Monkeypox virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 174 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q98VL5|Q98VL5_MONPV Bcl-2-like protein IFN-beta inhibitor OS=Monkeypox virus OX=10244 GN=D15L PE=3 SV=1
MM1 pKa = 6.7TQLPILLLLISLVYY15 pKa = 9.16ATPSPQTSKK24 pKa = 11.19KK25 pKa = 10.09IGDD28 pKa = 4.35DD29 pKa = 3.33ATISCSRR36 pKa = 11.84NNTNYY41 pKa = 9.79YY42 pKa = 10.37VVMSAWYY49 pKa = 9.51KK50 pKa = 9.87EE51 pKa = 4.05PNSIILLAAKK61 pKa = 10.26SDD63 pKa = 3.41VLYY66 pKa = 10.72FDD68 pKa = 5.73NYY70 pKa = 9.77TKK72 pKa = 11.04DD73 pKa = 3.41KK74 pKa = 10.63ISYY77 pKa = 9.41DD78 pKa = 3.49SPYY81 pKa = 10.83DD82 pKa = 3.76DD83 pKa = 5.42LVTTITIKK91 pKa = 10.92SLTAGDD97 pKa = 3.78AGTYY101 pKa = 9.34ICAFFMTSTTNDD113 pKa = 3.24TDD115 pKa = 3.65KK116 pKa = 11.31VDD118 pKa = 3.6YY119 pKa = 10.56EE120 pKa = 4.4EE121 pKa = 4.7YY122 pKa = 10.52SIEE125 pKa = 4.85LIVNTDD131 pKa = 3.33SEE133 pKa = 4.71STIDD137 pKa = 3.89IILSGSTPEE146 pKa = 4.54TISEE150 pKa = 4.11KK151 pKa = 10.87PEE153 pKa = 5.47DD154 pKa = 4.15IDD156 pKa = 4.57NSNCSSVFEE165 pKa = 4.37IATPEE170 pKa = 4.75PITDD174 pKa = 3.41NVEE177 pKa = 3.87DD178 pKa = 3.72HH179 pKa = 7.12TDD181 pKa = 3.32TVTYY185 pKa = 9.79TSDD188 pKa = 4.27SINTVNASSGEE199 pKa = 4.28STTDD203 pKa = 3.18EE204 pKa = 4.32TPEE207 pKa = 4.89PITDD211 pKa = 3.52KK212 pKa = 11.57EE213 pKa = 4.01EE214 pKa = 4.05DD215 pKa = 3.46HH216 pKa = 6.73TVTDD220 pKa = 3.97TVSYY224 pKa = 7.19TTVSTSSGIVTTKK237 pKa = 9.79STTDD241 pKa = 3.36DD242 pKa = 3.45ADD244 pKa = 5.14LYY246 pKa = 10.71DD247 pKa = 4.24TYY249 pKa = 11.51NDD251 pKa = 3.68NDD253 pKa = 4.34TVPPTTVGGSTTSISNYY270 pKa = 6.38KK271 pKa = 9.3TKK273 pKa = 10.95DD274 pKa = 3.25FVEE277 pKa = 4.2IFGITTLIILSAVAIFCITYY297 pKa = 7.41YY298 pKa = 10.07ICNKK302 pKa = 9.68HH303 pKa = 4.71PRR305 pKa = 11.84KK306 pKa = 10.27YY307 pKa = 9.24KK308 pKa = 10.11TEE310 pKa = 3.97NKK312 pKa = 9.17VV313 pKa = 2.9

Molecular weight: 34.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5IXN6|Q5IXN6_MONPV A30L OS=Monkeypox virus OX=10244 GN=A30L PE=3 SV=1
MM1 pKa = 7.68KK2 pKa = 10.3SLNRR6 pKa = 11.84QTVSRR11 pKa = 11.84FRR13 pKa = 11.84KK14 pKa = 9.63LSVPAAIMMLLSTIISGIGTFLHH37 pKa = 6.01YY38 pKa = 10.26RR39 pKa = 11.84EE40 pKa = 4.56EE41 pKa = 4.32LMPSACANGWIQYY54 pKa = 9.45DD55 pKa = 3.41KK56 pKa = 11.16HH57 pKa = 7.66CYY59 pKa = 10.14LDD61 pKa = 3.76TNIKK65 pKa = 9.77MSTDD69 pKa = 2.97NAVYY73 pKa = 9.87QCRR76 pKa = 11.84KK77 pKa = 9.5LRR79 pKa = 11.84ARR81 pKa = 11.84LPRR84 pKa = 11.84PDD86 pKa = 3.46TRR88 pKa = 11.84HH89 pKa = 5.59LRR91 pKa = 11.84VLFSIFYY98 pKa = 9.15KK99 pKa = 10.52DD100 pKa = 3.24YY101 pKa = 9.86WVSLKK106 pKa = 9.22KK107 pKa = 9.33TNDD110 pKa = 2.56KK111 pKa = 10.21WLDD114 pKa = 3.28INNDD118 pKa = 3.01KK119 pKa = 11.26DD120 pKa = 3.45IDD122 pKa = 3.75ISKK125 pKa = 8.87LTNFKK130 pKa = 10.39QLNSTTDD137 pKa = 3.03SEE139 pKa = 4.15ACYY142 pKa = 9.74IYY144 pKa = 10.89KK145 pKa = 10.22SGKK148 pKa = 8.99LVKK151 pKa = 8.76TVCKK155 pKa = 9.32STQSVLCVKK164 pKa = 10.35RR165 pKa = 11.84FYY167 pKa = 10.92KK168 pKa = 10.71

Molecular weight: 19.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

174

0

174

54645

53

1879

314.1

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.779 ± 0.12

2.042 ± 0.097

6.831 ± 0.137

5.442 ± 0.142

4.617 ± 0.135

4.041 ± 0.126

2.059 ± 0.085

9.49 ± 0.171

7.252 ± 0.128

8.718 ± 0.133

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.076

6.775 ± 0.12

3.393 ± 0.098

2.262 ± 0.08

4.29 ± 0.11

7.821 ± 0.136

6.238 ± 0.15

6.18 ± 0.11

0.712 ± 0.045

5.307 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski