Staphylococcus phage B236

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus B236

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3XC69|A0A0E3XC69_9CAUD Head morphogenesis domain protein OS=Staphylococcus phage B236 OX=1636205 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 4.48MMNNRR7 pKa = 11.84EE8 pKa = 4.09QIEE11 pKa = 3.98QSVISASAYY20 pKa = 10.04NGNDD24 pKa = 3.29TEE26 pKa = 4.52GLLKK30 pKa = 10.49EE31 pKa = 4.44IEE33 pKa = 4.24DD34 pKa = 4.28VYY36 pKa = 11.57KK37 pKa = 10.64KK38 pKa = 10.72AQAFDD43 pKa = 4.61EE44 pKa = 4.37ILEE47 pKa = 4.31GMTNAIQHH55 pKa = 5.29SVKK58 pKa = 10.58EE59 pKa = 4.41GIEE62 pKa = 3.75LDD64 pKa = 3.71EE65 pKa = 4.98AVGIMTGQVVYY76 pKa = 10.25KK77 pKa = 10.67YY78 pKa = 10.65EE79 pKa = 3.96EE80 pKa = 3.91EE81 pKa = 4.19QEE83 pKa = 4.16NEE85 pKa = 4.03EE86 pKa = 4.29FF87 pKa = 4.47

Molecular weight: 9.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3T8L8|A0A0E3T8L8_9CAUD Uncharacterized protein OS=Staphylococcus phage B236 OX=1636205 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84FKK4 pKa = 10.55WEE6 pKa = 4.08QKK8 pKa = 10.07HH9 pKa = 5.15IDD11 pKa = 3.88YY12 pKa = 10.93VKK14 pKa = 10.31SIYY17 pKa = 9.71KK18 pKa = 10.05GRR20 pKa = 11.84YY21 pKa = 7.9LKK23 pKa = 10.51DD24 pKa = 3.19VRR26 pKa = 11.84DD27 pKa = 3.62MFNNKK32 pKa = 9.48FGTNISKK39 pKa = 10.41NAINAKK45 pKa = 7.76MNRR48 pKa = 11.84LGVKK52 pKa = 9.99SGVSPYY58 pKa = 10.85GSRR61 pKa = 11.84QAHH64 pKa = 5.1GHH66 pKa = 4.39LHH68 pKa = 6.1KK69 pKa = 10.7PIGSKK74 pKa = 10.7KK75 pKa = 9.76LDD77 pKa = 3.1QHH79 pKa = 6.66GYY81 pKa = 10.25VLIKK85 pKa = 10.49VDD87 pKa = 4.03NKK89 pKa = 10.56TNNQRR94 pKa = 11.84NNWKK98 pKa = 10.18LYY100 pKa = 9.05HH101 pKa = 6.23HH102 pKa = 6.24YY103 pKa = 10.32VWEE106 pKa = 3.99KK107 pKa = 10.27HH108 pKa = 5.6YY109 pKa = 11.69GEE111 pKa = 5.29IPPKK115 pKa = 10.16HH116 pKa = 6.06AVIFLNGNKK125 pKa = 9.53KK126 pKa = 10.3DD127 pKa = 3.8FRR129 pKa = 11.84IEE131 pKa = 3.66NLAMITYY138 pKa = 7.8STLQMMINNNLFYY151 pKa = 11.12EE152 pKa = 4.29NAEE155 pKa = 4.08LTKK158 pKa = 10.57TGINIAKK165 pKa = 9.77LLKK168 pKa = 9.24KK169 pKa = 7.41QTRR172 pKa = 11.84RR173 pKa = 11.84SVKK176 pKa = 9.92KK177 pKa = 9.36MKK179 pKa = 10.23IKK181 pKa = 10.46EE182 pKa = 4.11KK183 pKa = 10.43EE184 pKa = 3.79

Molecular weight: 21.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13567

53

961

202.5

23.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.786 ± 0.337

0.405 ± 0.06

6.383 ± 0.254

7.732 ± 0.48

4.165 ± 0.208

5.845 ± 0.306

1.835 ± 0.161

7.43 ± 0.231

9.206 ± 0.402

7.371 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.159

6.486 ± 0.215

2.816 ± 0.166

4.054 ± 0.234

4.113 ± 0.242

5.83 ± 0.293

6.258 ± 0.252

6.044 ± 0.25

1.209 ± 0.152

4.422 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski