Bacillus sp. 1NLA3E

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4384 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N0AXL4|N0AXL4_9BACI RNA polymerase sigma factor OS=Bacillus sp. 1NLA3E OX=666686 GN=B1NLA3E_15005 PE=3 SV=1
MM1 pKa = 7.68AKK3 pKa = 9.2FTIVDD8 pKa = 3.73KK9 pKa = 8.19EE10 pKa = 4.36TCIACGACGAAAPDD24 pKa = 3.33IYY26 pKa = 10.99DD27 pKa = 3.84YY28 pKa = 11.45DD29 pKa = 4.3DD30 pKa = 3.75EE31 pKa = 7.04GIAFVTLDD39 pKa = 3.76DD40 pKa = 3.74NQGIVEE46 pKa = 4.27IPDD49 pKa = 3.53VLVDD53 pKa = 4.09DD54 pKa = 4.64MMDD57 pKa = 3.37AFEE60 pKa = 4.9GCPTDD65 pKa = 3.71SIKK68 pKa = 10.87VAEE71 pKa = 4.38EE72 pKa = 4.18SFDD75 pKa = 3.7GNATKK80 pKa = 10.67FEE82 pKa = 4.22

Molecular weight: 8.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N0AYP5|N0AYP5_9BACI Uncharacterized protein OS=Bacillus sp. 1NLA3E OX=666686 GN=B1NLA3E_08845 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4384

0

4384

1276292

37

2748

291.1

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.672 ± 0.036

0.813 ± 0.014

4.929 ± 0.028

7.046 ± 0.047

4.725 ± 0.029

6.96 ± 0.037

2.001 ± 0.016

8.271 ± 0.041

7.255 ± 0.035

9.82 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.02

4.7 ± 0.029

3.614 ± 0.024

3.693 ± 0.024

3.942 ± 0.025

6.119 ± 0.028

5.359 ± 0.025

6.961 ± 0.033

0.999 ± 0.015

3.404 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski