Sorex araneus polyomavirus 1
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A223PYK3|A0A223PYK3_9POLY Minor capsid protein VP2 OS=Sorex araneus polyomavirus 1 OX=2560769 GN=VP3 PE=3 SV=1
MM1 pKa = 7.37 APKK4 pKa = 10.47 RR5 pKa = 11.84 KK6 pKa = 6.14 TTCSSKK12 pKa = 9.45 KK13 pKa = 8.44 TCPQPSSVPKK23 pKa = 10.51 LIIKK27 pKa = 10.25 GGIEE31 pKa = 3.93 VLDD34 pKa = 4.01 VKK36 pKa = 10.35 TGDD39 pKa = 4.0 DD40 pKa = 4.31 SITQIEE46 pKa = 4.54 AFLNPRR52 pKa = 11.84 MGVNDD57 pKa = 4.35 EE58 pKa = 4.21 TNTWYY63 pKa = 10.59 GFSEE67 pKa = 4.38 QVTVATARR75 pKa = 11.84 EE76 pKa = 3.94 TDD78 pKa = 3.64 RR79 pKa = 11.84 PPKK82 pKa = 10.11 EE83 pKa = 3.54 QMPYY87 pKa = 9.5 YY88 pKa = 9.62 SCARR92 pKa = 11.84 IPLPLLNEE100 pKa = 4.49 DD101 pKa = 3.74 MTCNTLLMWEE111 pKa = 4.19 AVSVKK116 pKa = 10.22 TEE118 pKa = 4.18 VIGSNTLMNVHH129 pKa = 7.52 DD130 pKa = 4.39 YY131 pKa = 7.47 MTRR134 pKa = 11.84 TDD136 pKa = 3.72 NGVGHH141 pKa = 6.66 PVVGSTYY148 pKa = 11.08 HH149 pKa = 5.27 MFAVGGEE156 pKa = 3.99 PLDD159 pKa = 3.7 LQGIQQSHH167 pKa = 6.0 LVQYY171 pKa = 10.09 PEE173 pKa = 3.97 GLIVPKK179 pKa = 10.65 SVTDD183 pKa = 3.39 VTAKK187 pKa = 10.0 IQCLDD192 pKa = 3.64 PSAKK196 pKa = 10.4 AKK198 pKa = 10.26 LDD200 pKa = 3.43 KK201 pKa = 10.76 DD202 pKa = 3.46 GKK204 pKa = 10.83 YY205 pKa = 10.26 PIEE208 pKa = 4.22 TWSPDD213 pKa = 3.05 PSRR216 pKa = 11.84 NEE218 pKa = 3.42 NTRR221 pKa = 11.84 YY222 pKa = 9.46 FGNYY226 pKa = 9.01 YY227 pKa = 10.64 GGLTTPPVLTFTNTVTTILLDD248 pKa = 3.88 EE249 pKa = 5.0 NGVGPLCKK257 pKa = 10.08 GDD259 pKa = 4.6 GLFLSCCDD267 pKa = 3.3 VMGWFTAGSGTHH279 pKa = 4.67 QRR281 pKa = 11.84 FRR283 pKa = 11.84 GLPRR287 pKa = 11.84 YY288 pKa = 10.0 FNVQLRR294 pKa = 11.84 KK295 pKa = 9.21 RR296 pKa = 11.84 AVRR299 pKa = 11.84 NPYY302 pKa = 9.14 PVSALLTSLFTNMMPRR318 pKa = 11.84 MSGQPMIGNKK328 pKa = 9.41 SQVEE332 pKa = 4.1 EE333 pKa = 4.0 VRR335 pKa = 11.84 VYY337 pKa = 10.85 EE338 pKa = 4.78 GLEE341 pKa = 3.9 QLPGDD346 pKa = 4.14 PDD348 pKa = 3.59 MEE350 pKa = 4.08 RR351 pKa = 11.84 HH352 pKa = 5.77 IDD354 pKa = 3.57 EE355 pKa = 5.26 FGQEE359 pKa = 4.0 ITPVPP364 pKa = 3.72
Molecular weight: 40.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.363
IPC2_protein 5.461
IPC_protein 5.436
Toseland 5.563
ProMoST 5.664
Dawson 5.55
Bjellqvist 5.614
Wikipedia 5.474
Rodwell 5.499
Grimsley 5.588
Solomon 5.55
Lehninger 5.525
Nozaki 5.728
DTASelect 5.906
Thurlkill 5.741
EMBOSS 5.703
Sillero 5.817
Patrickios 3.948
IPC_peptide 5.563
IPC2_peptide 5.817
IPC2.peptide.svr19 5.83
Protein with the highest isoelectric point:
>tr|A0A223PYK8|A0A223PYK8_9POLY Large t antigen OS=Sorex araneus polyomavirus 1 OX=2560769 GN=LTAg PE=4 SV=1
MM1 pKa = 8.27 DD2 pKa = 6.0 SILTFAEE9 pKa = 4.02 RR10 pKa = 11.84 QLLISLLKK18 pKa = 10.52 ISGDD22 pKa = 3.44 TFGNVPAMARR32 pKa = 11.84 AYY34 pKa = 9.7 KK35 pKa = 10.24 LAAKK39 pKa = 10.03 RR40 pKa = 11.84 LHH42 pKa = 6.69 PDD44 pKa = 2.56 KK45 pKa = 11.14 GGNEE49 pKa = 3.97 AEE51 pKa = 4.11 MKK53 pKa = 10.37 KK54 pKa = 10.82 LNEE57 pKa = 3.92 LWNKK61 pKa = 9.79 FKK63 pKa = 11.22 DD64 pKa = 3.97 GIYY67 pKa = 10.41 NLRR70 pKa = 11.84 EE71 pKa = 4.3 VKK73 pKa = 10.27 PSLHH77 pKa = 6.26 PVVTCTVLGARR88 pKa = 11.84 NIFNLITNSSQCMRR102 pKa = 11.84 NLLRR106 pKa = 11.84 YY107 pKa = 8.49 CRR109 pKa = 11.84 CFCCILFQQHH119 pKa = 5.59 RR120 pKa = 11.84 QLKK123 pKa = 6.75 ITYY126 pKa = 9.65 RR127 pKa = 11.84 RR128 pKa = 11.84 RR129 pKa = 11.84 CNVWGQCYY137 pKa = 10.14 CFLCYY142 pKa = 9.12 YY143 pKa = 9.47 TWFGVNCSIGAFTEE157 pKa = 3.93 WLILLKK163 pKa = 10.81 HH164 pKa = 6.36 LDD166 pKa = 3.17 WRR168 pKa = 11.84 LLKK171 pKa = 10.39 ISSAEE176 pKa = 3.79 LDD178 pKa = 3.84 VLGKK182 pKa = 10.4
Molecular weight: 21.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.837
IPC2_protein 8.609
IPC_protein 8.507
Toseland 8.902
ProMoST 9.107
Dawson 9.355
Bjellqvist 9.414
Wikipedia 9.458
Rodwell 9.502
Grimsley 9.18
Solomon 9.443
Lehninger 9.428
Nozaki 9.633
DTASelect 9.209
Thurlkill 9.326
EMBOSS 9.516
Sillero 9.545
Patrickios 4.457
IPC_peptide 9.428
IPC2_peptide 8.58
IPC2.peptide.svr19 7.934
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1764
182
708
352.8
39.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.519 ± 1.133
2.324 ± 0.739
5.102 ± 0.784
5.612 ± 0.355
4.025 ± 0.709
7.313 ± 0.796
2.494 ± 0.23
5.612 ± 0.376
6.066 ± 1.406
9.694 ± 1.137
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.685 ± 0.54
4.592 ± 0.826
5.612 ± 0.741
4.252 ± 0.661
5.215 ± 0.772
5.896 ± 0.594
5.839 ± 0.811
5.782 ± 0.643
1.587 ± 0.581
2.778 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here