84 KK29 pKa = 8.46 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3717
0
3717
1141706
18
1825
307.2
33.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.434 ± 0.054
0.9 ± 0.012
5.949 ± 0.039
6.373 ± 0.037
3.878 ± 0.027
8.309 ± 0.042
2.065 ± 0.018
5.42 ± 0.03
3.807 ± 0.033
10.036 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.886 ± 0.02
2.693 ± 0.022
4.884 ± 0.03
3.138 ± 0.021
6.109 ± 0.041
5.526 ± 0.028
5.635 ± 0.028
7.295 ± 0.03
1.314 ± 0.017
2.347 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here