Aspergillus glaucus CBS 516.65
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11245 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L9VNY5|A0A1L9VNY5_ASPGL Uncharacterized protein OS=Aspergillus glaucus CBS 516.65 OX=1160497 GN=ASPGLDRAFT_1373063 PE=4 SV=1
MM1 pKa = 7.77 VYY3 pKa = 8.76 GTQNEE8 pKa = 4.13 DD9 pKa = 3.54 PEE11 pKa = 5.31 RR12 pKa = 11.84 GEE14 pKa = 3.96 YY15 pKa = 9.95 RR16 pKa = 11.84 SLYY19 pKa = 10.15 SDD21 pKa = 3.46 QDD23 pKa = 3.61 GDD25 pKa = 4.25 DD26 pKa = 4.14 TPSTTPLYY34 pKa = 8.29 THH36 pKa = 7.68 PSTAPNANNPEE47 pKa = 4.37 FMSKK51 pKa = 10.15 PNPRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 SWIQFQQNLRR67 pKa = 11.84 LPTSTEE73 pKa = 3.52 VSTIAKK79 pKa = 9.87 AVFGALGFIIGILVCTLAIITLIYY103 pKa = 10.53 GCTEE107 pKa = 3.75 VVNSIRR113 pKa = 11.84 HH114 pKa = 5.18 VDD116 pKa = 3.62 DD117 pKa = 4.31 PSPYY121 pKa = 9.93 RR122 pKa = 11.84 RR123 pKa = 11.84 LSDD126 pKa = 3.49 YY127 pKa = 10.41 QGYY130 pKa = 8.47 VAVEE134 pKa = 4.1 DD135 pKa = 5.84 DD136 pKa = 4.19 IDD138 pKa = 4.2 DD139 pKa = 4.9 PYY141 pKa = 10.53 PYY143 pKa = 9.62 PYY145 pKa = 10.32 SYY147 pKa = 10.91 PLTDD151 pKa = 3.47 GGGDD155 pKa = 3.21 VDD157 pKa = 4.22 TAPKK161 pKa = 11.09 AMTTKK166 pKa = 10.49 SGIEE170 pKa = 3.88 VACFGEE176 pKa = 4.71 AGTCEE181 pKa = 4.04 ASLSEE186 pKa = 5.05 LMRR189 pKa = 11.84 IPQATRR195 pKa = 11.84 VAVITDD201 pKa = 3.64 GEE203 pKa = 4.25 AWSDD207 pKa = 3.21 ITGEE211 pKa = 4.32 DD212 pKa = 3.63 LDD214 pKa = 5.54 GDD216 pKa = 3.89 VDD218 pKa = 4.03 IDD220 pKa = 4.0 GDD222 pKa = 3.98 GDD224 pKa = 3.73 GDD226 pKa = 3.57 EE227 pKa = 5.84 GEE229 pKa = 4.89 FPDD232 pKa = 5.72 EE233 pKa = 4.02 LACGNMWMEE242 pKa = 4.1 EE243 pKa = 4.1 DD244 pKa = 3.37 EE245 pKa = 4.58 GVVVDD250 pKa = 3.85 EE251 pKa = 4.83 LEE253 pKa = 4.28 YY254 pKa = 11.39 VSMLDD259 pKa = 3.73 RR260 pKa = 11.84 LWNGLFGGG268 pKa = 4.35
Molecular weight: 29.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.656
EMBOSS 3.745
Sillero 3.948
Patrickios 1.252
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A1L9VJB7|A0A1L9VJB7_ASPGL Carrier domain-containing protein OS=Aspergillus glaucus CBS 516.65 OX=1160497 GN=ASPGLDRAFT_26190 PE=4 SV=1
MM1 pKa = 7.52 FSRR4 pKa = 11.84 RR5 pKa = 11.84 TLPALRR11 pKa = 11.84 TLRR14 pKa = 11.84 AQTPLTTLNRR24 pKa = 11.84 PFSSLLTSRR33 pKa = 11.84 PSLPTTTITLTSTLSPLLSPLTSTLQSPIQSQLQSRR69 pKa = 11.84 QFSASASLAGKK80 pKa = 10.15 RR81 pKa = 11.84 ITFNPSRR88 pKa = 11.84 RR89 pKa = 11.84 VQKK92 pKa = 10.2 RR93 pKa = 11.84 RR94 pKa = 11.84 HH95 pKa = 5.33 GFLARR100 pKa = 11.84 LRR102 pKa = 11.84 TRR104 pKa = 11.84 GGRR107 pKa = 11.84 KK108 pKa = 8.11 ILMRR112 pKa = 11.84 RR113 pKa = 11.84 RR114 pKa = 11.84 QRR116 pKa = 11.84 GKK118 pKa = 10.57 KK119 pKa = 9.12 ALSWW123 pKa = 3.66
Molecular weight: 13.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.491
IPC2_protein 11.52
IPC_protein 13.115
Toseland 13.276
ProMoST 13.773
Dawson 13.276
Bjellqvist 13.276
Wikipedia 13.759
Rodwell 12.881
Grimsley 13.32
Solomon 13.773
Lehninger 13.685
Nozaki 13.276
DTASelect 13.276
Thurlkill 13.276
EMBOSS 13.788
Sillero 13.276
Patrickios 12.603
IPC_peptide 13.788
IPC2_peptide 12.778
IPC2.peptide.svr19 9.39
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11244
1
11245
4932616
49
6421
438.6
48.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.093 ± 0.018
1.278 ± 0.011
5.718 ± 0.017
6.312 ± 0.025
3.791 ± 0.012
6.786 ± 0.021
2.452 ± 0.01
4.925 ± 0.015
4.728 ± 0.019
8.918 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.207 ± 0.008
3.844 ± 0.012
6.094 ± 0.028
4.171 ± 0.016
6.091 ± 0.019
8.287 ± 0.027
5.882 ± 0.015
6.126 ± 0.016
1.447 ± 0.008
2.85 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here