Amino acid dipepetide frequency for Pseudomonas sp. HAR-UPW-AIA-41

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.129AlaAla: 13.129 ± 0.15	1.327AlaCys: 1.327 ± 0.04	5.925AlaAsp: 5.925 ± 0.078	8.206AlaGlu: 8.206 ± 0.084	3.618AlaPhe: 3.618 ± 0.068	9.382AlaGly: 9.382 ± 0.115	2.163AlaHis: 2.163 ± 0.045	5.024AlaIle: 5.024 ± 0.081	3.691AlaLys: 3.691 ± 0.073	14.375AlaLeu: 14.375 ± 0.143	2.786AlaMet: 2.786 ± 0.052	2.853AlaAsn: 2.853 ± 0.052	4.696AlaPro: 4.696 ± 0.087	5.331AlaGln: 5.331 ± 0.079	7.434AlaArg: 7.434 ± 0.095	6.232AlaSer: 6.232 ± 0.078	4.531AlaThr: 4.531 ± 0.08	7.399AlaVal: 7.399 ± 0.084	1.651AlaTrp: 1.651 ± 0.041	2.495AlaTyr: 2.495 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	1.12CysAla: 1.12 ± 0.034	0.146CysCys: 0.146 ± 0.012	0.559CysAsp: 0.559 ± 0.023	0.579CysGlu: 0.579 ± 0.02	0.355CysPhe: 0.355 ± 0.019	0.987CysGly: 0.987 ± 0.028	0.29CysHis: 0.29 ± 0.017	0.418CysIle: 0.418 ± 0.022	0.282CysLys: 0.282 ± 0.017	1.197CysLeu: 1.197 ± 0.038	0.221CysMet: 0.221 ± 0.013	0.281CysAsn: 0.281 ± 0.016	0.573CysPro: 0.573 ± 0.022	0.502CysGln: 0.502 ± 0.024	0.692CysArg: 0.692 ± 0.028	0.676CysSer: 0.676 ± 0.025	0.419CysThr: 0.419 ± 0.02	0.703CysVal: 0.703 ± 0.027	0.164CysTrp: 0.164 ± 0.012	0.254CysTyr: 0.254 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.491AspAla: 5.491 ± 0.079	0.572AspCys: 0.572 ± 0.024	2.676AspAsp: 2.676 ± 0.064	3.647AspGlu: 3.647 ± 0.065	2.076AspPhe: 2.076 ± 0.048	4.364AspGly: 4.364 ± 0.094	1.046AspHis: 1.046 ± 0.031	2.485AspIle: 2.485 ± 0.045	1.888AspLys: 1.888 ± 0.052	6.024AspLeu: 6.024 ± 0.071	1.192AspMet: 1.192 ± 0.034	1.576AspAsn: 1.576 ± 0.037	2.62AspPro: 2.62 ± 0.047	2.146AspGln: 2.146 ± 0.052	2.821AspArg: 2.821 ± 0.052	3.061AspSer: 3.061 ± 0.055	2.143AspThr: 2.143 ± 0.05	3.296AspVal: 3.296 ± 0.062	0.983AspTrp: 0.983 ± 0.027	1.71AspTyr: 1.71 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	7.081GluAla: 7.081 ± 0.084	0.456GluCys: 0.456 ± 0.021	2.601GluAsp: 2.601 ± 0.048	3.53GluGlu: 3.53 ± 0.065	1.9GluPhe: 1.9 ± 0.039	4.151GluGly: 4.151 ± 0.069	1.659GluHis: 1.659 ± 0.045	2.757GluIle: 2.757 ± 0.06	2.106GluLys: 2.106 ± 0.056	7.986GluLeu: 7.986 ± 0.096	1.349GluMet: 1.349 ± 0.04	1.454GluAsn: 1.454 ± 0.041	2.548GluPro: 2.548 ± 0.055	4.627GluGln: 4.627 ± 0.079	5.079GluArg: 5.079 ± 0.074	2.764GluSer: 2.764 ± 0.051	2.398GluThr: 2.398 ± 0.045	4.544GluVal: 4.544 ± 0.066	0.757GluTrp: 0.757 ± 0.025	1.229GluTyr: 1.229 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.137PheAla: 4.137 ± 0.073	0.47PheCys: 0.47 ± 0.024	2.276PheAsp: 2.276 ± 0.041	2.013PheGlu: 2.013 ± 0.047	1.389PhePhe: 1.389 ± 0.044	3.057PheGly: 3.057 ± 0.064	0.713PheHis: 0.713 ± 0.025	1.751PheIle: 1.751 ± 0.041	1.188PheLys: 1.188 ± 0.029	3.225PheLeu: 3.225 ± 0.067	0.77PheMet: 0.77 ± 0.024	1.307PheAsn: 1.307 ± 0.037	1.41PhePro: 1.41 ± 0.037	1.244PheGln: 1.244 ± 0.034	1.883PheArg: 1.883 ± 0.042	2.507PheSer: 2.507 ± 0.047	1.835PheThr: 1.835 ± 0.045	2.378PheVal: 2.378 ± 0.054	0.547PheTrp: 0.547 ± 0.023	1.029PheTyr: 1.029 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	7.038GlyAla: 7.038 ± 0.096	1.006GlyCys: 1.006 ± 0.031	3.904GlyAsp: 3.904 ± 0.068	5.232GlyGlu: 5.232 ± 0.076	3.133GlyPhe: 3.133 ± 0.061	5.947GlyGly: 5.947 ± 0.111	1.889GlyHis: 1.889 ± 0.043	4.057GlyIle: 4.057 ± 0.069	3.296GlyLys: 3.296 ± 0.06	9.558GlyLeu: 9.558 ± 0.101	2.134GlyMet: 2.134 ± 0.044	2.366GlyAsn: 2.366 ± 0.063	2.478GlyPro: 2.478 ± 0.058	3.92GlyGln: 3.92 ± 0.064	4.976GlyArg: 4.976 ± 0.076	4.608GlySer: 4.608 ± 0.098	3.39GlyThr: 3.39 ± 0.07	5.788GlyVal: 5.788 ± 0.092	1.291GlyTrp: 1.291 ± 0.037	2.414GlyTyr: 2.414 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.236HisAla: 2.236 ± 0.048	0.342HisCys: 0.342 ± 0.018	1.143HisAsp: 1.143 ± 0.031	1.276HisGlu: 1.276 ± 0.036	0.979HisPhe: 0.979 ± 0.033	1.965HisGly: 1.965 ± 0.049	0.574HisHis: 0.574 ± 0.026	0.93HisIle: 0.93 ± 0.034	0.639HisLys: 0.639 ± 0.025	2.691HisLeu: 2.691 ± 0.054	0.533HisMet: 0.533 ± 0.023	0.633HisAsn: 0.633 ± 0.022	1.392HisPro: 1.392 ± 0.035	1.025HisGln: 1.025 ± 0.031	1.284HisArg: 1.284 ± 0.034	1.294HisSer: 1.294 ± 0.034	0.905HisThr: 0.905 ± 0.029	1.283HisVal: 1.283 ± 0.034	0.468HisTrp: 0.468 ± 0.021	0.751HisTyr: 0.751 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.465IleAla: 5.465 ± 0.078	0.458IleCys: 0.458 ± 0.023	3.077IleAsp: 3.077 ± 0.058	3.328IleGlu: 3.328 ± 0.064	1.321IlePhe: 1.321 ± 0.036	4.223IleGly: 4.223 ± 0.072	0.928IleHis: 0.928 ± 0.028	1.914IleIle: 1.914 ± 0.047	1.602IleLys: 1.602 ± 0.044	4.114IleLeu: 4.114 ± 0.062	0.766IleMet: 0.766 ± 0.024	1.658IleAsn: 1.658 ± 0.039	2.113IlePro: 2.113 ± 0.047	1.601IleGln: 1.601 ± 0.036	2.952IleArg: 2.952 ± 0.057	2.964IleSer: 2.964 ± 0.061	2.384IleThr: 2.384 ± 0.05	2.823IleVal: 2.823 ± 0.055	0.462IleTrp: 0.462 ± 0.024	1.044IleTyr: 1.044 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.047LysAla: 4.047 ± 0.067	0.209LysCys: 0.209 ± 0.016	1.687LysAsp: 1.687 ± 0.049	1.586LysGlu: 1.586 ± 0.047	0.823LysPhe: 0.823 ± 0.028	2.509LysGly: 2.509 ± 0.056	0.705LysHis: 0.705 ± 0.028	1.454LysIle: 1.454 ± 0.038	1.302LysLys: 1.302 ± 0.052	3.809LysLeu: 3.809 ± 0.07	0.651LysMet: 0.651 ± 0.027	0.951LysAsn: 0.951 ± 0.031	2.099LysPro: 2.099 ± 0.046	1.596LysGln: 1.596 ± 0.038	2.372LysArg: 2.372 ± 0.047	1.728LysSer: 1.728 ± 0.04	1.699LysThr: 1.699 ± 0.046	2.639LysVal: 2.639 ± 0.052	0.338LysTrp: 0.338 ± 0.014	0.656LysTyr: 0.656 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	15.408LeuAla: 15.408 ± 0.164	1.355LeuCys: 1.355 ± 0.034	6.82LeuAsp: 6.82 ± 0.096	6.912LeuGlu: 6.912 ± 0.1	4.24LeuPhe: 4.24 ± 0.062	9.452LeuGly: 9.452 ± 0.104	2.589LeuHis: 2.589 ± 0.048	5.511LeuIle: 5.511 ± 0.07	4.466LeuLys: 4.466 ± 0.073	15.935LeuLeu: 15.935 ± 0.216	2.447LeuMet: 2.447 ± 0.045	3.659LeuAsn: 3.659 ± 0.056	6.99LeuPro: 6.99 ± 0.091	5.953LeuGln: 5.953 ± 0.086	8.157LeuArg: 8.157 ± 0.102	7.527LeuSer: 7.527 ± 0.097	5.805LeuThr: 5.805 ± 0.088	7.817LeuVal: 7.817 ± 0.095	1.612LeuTrp: 1.612 ± 0.041	2.709LeuTyr: 2.709 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.687MetAla: 2.687 ± 0.049	0.161MetCys: 0.161 ± 0.012	1.029MetAsp: 1.029 ± 0.033	0.936MetGlu: 0.936 ± 0.032	0.585MetPhe: 0.585 ± 0.022	1.573MetGly: 1.573 ± 0.035	0.516MetHis: 0.516 ± 0.023	0.962MetIle: 0.962 ± 0.032	0.813MetLys: 0.813 ± 0.029	2.754MetLeu: 2.754 ± 0.052	0.438MetMet: 0.438 ± 0.02	0.77MetAsn: 0.77 ± 0.028	1.325MetPro: 1.325 ± 0.039	1.13MetGln: 1.13 ± 0.031	1.475MetArg: 1.475 ± 0.042	1.649MetSer: 1.649 ± 0.042	1.255MetThr: 1.255 ± 0.033	1.424MetVal: 1.424 ± 0.042	0.16MetTrp: 0.16 ± 0.012	0.349MetTyr: 0.349 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.052AsnAla: 3.052 ± 0.06	0.301AsnCys: 0.301 ± 0.017	1.459AsnAsp: 1.459 ± 0.041	1.453AsnGlu: 1.453 ± 0.041	1.039AsnPhe: 1.039 ± 0.034	2.355AsnGly: 2.355 ± 0.067	0.57AsnHis: 0.57 ± 0.02	1.334AsnIle: 1.334 ± 0.041	0.893AsnLys: 0.893 ± 0.028	3.514AsnLeu: 3.514 ± 0.056	0.564AsnMet: 0.564 ± 0.023	0.896AsnAsn: 0.896 ± 0.038	1.958AsnPro: 1.958 ± 0.044	1.389AsnGln: 1.389 ± 0.037	1.877AsnArg: 1.877 ± 0.038	1.631AsnSer: 1.631 ± 0.04	1.316AsnThr: 1.316 ± 0.041	1.779AsnVal: 1.779 ± 0.05	0.452AsnTrp: 0.452 ± 0.021	0.774AsnTyr: 0.774 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	6.189ProAla: 6.189 ± 0.107	0.377ProCys: 0.377 ± 0.018	2.587ProAsp: 2.587 ± 0.054	3.412ProGlu: 3.412 ± 0.061	1.725ProPhe: 1.725 ± 0.034	3.946ProGly: 3.946 ± 0.052	1.05ProHis: 1.05 ± 0.033	1.816ProIle: 1.816 ± 0.045	1.42ProLys: 1.42 ± 0.039	6.106ProLeu: 6.106 ± 0.085	1.095ProMet: 1.095 ± 0.034	1.314ProAsn: 1.314 ± 0.038	1.915ProPro: 1.915 ± 0.048	2.531ProGln: 2.531 ± 0.05	2.713ProArg: 2.713 ± 0.062	2.45ProSer: 2.45 ± 0.053	1.999ProThr: 1.999 ± 0.04	3.778ProVal: 3.778 ± 0.062	0.802ProTrp: 0.802 ± 0.028	1.201ProTyr: 1.201 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	6.67GlnAla: 6.67 ± 0.102	0.383GlnCys: 0.383 ± 0.022	1.887GlnAsp: 1.887 ± 0.043	2.233GlnGlu: 2.233 ± 0.049	1.56GlnPhe: 1.56 ± 0.038	3.736GlnGly: 3.736 ± 0.066	1.367GlnHis: 1.367 ± 0.039	2.1GlnIle: 2.1 ± 0.045	1.244GlnLys: 1.244 ± 0.043	6.877GlnLeu: 6.877 ± 0.116	1.109GlnMet: 1.109 ± 0.036	1.118GlnAsn: 1.118 ± 0.031	2.811GlnPro: 2.811 ± 0.053	3.633GlnGln: 3.633 ± 0.088	4.184GlnArg: 4.184 ± 0.078	2.443GlnSer: 2.443 ± 0.051	1.898GlnThr: 1.898 ± 0.051	3.843GlnVal: 3.843 ± 0.059	0.746GlnTrp: 0.746 ± 0.032	1.043GlnTyr: 1.043 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	6.225ArgAla: 6.225 ± 0.086	0.669ArgCys: 0.669 ± 0.026	3.545ArgAsp: 3.545 ± 0.059	4.766ArgGlu: 4.766 ± 0.068	2.761ArgPhe: 2.761 ± 0.057	4.179ArgGly: 4.179 ± 0.066	1.791ArgHis: 1.791 ± 0.043	3.221ArgIle: 3.221 ± 0.052	1.908ArgLys: 1.908 ± 0.05	9.23ArgLeu: 9.23 ± 0.112	1.532ArgMet: 1.532 ± 0.039	1.806ArgAsn: 1.806 ± 0.046	2.786ArgPro: 2.786 ± 0.053	4.101ArgGln: 4.101 ± 0.078	4.501ArgArg: 4.501 ± 0.076	3.38ArgSer: 3.38 ± 0.062	2.469ArgThr: 2.469 ± 0.048	4.415ArgVal: 4.415 ± 0.068	1.13ArgTrp: 1.13 ± 0.036	2.106ArgTyr: 2.106 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.095SerAla: 6.095 ± 0.079	0.547SerCys: 0.547 ± 0.023	2.808SerAsp: 2.808 ± 0.055	3.405SerGlu: 3.405 ± 0.061	2.14SerPhe: 2.14 ± 0.048	5.101SerGly: 5.101 ± 0.089	1.27SerHis: 1.27 ± 0.031	2.498SerIle: 2.498 ± 0.052	1.694SerLys: 1.694 ± 0.045	7.598SerLeu: 7.598 ± 0.1	1.308SerMet: 1.308 ± 0.036	1.716SerAsn: 1.716 ± 0.05	2.568SerPro: 2.568 ± 0.043	2.725SerGln: 2.725 ± 0.053	3.585SerArg: 3.585 ± 0.059	3.454SerSer: 3.454 ± 0.075	2.618SerThr: 2.618 ± 0.058	3.672SerVal: 3.672 ± 0.064	0.816SerTrp: 0.816 ± 0.025	1.466SerTyr: 1.466 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	4.845ThrAla: 4.845 ± 0.074	0.444ThrCys: 0.444 ± 0.023	2.18ThrAsp: 2.18 ± 0.059	2.24ThrGlu: 2.24 ± 0.049	1.605ThrPhe: 1.605 ± 0.042	3.831ThrGly: 3.831 ± 0.075	0.911ThrHis: 0.911 ± 0.025	1.779ThrIle: 1.779 ± 0.052	1.0ThrLys: 1.0 ± 0.035	6.433ThrLeu: 6.433 ± 0.091	0.67ThrMet: 0.67 ± 0.027	1.061ThrAsn: 1.061 ± 0.04	2.925ThrPro: 2.925 ± 0.057	1.897ThrGln: 1.897 ± 0.039	2.853ThrArg: 2.853 ± 0.055	2.398ThrSer: 2.398 ± 0.061	2.099ThrThr: 2.099 ± 0.051	3.091ThrVal: 3.091 ± 0.064	0.604ThrTrp: 0.604 ± 0.024	1.139ThrTyr: 1.139 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	7.649ValAla: 7.649 ± 0.093	0.724ValCys: 0.724 ± 0.026	3.768ValAsp: 3.768 ± 0.065	4.499ValGlu: 4.499 ± 0.066	2.401ValPhe: 2.401 ± 0.055	4.765ValGly: 4.765 ± 0.069	1.351ValHis: 1.351 ± 0.038	3.622ValIle: 3.622 ± 0.061	2.298ValLys: 2.298 ± 0.058	8.251ValLeu: 8.251 ± 0.091	1.656ValMet: 1.656 ± 0.038	2.063ValAsn: 2.063 ± 0.057	3.198ValPro: 3.198 ± 0.061	2.87ValGln: 2.87 ± 0.047	4.435ValArg: 4.435 ± 0.064	4.005ValSer: 4.005 ± 0.064	3.218ValThr: 3.218 ± 0.075	5.071ValVal: 5.071 ± 0.075	0.825ValTrp: 0.825 ± 0.029	1.544ValTyr: 1.544 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.14TrpAla: 1.14 ± 0.034	0.162TrpCys: 0.162 ± 0.013	0.632TrpAsp: 0.632 ± 0.024	0.549TrpGlu: 0.549 ± 0.025	0.522TrpPhe: 0.522 ± 0.026	0.892TrpGly: 0.892 ± 0.029	0.396TrpHis: 0.396 ± 0.022	0.596TrpIle: 0.596 ± 0.023	0.396TrpLys: 0.396 ± 0.018	2.503TrpLeu: 2.503 ± 0.057	0.354TrpMet: 0.354 ± 0.017	0.414TrpAsn: 0.414 ± 0.019	0.709TrpPro: 0.709 ± 0.026	1.197TrpGln: 1.197 ± 0.034	1.164TrpArg: 1.164 ± 0.037	0.771TrpSer: 0.771 ± 0.026	0.547TrpThr: 0.547 ± 0.024	0.938TrpVal: 0.938 ± 0.031	0.241TrpTrp: 0.241 ± 0.017	0.375TrpTyr: 0.375 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.542TyrAla: 2.542 ± 0.055	0.31TyrCys: 0.31 ± 0.015	1.257TyrAsp: 1.257 ± 0.038	1.172TyrGlu: 1.172 ± 0.039	0.956TyrPhe: 0.956 ± 0.03	2.01TyrGly: 2.01 ± 0.05	0.566TyrHis: 0.566 ± 0.023	0.97TyrIle: 0.97 ± 0.035	0.734TyrLys: 0.734 ± 0.028	3.182TyrLeu: 3.182 ± 0.057	0.414TyrMet: 0.414 ± 0.022	0.712TyrAsn: 0.712 ± 0.03	1.31TyrPro: 1.31 ± 0.033	1.467TyrGln: 1.467 ± 0.041	2.086TyrArg: 2.086 ± 0.044	1.545TyrSer: 1.545 ± 0.038	1.077TyrThr: 1.077 ± 0.033	1.532TyrVal: 1.532 ± 0.043	0.447TyrTrp: 0.447 ± 0.019	0.705TyrTyr: 0.705 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3419 proteins (1101474 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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