Rhodobium orientis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhodobiaceae; Rhodobium

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4696 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327JHB0|A0A327JHB0_9RHIZ Alanine acetyltransferase OS=Rhodobium orientis OX=34017 GN=CH339_18020 PE=3 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84SDD4 pKa = 3.91SPDD7 pKa = 2.95ISSGIGGTLIAYY19 pKa = 9.77LDD21 pKa = 3.48RR22 pKa = 11.84RR23 pKa = 11.84DD24 pKa = 4.66NYY26 pKa = 8.54TCCGATNLSDD36 pKa = 4.31RR37 pKa = 11.84DD38 pKa = 3.35IGTGVASDD46 pKa = 3.73GFYY49 pKa = 10.75AIANAGPAAVILDD62 pKa = 3.75VGSAKK67 pKa = 10.42ALSGIAIYY75 pKa = 10.29GGYY78 pKa = 10.36GNRR81 pKa = 11.84DD82 pKa = 3.3DD83 pKa = 5.26GAYY86 pKa = 9.01TLKK89 pKa = 10.93DD90 pKa = 3.29GNGTVLGSWTVSNTSGGTNDD110 pKa = 5.49GIDD113 pKa = 3.98SYY115 pKa = 10.78WLSFKK120 pKa = 10.46TVVTTDD126 pKa = 3.04KK127 pKa = 11.24LVIDD131 pKa = 4.49TEE133 pKa = 4.11NAEE136 pKa = 4.24NGTSSFRR143 pKa = 11.84EE144 pKa = 3.71IEE146 pKa = 4.31VFSANSPEE154 pKa = 3.58IRR156 pKa = 11.84IAVIGDD162 pKa = 4.11SYY164 pKa = 11.26TEE166 pKa = 4.43GYY168 pKa = 10.58GDD170 pKa = 3.37NCDD173 pKa = 4.21INVCSQLPTPKK184 pKa = 10.23SEE186 pKa = 3.77FTTSYY191 pKa = 9.75EE192 pKa = 4.12AAPVMPGG199 pKa = 2.8

Molecular weight: 20.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327JQC6|A0A327JQC6_9RHIZ ABC transporter substrate-binding protein OS=Rhodobium orientis OX=34017 GN=CH339_09440 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.84LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATNGGRR28 pKa = 11.84KK29 pKa = 9.15VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4696

0

4696

1496865

30

6852

318.8

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.494 ± 0.05

0.878 ± 0.011

6.164 ± 0.034

6.126 ± 0.031

3.861 ± 0.021

8.63 ± 0.032

2.005 ± 0.017

5.3 ± 0.029

3.382 ± 0.027

9.78 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.017

2.508 ± 0.021

5.099 ± 0.031

2.554 ± 0.019

7.094 ± 0.035

5.128 ± 0.023

5.392 ± 0.021

7.649 ± 0.027

1.263 ± 0.014

2.233 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski