Comamonas kerstersii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Comamonas

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2662 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W7YZN4|A0A0W7YZN4_9BURK Acyl-CoA dehydrogenase OS=Comamonas kerstersii OX=225992 GN=AS359_03625 PE=3 SV=1
MM1 pKa = 7.26SAVAEE6 pKa = 4.25NTTTEE11 pKa = 4.03MPEE14 pKa = 4.03PIIFTDD20 pKa = 3.47SAAAKK25 pKa = 10.02VADD28 pKa = 5.45LIAEE32 pKa = 4.31EE33 pKa = 4.82GNPDD37 pKa = 2.99LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.94GFTFDD59 pKa = 4.69EE60 pKa = 4.93IVNEE64 pKa = 5.02DD65 pKa = 4.04DD66 pKa = 3.2TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.57QYY85 pKa = 11.72LLGAEE90 pKa = 3.98IDD92 pKa = 4.02YY93 pKa = 11.34KK94 pKa = 11.22EE95 pKa = 4.67DD96 pKa = 3.23LQGAQFVIKK105 pKa = 10.4NPNATTTCGCGSSFSVV121 pKa = 3.54

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W7YTD2|A0A0W7YTD2_9BURK Succinate--CoA ligase [ADP-forming] subunit alpha OS=Comamonas kerstersii OX=225992 GN=sucD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.3QPSKK9 pKa = 7.96TKK11 pKa = 10.33RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.85GFLVRR21 pKa = 11.84MKK23 pKa = 9.4TKK25 pKa = 10.4GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2662

0

2662

856518

37

2528

321.8

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.103 ± 0.061

0.985 ± 0.017

4.946 ± 0.039

5.484 ± 0.048

3.417 ± 0.033

7.739 ± 0.043

2.421 ± 0.026

4.67 ± 0.039

3.868 ± 0.044

10.64 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.026

2.854 ± 0.026

4.878 ± 0.035

5.252 ± 0.046

5.908 ± 0.04

5.462 ± 0.034

5.107 ± 0.028

7.556 ± 0.041

1.552 ± 0.026

2.31 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski