Torque teno midi virus 8
Average proteome isoelectric point is 7.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VLY0|A7VLY0_9VIRU Capsid protein OS=Torque teno midi virus 8 OX=2065049 PE=3 SV=1
MM1 pKa = 7.16 QHH3 pKa = 6.75 LCSKK7 pKa = 10.96 DD8 pKa = 3.54 FIKK11 pKa = 10.37 PCKK14 pKa = 9.98 YY15 pKa = 10.54 NEE17 pKa = 3.98 EE18 pKa = 4.39 TKK20 pKa = 10.6 NQLWMSEE27 pKa = 4.22 LADD30 pKa = 4.65 SHH32 pKa = 7.26 DD33 pKa = 3.69 IWCSCDD39 pKa = 3.08 TPFAHH44 pKa = 7.44 LLASIFPPGHH54 pKa = 5.56 QDD56 pKa = 3.05 RR57 pKa = 11.84 GKK59 pKa = 8.84 TIQDD63 pKa = 3.04 ILEE66 pKa = 4.11 RR67 pKa = 11.84 DD68 pKa = 4.05 YY69 pKa = 11.58 KK70 pKa = 9.77 QLCHH74 pKa = 6.65 SGGPAEE80 pKa = 4.34 RR81 pKa = 11.84 SHH83 pKa = 7.38 GILTGTATGADD94 pKa = 3.25 IKK96 pKa = 11.27 EE97 pKa = 4.02 EE98 pKa = 4.58 DD99 pKa = 4.14 GLEE102 pKa = 4.15 GDD104 pKa = 4.02 AEE106 pKa = 4.39 EE107 pKa = 4.93 FLMAAAAAAAEE118 pKa = 4.39 EE119 pKa = 4.57 KK120 pKa = 10.61 EE121 pKa = 4.23 RR122 pKa = 4.7
Molecular weight: 13.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.739
IPC2_protein 4.8
IPC_protein 4.711
Toseland 4.609
ProMoST 4.838
Dawson 4.685
Bjellqvist 4.825
Wikipedia 4.546
Rodwell 4.584
Grimsley 4.533
Solomon 4.673
Lehninger 4.635
Nozaki 4.8
DTASelect 4.94
Thurlkill 4.609
EMBOSS 4.584
Sillero 4.863
Patrickios 3.719
IPC_peptide 4.685
IPC2_peptide 4.851
IPC2.peptide.svr19 4.802
Protein with the highest isoelectric point:
>tr|A7VLY0|A7VLY0_9VIRU Capsid protein OS=Torque teno midi virus 8 OX=2065049 PE=3 SV=1
MM1 pKa = 7.31 PFWWARR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 10.25 KK10 pKa = 9.8 PWYY13 pKa = 9.88 SNWNRR18 pKa = 11.84 YY19 pKa = 6.51 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 YY23 pKa = 8.8 KK24 pKa = 9.61 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 WPRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 IPYY38 pKa = 7.85 GRR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 KK48 pKa = 8.72 RR49 pKa = 11.84 KK50 pKa = 8.81 VRR52 pKa = 11.84 RR53 pKa = 11.84 KK54 pKa = 7.41 RR55 pKa = 11.84 QRR57 pKa = 11.84 IIVKK61 pKa = 8.67 QWQPEE66 pKa = 4.43 SIVKK70 pKa = 10.5 CEE72 pKa = 3.87 IKK74 pKa = 10.97 GLGCLVAGAQGRR86 pKa = 11.84 QPFCYY91 pKa = 9.55 TNSKK95 pKa = 10.36 YY96 pKa = 10.54 EE97 pKa = 3.96 YY98 pKa = 8.79 PQPKK102 pKa = 10.0 APGGGGFGAEE112 pKa = 4.52 LFTLQYY118 pKa = 11.06 LYY120 pKa = 10.94 DD121 pKa = 3.44 EE122 pKa = 4.42 WVARR126 pKa = 11.84 RR127 pKa = 11.84 NIWTKK132 pKa = 9.79 TNDD135 pKa = 3.36 YY136 pKa = 10.38 KK137 pKa = 11.52 DD138 pKa = 3.57 LVRR141 pKa = 11.84 FTGATFYY148 pKa = 10.72 LYY150 pKa = 10.58 KK151 pKa = 10.68 HH152 pKa = 6.27 PTTDD156 pKa = 5.35 FIFNYY161 pKa = 10.01 NNQPPFLLEE170 pKa = 3.59 KK171 pKa = 10.73 DD172 pKa = 4.62 FYY174 pKa = 11.67 NDD176 pKa = 3.27 LHH178 pKa = 6.57 PQMLLLAKK186 pKa = 9.04 HH187 pKa = 5.98 HH188 pKa = 6.28 RR189 pKa = 11.84 VIPSRR194 pKa = 11.84 QRR196 pKa = 11.84 KK197 pKa = 8.29 PLGKK201 pKa = 9.48 PYY203 pKa = 10.24 IKK205 pKa = 10.78 VKK207 pKa = 10.4 IKK209 pKa = 10.25 PPKK212 pKa = 9.87 QMLTEE217 pKa = 4.24 WFFQQDD223 pKa = 3.38 FATQGLFSIHH233 pKa = 6.24 AAAANMQWAYY243 pKa = 11.16 YY244 pKa = 10.72 NPDD247 pKa = 3.24 QQSRR251 pKa = 11.84 CLTLYY256 pKa = 10.61 SLNIKK261 pKa = 9.91 FFANTDD267 pKa = 2.75 WSATHH272 pKa = 7.08 TGTQMYY278 pKa = 9.25 FPYY281 pKa = 10.41 NAWPHH286 pKa = 5.26 TKK288 pKa = 10.37 GLTFWTIDD296 pKa = 3.27 SKK298 pKa = 11.74 GNKK301 pKa = 7.88 TPHH304 pKa = 6.43 SIKK307 pKa = 9.57 PQNYY311 pKa = 7.95 NQSVNYY317 pKa = 9.75 LQGFFSQYY325 pKa = 10.04 VLQAQKK331 pKa = 9.05 ITDD334 pKa = 3.69 ADD336 pKa = 4.11 DD337 pKa = 3.84 NVPAKK342 pKa = 10.65 YY343 pKa = 9.15 EE344 pKa = 4.04 QYY346 pKa = 10.2 SAAMSTLLIAAFRR359 pKa = 11.84 YY360 pKa = 9.99 NPEE363 pKa = 3.78 EE364 pKa = 3.93 DD365 pKa = 3.17 TGQGNVVWLTSTLSNTHH382 pKa = 5.59 WEE384 pKa = 4.36 RR385 pKa = 11.84 PQLSDD390 pKa = 3.93 LVIVGKK396 pKa = 8.49 PLWLSFYY403 pKa = 10.4 GFEE406 pKa = 5.12 SFIQKK411 pKa = 8.44 TKK413 pKa = 10.33 HH414 pKa = 5.85 DD415 pKa = 4.45 KK416 pKa = 10.8 GWLLSGFYY424 pKa = 10.21 VCKK427 pKa = 10.42 CPYY430 pKa = 10.56 LKK432 pKa = 9.78 TLSATTQTTFAFIDD446 pKa = 3.48 LDD448 pKa = 4.39 FIQGKK453 pKa = 9.26 LPYY456 pKa = 10.1 GEE458 pKa = 5.32 TITSQKK464 pKa = 9.55 KK465 pKa = 9.38 ALWYY469 pKa = 9.84 PNVEE473 pKa = 4.12 NQVNTVTNLVQCGPYY488 pKa = 8.73 IPKK491 pKa = 10.15 YY492 pKa = 10.36 AFRR495 pKa = 11.84 DD496 pKa = 3.5 EE497 pKa = 4.61 STWEE501 pKa = 3.72 LTYY504 pKa = 10.74 KK505 pKa = 10.69 YY506 pKa = 10.18 IFYY509 pKa = 9.72 FKK511 pKa = 10.09 WGGPYY516 pKa = 10.45 VSDD519 pKa = 4.94 QPVQNPKK526 pKa = 8.61 TQGKK530 pKa = 9.31 YY531 pKa = 9.39 PVPDD535 pKa = 3.63 TVSQAIQISNPLKK548 pKa = 8.7 QTCKK552 pKa = 10.81 AMLRR556 pKa = 11.84 DD557 pKa = 2.94 WDD559 pKa = 3.74 YY560 pKa = 11.54 RR561 pKa = 11.84 RR562 pKa = 11.84 GIITTTALKK571 pKa = 10.57 RR572 pKa = 11.84 MSEE575 pKa = 4.07 HH576 pKa = 6.36 LQTDD580 pKa = 3.47 SSVQSDD586 pKa = 3.96 DD587 pKa = 4.52 SEE589 pKa = 4.56 TPKK592 pKa = 10.74 KK593 pKa = 10.31 KK594 pKa = 10.5 EE595 pKa = 3.71 KK596 pKa = 7.79 TTAEE600 pKa = 3.46 IPYY603 pKa = 8.36 YY604 pKa = 8.52 QEE606 pKa = 3.77 KK607 pKa = 8.56 TEE609 pKa = 4.12 EE610 pKa = 4.1 MQSCLLSLFEE620 pKa = 5.35 EE621 pKa = 5.16 STCQEE626 pKa = 4.27 PEE628 pKa = 3.85 NLQQLILHH636 pKa = 5.81 QQQQQQKK643 pKa = 9.04 LKK645 pKa = 11.13 SNILKK650 pKa = 10.6 LLMDD654 pKa = 5.3 LKK656 pKa = 10.31 HH657 pKa = 5.45 QQRR660 pKa = 11.84 ILQHH664 pKa = 5.33 HH665 pKa = 6.26 TGIEE669 pKa = 3.91
Molecular weight: 79.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.855
IPC2_protein 9.282
IPC_protein 9.209
Toseland 9.75
ProMoST 9.545
Dawson 10.028
Bjellqvist 9.75
Wikipedia 10.218
Rodwell 10.335
Grimsley 10.116
Solomon 10.043
Lehninger 9.999
Nozaki 9.794
DTASelect 9.721
Thurlkill 9.853
EMBOSS 10.175
Sillero 9.955
Patrickios 5.041
IPC_peptide 10.028
IPC2_peptide 8.434
IPC2.peptide.svr19 8.224
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1306
122
669
326.5
37.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.662 ± 1.463
2.374 ± 0.598
4.594 ± 0.662
6.126 ± 1.358
4.058 ± 0.548
5.13 ± 0.426
3.292 ± 0.653
4.518 ± 0.325
8.729 ± 0.736
8.116 ± 0.309
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.761 ± 0.181
3.752 ± 0.545
5.896 ± 0.976
6.738 ± 1.181
5.666 ± 0.735
6.891 ± 1.628
6.968 ± 0.409
2.527 ± 0.798
2.144 ± 0.611
4.058 ± 1.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here