Mycobacterium phage IPhane7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mclasvirinae; Bongovirus; unclassified Bongovirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385D3X7|A0A385D3X7_9CAUD Uncharacterized protein OS=Mycobacterium phage IPhane7 OX=2301552 GN=73 PE=4 SV=1
MM1 pKa = 7.82SDD3 pKa = 3.48TPPAPVSASGRR14 pKa = 11.84WVKK17 pKa = 10.47IGSASVDD24 pKa = 3.35SGTLAVIDD32 pKa = 3.83PCYY35 pKa = 10.8VIGKK39 pKa = 9.75GEE41 pKa = 4.54EE42 pKa = 3.77IYY44 pKa = 10.85GWNDD48 pKa = 2.95YY49 pKa = 11.42DD50 pKa = 4.5SLHH53 pKa = 6.91EE54 pKa = 4.53PYY56 pKa = 10.27CDD58 pKa = 3.3NLGVQFSSGFGDD70 pKa = 3.76GAYY73 pKa = 9.56EE74 pKa = 3.81VWAWIVDD81 pKa = 3.63MGDD84 pKa = 2.82GFMGGEE90 pKa = 3.66RR91 pKa = 11.84VAEE94 pKa = 3.96VRR96 pKa = 11.84VCLISACEE104 pKa = 4.54DD105 pKa = 3.51GDD107 pKa = 5.2DD108 pKa = 5.46DD109 pKa = 5.72GDD111 pKa = 3.93HH112 pKa = 6.79

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385D2Z5|A0A385D2Z5_9CAUD Terminase large subunit OS=Mycobacterium phage IPhane7 OX=2301552 GN=14 PE=4 SV=1
MM1 pKa = 7.69ALALLKK7 pKa = 10.68APNVPMPAHH16 pKa = 7.1VYY18 pKa = 8.88RR19 pKa = 11.84VHH21 pKa = 7.46RR22 pKa = 11.84RR23 pKa = 11.84ACPNPWKK30 pKa = 10.5DD31 pKa = 3.38RR32 pKa = 11.84YY33 pKa = 6.69TTAAEE38 pKa = 3.79AHH40 pKa = 6.03RR41 pKa = 11.84VVTDD45 pKa = 3.3ILTRR49 pKa = 11.84NKK51 pKa = 8.66HH52 pKa = 4.44TKK54 pKa = 9.7KK55 pKa = 10.36NRR57 pKa = 11.84IPQGVYY63 pKa = 8.66EE64 pKa = 4.32CRR66 pKa = 11.84CGGFHH71 pKa = 7.37LSDD74 pKa = 3.29ARR76 pKa = 11.84RR77 pKa = 11.84VVALGGG83 pKa = 3.49

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

137

0

137

23057

29

2273

168.3

18.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.231 ± 0.472

0.88 ± 0.133

6.692 ± 0.183

6.428 ± 0.243

3.101 ± 0.144

8.345 ± 0.372

2.038 ± 0.168

4.858 ± 0.183

4.472 ± 0.215

8.067 ± 0.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.143

3.565 ± 0.165

4.936 ± 0.197

3.665 ± 0.205

6.367 ± 0.282

5.317 ± 0.182

6.111 ± 0.212

7.061 ± 0.252

2.32 ± 0.152

3.019 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski