Blastococcus sp. AZ43

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3926 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9NDR5|A0A4Y9NDR5_9ACTN Uncharacterized protein OS=Blastococcus sp. AZ43 OX=2559606 GN=E4P37_10640 PE=4 SV=1
MM1 pKa = 7.91SYY3 pKa = 10.46PPQAPHH9 pKa = 5.45QQYY12 pKa = 10.61GPPPSYY18 pKa = 10.94APPPKK23 pKa = 9.99QGAGLAIASMVLGIIALLLSWIPIINNVAAVIALVGLGLGIPALLRR69 pKa = 11.84ARR71 pKa = 11.84RR72 pKa = 11.84GTHH75 pKa = 5.9SGTGMAITGLVTSVIAIVLVILTQLLFVEE104 pKa = 5.66AIEE107 pKa = 4.19EE108 pKa = 4.27VEE110 pKa = 3.98KK111 pKa = 11.08SINEE115 pKa = 4.44GIEE118 pKa = 3.94QSEE121 pKa = 5.06LDD123 pKa = 3.93PADD126 pKa = 3.61PTAEE130 pKa = 4.0AEE132 pKa = 4.42EE133 pKa = 4.45PVAAPEE139 pKa = 4.05VVPLGAPAQVGDD151 pKa = 3.81YY152 pKa = 10.29EE153 pKa = 4.84VIVDD157 pKa = 4.97AVEE160 pKa = 4.18LDD162 pKa = 3.73ANATVAAANDD172 pKa = 4.05FNEE175 pKa = 4.7PPAGQYY181 pKa = 10.8VITQLTVTYY190 pKa = 9.17TGTDD194 pKa = 2.81EE195 pKa = 6.11GMPGWDD201 pKa = 3.21LTAVFHH207 pKa = 6.31GTDD210 pKa = 2.9NRR212 pKa = 11.84QYY214 pKa = 11.65SDD216 pKa = 3.89TEE218 pKa = 4.2CSAVLPDD225 pKa = 4.85DD226 pKa = 5.7AMDD229 pKa = 4.94APTLNPGGVDD239 pKa = 3.28TFQFCMDD246 pKa = 4.37VAPTAIPGGQLTIEE260 pKa = 4.29PTVSFGSDD268 pKa = 2.82EE269 pKa = 4.1RR270 pKa = 11.84VAFAIQQ276 pKa = 2.86

Molecular weight: 28.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9N748|A0A4Y9N748_9ACTN ATP-binding protein OS=Blastococcus sp. AZ43 OX=2559606 GN=E4P37_14825 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3926

0

3926

1219815

19

1870

310.7

33.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.186 ± 0.065

0.677 ± 0.01

6.26 ± 0.029

5.779 ± 0.037

2.613 ± 0.021

9.596 ± 0.035

1.967 ± 0.019

3.004 ± 0.029

1.492 ± 0.024

10.719 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.684 ± 0.015

1.472 ± 0.019

6.125 ± 0.031

2.663 ± 0.02

8.126 ± 0.04

4.804 ± 0.026

5.865 ± 0.027

9.72 ± 0.036

1.482 ± 0.016

1.767 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski