Enterobacter phage PG7
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 294 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W6ATF9|W6ATF9_9CAUD Uncharacterized protein OS=Enterobacter phage PG7 OX=1455074 GN=PG7_157 PE=4 SV=1
MM1 pKa = 7.83 KK2 pKa = 10.21 EE3 pKa = 4.03 ILWNSAFGQTIPKK16 pKa = 9.32 GYY18 pKa = 10.52 AVSCVSWEE26 pKa = 3.98 NDD28 pKa = 2.63 GDD30 pKa = 4.11 DD31 pKa = 3.68 YY32 pKa = 11.48 QEE34 pKa = 3.93 NVIYY38 pKa = 10.26 AVNARR43 pKa = 11.84 HH44 pKa = 5.22 VSGWCAVAKK53 pKa = 10.39 CFASDD58 pKa = 3.37 NSDD61 pKa = 3.59 PNGMGNEE68 pKa = 4.59 DD69 pKa = 3.59 YY70 pKa = 11.35 SGDD73 pKa = 3.64 AFFEE77 pKa = 4.57 YY78 pKa = 10.1 CVEE81 pKa = 4.11 NEE83 pKa = 4.09 EE84 pKa = 4.27 ITPEE88 pKa = 3.97 FCSEE92 pKa = 3.88 VLGFVIPEE100 pKa = 3.75 GDD102 pKa = 3.63 YY103 pKa = 11.51 TEE105 pKa = 5.89 DD106 pKa = 3.34 EE107 pKa = 4.04 FDD109 pKa = 4.15 ARR111 pKa = 11.84 LEE113 pKa = 4.04 DD114 pKa = 5.01 FYY116 pKa = 11.53 QVQYY120 pKa = 11.64 KK121 pKa = 10.57 VMDD124 pKa = 5.01 KK125 pKa = 10.12 IQEE128 pKa = 4.48 LIGTPVGYY136 pKa = 10.31 DD137 pKa = 2.79 IDD139 pKa = 3.97 FMRR142 pKa = 11.84 VAEE145 pKa = 4.29 TFKK148 pKa = 10.95 LFYY151 pKa = 10.15 IEE153 pKa = 4.15 EE154 pKa = 5.11 DD155 pKa = 3.11 IVIPKK160 pKa = 10.09 APEE163 pKa = 4.7 AITVVSII170 pKa = 4.23
Molecular weight: 19.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.803
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.63
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.088
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.948
Patrickios 0.477
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|W6ATD3|W6ATD3_9CAUD Uncharacterized protein OS=Enterobacter phage PG7 OX=1455074 GN=PG7_122 PE=4 SV=1
MM1 pKa = 7.34 SRR3 pKa = 11.84 TIRR6 pKa = 11.84 RR7 pKa = 11.84 KK8 pKa = 9.93 GFNKK12 pKa = 8.76 RR13 pKa = 11.84 TSACRR18 pKa = 11.84 FQQYY22 pKa = 10.8 NNEE25 pKa = 4.18 FYY27 pKa = 10.7 HH28 pKa = 6.09 LCKK31 pKa = 9.95 DD32 pKa = 3.91 TYY34 pKa = 10.54 RR35 pKa = 11.84 CMIIRR40 pKa = 11.84 WRR42 pKa = 11.84 HH43 pKa = 3.63 NN44 pKa = 3.02
Molecular weight: 5.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.882
IPC_protein 10.804
Toseland 10.672
ProMoST 10.496
Dawson 10.804
Bjellqvist 10.584
Wikipedia 11.067
Rodwell 10.935
Grimsley 10.877
Solomon 10.95
Lehninger 10.906
Nozaki 10.701
DTASelect 10.57
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.76
Patrickios 10.716
IPC_peptide 10.95
IPC2_peptide 9.94
IPC2.peptide.svr19 8.207
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
294
0
294
53483
21
1264
181.9
20.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.055 ± 0.195
1.069 ± 0.06
6.215 ± 0.109
7.331 ± 0.173
4.373 ± 0.116
6.391 ± 0.195
1.859 ± 0.084
6.903 ± 0.117
7.29 ± 0.167
7.419 ± 0.12
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.911 ± 0.088
5.248 ± 0.147
3.631 ± 0.094
3.395 ± 0.085
4.643 ± 0.102
6.062 ± 0.125
5.787 ± 0.187
6.853 ± 0.132
1.531 ± 0.062
4.033 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here