Capybara microvirus Cap1_SP_228
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4E5|A0A4P8W4E5_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_228 OX=2585415 PE=4 SV=1
MM1 pKa = 7.36 TRR3 pKa = 11.84 LQGFNDD9 pKa = 3.43 SGVEE13 pKa = 3.9 MRR15 pKa = 11.84 GSYY18 pKa = 11.19 VMVEE22 pKa = 3.92 TDD24 pKa = 3.16 YY25 pKa = 11.23 STDD28 pKa = 3.67 CSCDD32 pKa = 2.62 GMTRR36 pKa = 11.84 QEE38 pKa = 4.75 FADD41 pKa = 3.65 EE42 pKa = 4.01 CDD44 pKa = 3.38 INVLMSRR51 pKa = 11.84 YY52 pKa = 8.63 EE53 pKa = 3.71 KK54 pKa = 10.89 SGVLNHH60 pKa = 6.11 YY61 pKa = 9.94 NGAEE65 pKa = 3.72 AAYY68 pKa = 10.37 LDD70 pKa = 4.66 LSDD73 pKa = 5.03 VPDD76 pKa = 4.06 LATAFDD82 pKa = 3.88 IFEE85 pKa = 4.45 KK86 pKa = 11.05 ANTAFMSLPAAVRR99 pKa = 11.84 RR100 pKa = 11.84 EE101 pKa = 4.0 FEE103 pKa = 3.91 NDD105 pKa = 2.96 PVRR108 pKa = 11.84 FADD111 pKa = 5.61 FAADD115 pKa = 3.59 PANVDD120 pKa = 5.03 KK121 pKa = 10.88 MRR123 pKa = 11.84 EE124 pKa = 3.96 WKK126 pKa = 10.1 LAAPAPVEE134 pKa = 4.01 PGPTKK139 pKa = 10.79 VEE141 pKa = 3.79 IVNPPEE147 pKa = 4.57 SGSSGATKK155 pKa = 10.51
Molecular weight: 17.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.378
IPC2_protein 4.304
IPC_protein 4.24
Toseland 4.05
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.101
Rodwell 4.062
Grimsley 3.961
Solomon 4.19
Lehninger 4.151
Nozaki 4.304
DTASelect 4.495
Thurlkill 4.075
EMBOSS 4.113
Sillero 4.355
Patrickios 3.719
IPC_peptide 4.202
IPC2_peptide 4.329
IPC2.peptide.svr19 4.282
Protein with the highest isoelectric point:
>tr|A0A4P8W868|A0A4P8W868_9VIRU Nonstructural protein OS=Capybara microvirus Cap1_SP_228 OX=2585415 PE=4 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.23 GAYY5 pKa = 9.78 APFALSSEE13 pKa = 4.01 IVFIAFCTAAMSLSRR28 pKa = 11.84 ISFMSGFRR36 pKa = 11.84 SFVFARR42 pKa = 11.84 FRR44 pKa = 11.84 LAISFSSAAVKK55 pKa = 10.09 IALSISIFSGILVSLCAGCTRR76 pKa = 11.84 FLSIVQGVCQCALL89 pKa = 3.31
Molecular weight: 9.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.151
IPC2_protein 8.741
IPC_protein 8.887
Toseland 9.092
ProMoST 9.18
Dawson 9.458
Bjellqvist 9.633
Wikipedia 9.502
Rodwell 9.545
Grimsley 8.916
Solomon 9.736
Lehninger 9.736
Nozaki 9.97
DTASelect 9.399
Thurlkill 9.487
EMBOSS 9.663
Sillero 9.765
Patrickios 7.395
IPC_peptide 9.721
IPC2_peptide 9.428
IPC2.peptide.svr19 7.971
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1409
81
528
234.8
25.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.51 ± 1.024
1.419 ± 0.649
5.394 ± 0.758
4.471 ± 0.896
5.678 ± 0.76
7.736 ± 0.47
1.632 ± 0.305
3.762 ± 0.658
4.542 ± 1.205
7.097 ± 0.645
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.839 ± 0.291
4.613 ± 0.862
5.039 ± 0.737
4.542 ± 0.986
6.175 ± 0.805
8.375 ± 0.928
6.175 ± 0.905
6.175 ± 0.643
0.923 ± 0.165
3.903 ± 0.498
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here